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Plasmid#179558Purposeencodes S. pyogenes dCas9 with n-terminal fusion of human CBP core (aa 1084-1701) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoterDepositorInsertUseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only
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pAAV-EF1a-iCreV (AAV1)
Viral Prep#140135-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-EF1a-iCreV (#140135). In addition to the viral particles, you will also receive purified pAAV-EF1a-iCreV plasmid DNA. EF1a-driven, light-inducible iCreV recombinase for spatiotemporally precise optogenomic modifications. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-RUNX2(WT)-IDR-mCherry
Plasmid#145286PurposeBacterial Expression of 6xHis RUNX2(WT) IDR mCherry Fusion ProteinDepositorInsertRUNX2(WT)-IDR (RUNX2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFUSE-DEST_R1R4
Plasmid#97186PurposeFor fusion gene cloning. Recombine Gene A + Gene B into this Gateway Destination VectorDepositorTypeEmpty backboneUseLentiviral; GatewayTagsV5ExpressionMammalianMutationPromoterEF1 alphaAvailable sinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-RUNX2(+10A)-IDR-mCherry
Plasmid#145287PurposeBacterial Expression of 6xHis RUNX2(+10A) IDR mCherry Fusion ProteinDepositorInsertRUNX2(+10A)-IDR (RUNX2 Human)
UseTagsExpressionBacterialMutationA-repeat expandedPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
GoldyTALEN-ppp1cab TAL1
Plasmid#41835DepositorInsertprotein phosphatase 1, catalytic subunit, alpha isoform b (ppp1cab Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
GoldyTALEN-ppp1cab TAL2
Plasmid#41836DepositorInsertprotein phosphatase 1, catalytic subunit, alpha isoform b (ppp1cab Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pRGD-HmR
Plasmid#74107PurposePlasmid containing hygromycin B resistance gene cassette flanked by multiple restriction sitesDepositorInserthph5
UseBacterial vector based on pmb1 repliconTagsExpressionMutationPromoterAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-MPP8-delta55-118-10xHis
Plasmid#194180PurposeExpresses MPP8-delta55-118_10xHisDepositorInsertMPHOSPH8 (MPHOSPH8 Human)
UseTags10xHisExpressionMammalianMutationMPP8-delta55-118 (chromo domain deletion)PromoterCMVAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag-Par6 delta 250-295 Flag tagged
Plasmid#24646DepositorInsertPar6c (Pard6a Mouse)
UseTagsFLAGExpressionMammalianMutationDeletion of the PDZ domain of Par6c (aa 250-295)PromoterAvailable sinceJune 2, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENTR1A_mmFbxw7(res) (closed)
Plasmid#160105PurposeExpresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNADepositorInsertFbxw7alpha (Fbxw7 Mouse)
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX304_zeo_mmFbxw7(res)
Plasmid#160106PurposeExpresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNADepositorInsertFbxw7alpha (Fbxw7 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTag4C_Nrg1-GV-2HA
Plasmid#227104PurposeNrg1 protein for luciferase and barcode assaysDepositorInsertNrg1-GV (Nrg1 Mouse)
UseTags2xHAExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-CD8a(7A)
Plasmid#162498PurposeExpresses GFP tagged CD8a mutant in mammalian cells. The seven O-glycosylation sites in its stem region are mutated.DepositorInsertCD8a with a mutated stem region (CD8A Human)
UseTagsGFPExpressionMammalianMutationThe five threonine and two serine residues are mu…PromoterAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-TRIM24-C840W
Plasmid#28136DepositorInsertTRIM24 (TRIM24 Human)
UseTagsFLAGX2ExpressionMammalianMutationA489V and C840WPromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-iCreV (AAV PHP.eB)
Viral Prep#140135-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-EF1a-iCreV (#140135). In addition to the viral particles, you will also receive purified pAAV-EF1a-iCreV plasmid DNA. EF1a-driven, light-inducible iCreV recombinase for spatiotemporally precise optogenomic modifications. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk2a2(apf)
Plasmid#13761DepositorInsertJnk2a2(apf) (MAPK9 Human)
UseTagsFlagExpressionMammalianMutationreplaced dual phosporylation motif THr(183)-Pro-T…PromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1(+)-RIα-EGFP
Plasmid#181840PurposeGFP-tagged PKA RIα subunit for mammalian expression.DepositorInsertRIα-EGFP (PRKAR1A Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only