Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 1,801 results for: nif

Clear keywords
Showing: 841 - 860 of 1801 results

  2. pOpen-HSlig3

    Plasmid
    #165574
    Purpose
    Repairs single strand breaks in DNA efficiently. Unable to perform either blunt-end joining or AMP- dependent relaxation of supercoiled DNA (Elder, R.H. et al . , Bur. J. Biochem.. 203:53-58 (1992))
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    DNA Ligase III
    Use
    Synthetic Biology
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165574-plasmid-map-sequence-id-340976

    Enlarge

  4. L0I101- GG4-pGATA23-Bxb1-tUBQ10-GG6

    Plasmid
    #219712
    Purpose
    Level 0 pGATA23-Bxb1-tUBQ10 construct for assembly of two integrase constructs, GG4 and GG6 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG4-pGATA23-Bxb1-tUBQ10-GG6
    Use
    Synthetic Biology
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219712-plasmid-map-sequence-id-435863

    Enlarge

  5. L1T15 - T2PsB (mtagBFP2, mCherry, NeonGreen)

    Plasmid
    #219689
    Purpose
    Level 1 preswitched History-dependent target without promoter, switch by Bxb1, output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2PsB (mtagBFP2, mCherry, NeonGreen)
    Expression
    Plant
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219689-plasmid-map-sequence-id-435724

    Enlarge

  6. L0I90 - GG0-pSPCH-PhiC31-tUBQ1-GG4

    Plasmid
    #219709
    Purpose
    Level 0 pSPCH-PhiC31-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pSPCH:PhiC31:tUBQ1-GG4
    Use
    Synthetic Biology
    Available Since
    June 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    219709-plasmid-map-sequence-id-435862

    Enlarge

  7. L0I84 - GG0-pAHP6-GG1

    Plasmid
    #219706
    Purpose
    Level 0 pAHP6 promoter, GG0 and GG1 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pAHP6-GG1
    Use
    Synthetic Biology
    Mutation
    WT promoter with added golden gate linkers
    Available Since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219706-plasmid-map-sequence-id-435736

    Enlarge

  8. L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)

    Plasmid
    #219687
    Purpose
    Level 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
    Expression
    Plant
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219687-plasmid-map-sequence-id-435722

    Enlarge

  9. L1T9 - T2PB/p35S (mTagBFP2, mCherry, NeonGreen)

    Plasmid
    #219686
    Purpose
    Level 1 History-dependent target, switch PhiC31 then Bxb1, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2PB/p35S (mTagBFP2, mCherry, NeonGreen)
    Expression
    Plant
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219686-plasmid-map-sequence-id-436048

    Enlarge

  10. pOpen-Exonuclease III E. coli

    Plasmid
    #165571
    Purpose
    Double-stranded DNA specific exonuclease. Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases. Initiates at nicked sites in double-stranded DNA.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Exonuclease III E. coli
    Use
    Synthetic Biology
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165571-plasmid-map-sequence-id-340929

    Enlarge

  11. L1PB1 - pSPCH--PhiC31-tUBQ1 - pMUTE--Bxb1-tUBQ10

    Plasmid
    #219699
    Purpose
    Level 1 PhiC31 and Bxb1 integrase construct for tracking of stomata development: pSPCH promoter for PhiC31 and pMUTE promoter for Bxb1, no tag
    Depositor
    Jennifer Nemhauser
    Insert
    pSPCH::PhiC31:tUBQ1 - pMUTE::Bxb1:tUBQ10
    Expression
    Plant
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219699-plasmid-map-sequence-id-435730

    Enlarge

  12. L1T11 - T2BP/p35S (BFP, mCherry, NeonGreen)

    Plasmid
    #219688
    Purpose
    Level 1 History-dependent target, switch Bxb1 then PhiC31, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2BP/p35S (BFP, mCherry, NeonGreen)
    Expression
    Plant
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219688-plasmid-map-sequence-id-435723

    Enlarge

  13. L0I97 - GG0-pAHP6-PhiC31-NLS-tUBQ1-GG4

    Plasmid
    #219711
    Purpose
    Level 0 pAHP6-PhiC31-NLS-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pAHP6:PhiC31-NLS:tUBQ1-GG4
    Use
    Synthetic Biology
    Available Since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219711-plasmid-map-sequence-id-435738

    Enlarge

  14. pOpen-ECOligA

    Plasmid
    #165564
    Purpose
    Connects preferentially cohesive double-stranded DNA ends, active on blunt end DNA in the presence of Ficoll or polyethylene glycol. Requires Mg2+ and NAD+. Ligation when blunt end or RNA/ DNA ligation needs to be avoided.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    E. coli DNA Ligase
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165564-plasmid-map-sequence-id-340043

    Enlarge

  15. L1T18 - T2PsB/p35S preswitched (mTagBFP2, mCherry, NeonGreen)

    Plasmid
    #219690
    Purpose
    Level 1 preswitched History-dependent target, switch with Bxb1, p35S promoter, and output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2PsB/p35S (mTagBFP2, mCherry, NeonGreen)
    Expression
    Plant
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219690-plasmid-map-sequence-id-435857

    Enlarge

  17. TGN38 EGFP

    Plasmid
    #128148
    Purpose
    Expression of TGN38 amino acids 304-357
    Depositor
    Jennifer Lippincott-Schwartz
    Insert
    TGN38 (Tgoln2 Rat)
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Contains amino acids 304-357
    Available Since
    Nov. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    128148-plasmid-map-sequence-id-251067

    Enlarge

  18. pOpen-GBDpol (exo)

    Plasmid
    #165572
    Purpose
    Robust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEB
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Pyrococcus Sp. Heat-Stable (exo-) DNA Polymerase
    Use
    Synthetic Biology
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165572-plasmid-map-sequence-id-340932

    Enlarge

  19. pOpen-T4 g32

    Plasmid
    #165560
    Purpose
    A single-stranded DNA binding protein required for bacteriophage T4 replication and repair; Used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structure, to increase yield and processivity of reverse transcriptase during RT-PCR, and to increase yield and specificity of PCR products from soil samples.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    T4 gene 32
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165560-plasmid-map-sequence-id-339953

    Enlarge

  20. pOpen-BovDNTT

    Plasmid
    #165580
    Purpose
    Adds dT at the end of PCR blunted DNA fragments/vectors that can then be used in TOPO cloning; Addition of homopolymer tails to the 3' ends of DNA; Labeling the 3' ends of DNA with modified nucleotides (e.g., ddNTP, DIG-dUTP); TUNEL assay (in situ localization of apoptosis); TdT dependent PCR
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Bovine DNTT
    Use
    Synthetic Biology
    Available Since
    July 28, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165580-plasmid-map-sequence-id-338558

    Enlarge

Showing: 841 - 860 of 1801 results