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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,227 results for: sgRNA

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Showing: 8561 - 8580 of 9227 results

  7. B-Sniper SpCas9

    Plasmid
    #207361
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-Sniper SpCas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    F539S, M763I, K890N, amino acids 1005-1013 replac…
    Promoter
    CBh
    Available since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207361-plasmid-map-sequence-id-410129

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  11. htCRISPRi-Puro

    Pooled library
    #254479
    Purpose
    Genome-scale CRISPRi library for inhibiting transcription of expressed genes in human T cell with 2 sgRNAs/gene; expresses puromycin resistance gene.
    Depositor
    Alexander Marson
    Publication
    Zhu et al Cell. 2026 Aug 28:S0092-8674(26)00929-3.
    Expression
    Mammalian
    Species
    Homo sapiens
    Use
    CRISPR and Lentiviral
    Available since
    July 16, 2026
    Availability
    Academic Institutions and Nonprofits only

  12. htCRISRPi-Puro-GFP

    Pooled library
    #254480
    Purpose
    Genome-scale CRISPRi library for inhibiting transcription of expressed genes in human T cell with 2 sgRNAs/gene; expresses both puromycin resistance gene and GFP.
    Depositor
    Alexander Marson
    Publication
    Zhu et al Cell. 2026 Aug 28:S0092-8674(26)00929-3.
    Expression
    Mammalian
    Species
    Homo sapiens
    Use
    CRISPR and Lentiviral
    Available since
    July 16, 2026
    Availability
    Academic Institutions and Nonprofits only

  15. pODN-H2BC11-mR2

    Plasmid
    #183866
    Purpose
    Repair template for the C-terminal tagging of H2B histones with mRuby2 in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2BC11 homology arms with linker-mRuby2 (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mRuby2
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Available since
    July 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    183866-plasmid-map-sequence-id-368215

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  16. pODN-mR2-MYH10

    Plasmid
    #183869
    Purpose
    Repair template for the N-terminal tagging of myosin heavy chain 10 with mRuby2 in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MYH10 homology arms with mRuby2-linker (MYH10 Human)
    Use
    CRISPR; Donor template
    Tags
    mRuby2-linker
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183869-plasmid-map-sequence-id-362448

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Showing: 8561 - 8580 of 9227 results