We narrowed to 6,443 results for: plasmid dna

1. pcDNA3.1-N-Myc_535-888 USP7

   Plasmid

   #131248

   Purpose

   Mammalian expression of the USP7 UBL1-3 domains with an N-terminal Myc tag.

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-N-Myc_535-775 USP7

   Plasmid

   #131247

   Purpose

   Mammalian expression of the USP7 UBL1-2 domains with an N-terminal Myc tag.

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-sACE2v2.4(732)-IgG1

   Plasmid

   #154106

   Purpose

   Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (IgG1-Fc fusion)

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Fc region of human IgG1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease and neck domains …

   Promoter

   CMV

   Available since

   June 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5-FRT-TO-Nprot-HF_GC3opt

   Plasmid

   #157732

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nprot-HF_GC3opt (N SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pcDNA3-sACE2v2(732)-IgG1

   Plasmid

   #154105

   Purpose

   Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (IgG1-Fc fusion)

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Fc region of human IgG1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease and neck domains …

   Promoter

   CMV

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-sACE2-T92Q-Fc(IgG1)

   Plasmid

   #145170

   Purpose

   Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) glycosylation mutant T92Q

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Fc region of human IgG1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA-FRT-TO-FH-Nsp8-Nsp7_GC3opt

   Plasmid

   #157701

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pcDNA3-sACE2-T92Q-sfGFP

   Plasmid

   #145178

   Purpose

   Mammalian expression plasmid for sACE2-sfGFP glycosylation mutant T92Q

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Linker GSGGSGSGG and superfolder GFP

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-N-Myc_DW164AA 1-205 USP7

   Plasmid

   #131254

   Purpose

   Mammalian expression of a mutant USP7 TRAF-like domain (DW164AA), with an N-terminal Myc tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Expresses a mutant form of our pcDNA3.1-N-Myc_1-2…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-sACE2-T92Q-Fc(IgA1)

    Plasmid

    #145162

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) glycosylation mutant T92Q

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Fc region of human IgA1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pESRRG.1.0-gDNA

    Plasmid

    #132473

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ESRRG (ESRRG Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDNA3.1_3XFlag

    Plasmid

    #182494

    Purpose

    Empty plasmid containing Flag tag for C-terminal tagging

    Depositor

    Paolo Comoglio

    Article

    Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

    Insert

    3xFlag with 5 Glycolic spacer
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNA3.0_mitoSNAP

    Plasmid

    #136623

    Purpose

    Plasmid encoding outer mitochondrial localization SNAP-Tag

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    NTOM20-SNAP-Tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.3_SARS2_omicron BA.2

    Plasmid

    #183700

    Purpose

    expressing SARS-CoV-2 BA.2 spike protein for pseudovirus production

    Depositor

    David Nemazee

    Article

    Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

    Insert

    Spike (BA.2) (S SARS-CoV-2)
    

    Use

    Lentiviral and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    c-terminal 18 aa deletion

    Promoter

    CMV

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.3_SARS2_Mu

    Plasmid

    #177578

    Purpose

    expressing SARS-CoV-2 B.1.621 (Mu) spike protein for pseudovirus production

    Depositor

    David Nemazee

    Article

    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

    Insert

    Spike (Mu variant) (S SARS-CoV-2)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    T95I, Y144T, Y145S, R346K, E484K, N501Y, D614G, …

    Promoter

    CMV

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.3_SARS2_Lambda

    Plasmid

    #177579

    Purpose

    expressing SARS-CoV-2 C.37 (lambda) spike protein for pseudovirus production

    Depositor

    David Nemazee

    Article

    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

    Insert

    Spike (Lambda variant) (S SARS-CoV-2)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    75-76VI, 246-253N, L452Q, F490S, D614G, T859N, la…

    Promoter

    CMV

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA4TO-3XFLAG Halo R99A nsp1

    Plasmid

    #175422

    Purpose

    Expresses R99A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 R99A nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTFAM.1.0-gDNA

    Plasmid

    #132443

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    TFAM (TFAM Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pARID4B.1.0-gDNA

    Plasmid

    #132436

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ARID4B (ARID4B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGTF2I.1.0-gDNA

    Plasmid

    #132452

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    GTF2I (GTF2I Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge