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pEGFP-N1 Dynamin1 R290A D291A T292A

Plasmid

#112103

Purpose

Mammalian expression of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

R290A D291A T292A

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 T292A L293A P294A

Plasmid

#112104

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T292A L293A P294A

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 L330R Q334R L702R

Plasmid

#112105

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

L330R Q334R L702R

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 T78R L84R T92R V118R

Plasmid

#112106

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T78R L84R T92R V118R

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 Dynamin1 D406A M407A

Plasmid

#112107

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D406A M407A

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 Dynamin1 D406A M407A F409A

Plasmid

#112108

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D406A M407A F409A

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 Dynamin1 D406R M407R

Plasmid

#112109

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D406R M407R

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 Dynamin1 D406R M407R F409A

Plasmid

#112110

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D406R M407R F409A

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 Dynamin1 D406R M407R T488W

Plasmid

#112111

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D406R M407R T488W

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJPN0003

Plasmid

#112080

Purpose

GFP module NT2

Depositor

Christian Chevalier

Insert

GFP

Use

Tags

Expression

Plant

Mutation

BsaI and BpiI restriction sites have been remove…

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pORFE1001

Plasmid

#112079

Purpose

Overexpression of the AtCAS9 in plant under 2x35S promoter

Depositor

Christian Chevalier

Insert

AtCAS9

Use

CRISPR

Tags

Expression

Plant

Mutation

Plant codon optimized Cas9 S. pyogenes

Promoter

2x35S

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pORFE1052

Plasmid

#112078

Purpose

Overexpression of the AtCAS9 nickase in plant under 2x35S promoter

Depositor

Christian Chevalier

Insert

AtCAS9 D10A

Use

CRISPR

Tags

Expression

Plant

Mutation

Plant codon optimized nCas9 D10A S. pyogenes

Promoter

2x35S

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pJPN0018

Plasmid

#112083

Purpose

eCFP module CT

Depositor

Christian Chevalier

Insert

eCFP

Use

Tags

Expression

Plant

Mutation

BsaI and BpiI restriction sites have been remove…

Promoter

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-3

Plasmid

#112843

Purpose

transient/stable overexpression of the C-terminal domain of Catulin.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Catulin-alpha-1 (Ctnnal1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

C2 domain of Catulin

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-1

Plasmid

#112841

Purpose

transient/stable overexpression of the N-terminal domain of Catulin.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Catulin-alpha-1 (Ctnnal1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

N domain of Catulin

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(WT)

Plasmid

#112720

Purpose

Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(WT)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(P46A)

Plasmid

#112721

Purpose

This mutation (P46A) marks the first residue within the evolved region of U1A.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P46A)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

P46A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(R47A)

Plasmid

#112722

Purpose

Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47A)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

R47A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(R47K)

Plasmid

#112723

Purpose

Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47K)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

R47K

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(Q48A)

Plasmid

#112724

Purpose

Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48A)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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