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Plasmid#59967PurposeAn AAV packaging vector that expresses hPREP under control of the EF1a promoter.DepositorInsertProlyl oligopeptidase (PREP Human)
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
E. coli BL21 (DE3) ΔEntD
Bacterial Strain#192874PurposeDeletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.DepositorBacterial ResistanceNoneSpeciesEscherichia coliAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pJExpress414_GmPOPB
Plasmid#92234PurposeExpress wild type prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationnonePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-S577A
Plasmid#92235PurposeExpress S577A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-S577A mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationSerine 577 to AlaninePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-D661A
Plasmid#92236PurposeExpress D661A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-D661A mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationAspartate 661 to AlaninePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-H698N
Plasmid#92242PurposeExpress H698N mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-H698N mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationHistidine 698 to AsparaginePromoterT7 promoterAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-R663K
Plasmid#92239PurposeExpress R663K mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-R663K mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationArginine 663 to LysinePromoterT7 promoterAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-W695del
Plasmid#92241PurposeExpress W695-deletion mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-W695deletion mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationTryptophan 695 deletedPromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-R663Q
Plasmid#92240PurposeExpress R663Q mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-R663Q mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationArginine 663 to GlutaminePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-H698A
Plasmid#92237PurposeExpress H698A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-H698A mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationHistidine 698 to AlaninePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-R663A
Plasmid#92238PurposeExpress R663A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-R663A mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationArginine 663 to AlaninePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AviTag-SnoopLigase
Plasmid#105626PurposePlasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylationDepositorInsertAviTag-SnoopLigase
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HaloTag7-SnoopLigase
Plasmid#105627PurposePlasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7DepositorInsertHaloTag7-SnoopLigase
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDF-Leup
Plasmid#213154PurposeExpresses the leupeptin protease inhibitor pathway for bolstered production of heterologously expressed proteins.DepositorInsertLeupeptin Protease Inhibitor Pathway
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pUC57-BBclone1
Plasmid#137069PurposeE. coli plasmid that contains the expression optimized genes for BB0405, BB0406, P66, BBA57, HtrA, RevA and BB0238DepositorInsertsUseTagsExpressionBacterialMutationwild-type, full-length (signal-peptide-containing…PromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHLSec2-irisin-his
Plasmid#122729PurposeExpresses in mammalian cells (HEK293):glycosylated and secreted irisin-hisDepositorInsertirisin (ectodomain of FNDC5) (Fndc5 Human, Mouse, Bovine, Rat)
UseTagshis and signal sequence for secretionExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only