We narrowed to 1,853 results for: rigi

1. pSLICER

   Plasmid

   #197288

   Purpose

   Conjugative plasmid that facilitates seamless deletions in D. radiodurans following insertion of a seamless deletion cassette. Contains an origin of transfer

   Depositor

   Bogumil Karas

   Article

   Brumwell et al Biodes Res. 2023 Mar 15;5:0009. doi: 10.

   Insert

   I-SceI Endonuclease
   

   Use

   Synthetic Biology; Bacterial and yeast cloning

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJUMP27-1A(sfGFP)

   Plasmid

   #126974

   Purpose

   Level 1 vector. sfGFP reporter. Origin pSC101 (low copy number)

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJUMP46-2A(sfGFP)

   Plasmid

   #126990

   Purpose

   Level 2 vector. sfGFP reporter. Origin p15A (medium copy number)

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available Since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBBR6k-GFPuv

   Plasmid

   #106384

   Purpose

   IPTG-inducible LlacO-1 promoter expressing gfpuv on BBR1 origin

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   LlacO-1

   Available Since

   March 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCJH019_SC101_pLAC_Cas12c(4)_Kan

   Plasmid

   #183071

   Purpose

   Wild-type Cas12c_4 in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   Cas12c_4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Wild-type

   Promoter

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJUMP28-1A(sfGFP)

   Plasmid

   #126976

   Purpose

   Level 1 vector. sfGFP reporter. Origin pUC (high copy number)

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pL2-G(P)10G

   Plasmid

   #137105

   Purpose

   iFLinkC Linker cloning

   Depositor

   Viktor Stein

   Article

   Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

   Insert

   G(P)10G
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pL2-G(TP)4TG

   Plasmid

   #137101

   Purpose

   iFLinkC Linker cloning

   Depositor

   Viktor Stein

   Article

   Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

   Insert

   G(TP)4TG
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pL2-GPPPG

   Plasmid

   #137094

   Purpose

   iFLinkC Linker cloning

   Depositor

   Viktor Stein

   Article

   Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

   Insert

   GPPPG
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJUMP26-1A(sfGFP)

    Plasmid

    #126973

    Purpose

    Level 1 vector. sfGFP reporter. Origin p15A (medium copy number)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pES133

    Plasmid

    #127552

    Purpose

    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13

    Depositor

    Emmanuele Severi, Gavin Thomas

    Insert

    F5-flanked tetracycline resistance cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Multiple SNPs in tetA (originally from pBR322) to…

    Promoter

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCJH021_SC101_pLAC_Cas12c2_Kan

    Plasmid

    #183072

    Purpose

    Wild-type Cas12c2 (Yan et al., 2019) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRO16Sh2

    Plasmid

    #180604

    Purpose

    Expression of ShCAST under control of Plac with pRO1600 origin.

    Depositor

    Christopher Reisch

    Article

    Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

    Insert

    ShTnsB, ShTnsC, ShTniQ, ShCas12k
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLac

    Available Since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pILxR5

    Plasmid

    #149497

    Purpose

    Broad-Host range expression vector, pUC origin, Tn7 recognition sites, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    LuxR-mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLuxB

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJUMP48-2A(sfGFP)

    Plasmid

    #126993

    Purpose

    Level 2 vector. sfGFP reporter. Origin pUC (high copy number)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mV-VfAU1-LOV_226

    Plasmid

    #58686

    Purpose

    Expresses mVenus-fusion protein of VfAU1-LOV domain (Vaucheria frigida Aureochrome 1)

    Depositor

    Harald Janovjak

    Article

    Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

    Insert

    mV-VfAU1-LOV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBBR6k-con-GFPuv

    Plasmid

    #106387

    Purpose

    Constitutive LlacO-1 promoter expressing gfpuv on BBR1 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LlacO-1

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNCS-Flag

    Plasmid

    #17364

    Depositor

    Stephen Goff

    Article

    Yueh et al J Virol. 2003 Feb . 77(3):1820-9.

    Insert

    MMLV proviral DNA
    

    Use

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available Since

    March 1, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pKCyGe2

    Plasmid

    #167515

    Purpose

    Broad-Host range expression vector, RK2 and pUC origin, CymRAM repressor, PCymRC promoter controlling GmR, Kanamycin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    CymRAM-GmR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PCymRC

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXLK_aFb

    Plasmid

    #114230

    Purpose

    POC12368, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type a-b

    Depositor

    Christopher A. O'Callaghan

    Article

    Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

    Insert

    LacZalpha
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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