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Plasmid#24259DepositorInsertBmf (S87D) (BMF Human)
UseTagsFlagExpressionMammalianMutationchanged Serine 74 to Aspartic AcidPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag BimL (T56D)
Plasmid#24237DepositorInsertBimL (T56D) (BCL2L11 Human)
UseTagsFlagExpressionMammalianMutationChanged threonine 56 to aspartic acidPromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag BimL (S58D)
Plasmid#24239DepositorInsertBimL (S58D) (BCL2L11 Human)
UseTagsFlagExpressionMammalianMutationChanged serine 58 to aspartic acidPromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag BimL (S44D)
Plasmid#24235DepositorInsertBimL (S44D) (BCL2L11 Human)
UseTagsFlagExpressionMammalianMutationChanged serine 44 to aspartic acidPromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 BimL (T56D)
Plasmid#24532DepositorInsertBim L(T56D) (BCL2L11 Human)
UseTagsT7ExpressionMammalianMutationChanged threonine 56 to aspartic acidPromoterAvailable sinceAug. 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-STAG2-D299A
Plasmid#31975DepositorInserthuman STAG2 cDNA (STAG2 Human)
UseTagsEGFP and FLAGExpressionMammalianMutationchanged Aspartic acid 299 to AlaninePromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLANT-2b
Plasmid#231487PurposeExpresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.DepositorInsertsUseTagsHis6 tag and His6-SUMO^NITExpressionBacterialMutationDeleted amino acids 1-6 (MEPEES). and M83L, E84Q,…PromoterT7 promoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
Human DsRed-Monomer CASP9 C287A Mutant
Plasmid#198469PurposeExpresses Human DsRed-Monomer CASP9 C287A Mutant Fusion Protein in Mammalian CellsDepositorInsertHuman Caspase-9 C287A Mutant (CASP9 Human)
UseTagsDsRed-monomerExpressionMammalianMutationChanged Cysteine 287 to Alanine to eliminate prot…PromoterCMVAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET29a(+)::D1141A
Plasmid#192963PurposeTest the activity of the putative aspartyl protease motif DPXGL-(18)-DPXGLDepositorInsertTse5_D1141A
UseTags9xhis + TEV tagExpressionBacterialMutationD1141APromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB205
Plasmid#169116PurposeTESEC expression vector for Asd from M. tuberculosisDepositorInsertasd (asd Mycobacterium tuberculosis H37Rv)
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E. coliPromoterpBADAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
AlkB-D135G
Plasmid#160434PurposeA plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to GlyDepositorInsertE. coli AlkB
UseTagsHis-tagExpressionBacterialMutationChanged Aspartic acid 135 to Glycine; deleted the…PromoterT7Available sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
FoxFTVC+bpFOG_MEK-S216D, S220E
Plasmid#107522PurposeConstitutive activation of FGF/MAPK pathway by MEK-S216D, S220E mutantDepositorInsertMEK
UseTagsExpressionMutationchanged Serine 216 to Aspartic Acid and Serine 22…PromoterTVC-specific FoxF enhancer (FoxFTVC+bpFOG)Available sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Thy1.1-muSOX D219A
Plasmid#131704Purposeencodes Thy1.1 followed by a P2A site and muSOX D219A catalytic mutant for enriching pure populations of transfected cellsDepositorInsertThy1.1-muSOX
UseTagsExpressionMammalianMutationAspartic acid 219 mutated to an alaninePromoterCMVAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
ORF18(G145A_N146A)-3xFLAG pCDNA4
Plasmid#129812PurposeExpresses KSHV ORF18 (G145A_N146A) with Cterm 3xFLAG tag in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseTags3xFLAGExpressionMammalianMutationchanged Glycine 145 to Alanine and Asparagine 146…PromoterCMVAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
ORF18(N63A)-3xFLAG pCDNA4
Plasmid#129780PurposeExpresses KSHV ORF18 (N63A) with Cterm 3xFLAG tag in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseTags3xFLAGExpressionMammalianMutationchanged Asparagine 63 to AlaninePromoterCMVAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
ORF18(N146A)-3xFLAG pCDNA4
Plasmid#129798PurposeExpresses KSHV ORF18 (N146A) with Cterm 3xFLAG tag in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseTags3xFLAGExpressionMammalianMutationchanged Asparagine 146 to AlaninePromoterCMVAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK513
Plasmid#121470PurposeCEN LEU2 CNB1mutEF1DepositorInsertCNB1mutEF1 (CNB1 Budding Yeast)
UseYeast cen plasmidTagsExpressionMutationChanged aspartic acid 34 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK511
Plasmid#121468Purpose2µm HIS3 CNB1mutEF4DepositorInsertCNB1mutEF4 (CNB1 Budding Yeast)
UseYeast high copy 2 micron vectorTagsExpressionMutationChanged aspartic acid 144 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK510
Plasmid#121465Purpose2µm HIS3 CNB1mutEF3DepositorInsertCNB1mutEF3 (CNB1 Budding Yeast)
UseYeast high copy 2 micron vectorTagsExpressionMutationChanged aspartic acid 103 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK509
Plasmid#121464Purpose2µm HIS3 CNB1mutEF2DepositorInsertCNB1mutEF2 (CNB1 Budding Yeast)
UseYeast high copy 2 micron vectorTagsExpressionMutationChanged aspartic acid 66 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only