We narrowed to 32,761 results for: eng

1. pGT-Ah7

   Plasmid

   #122574

   Purpose

   MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   catP/sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGT-Ah10

   Plasmid

   #122577

   Purpose

   MAGIC donor plasmid (Himar transposon with tetQ/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   tetQ/sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGT-Ah6

   Plasmid

   #122573

   Purpose

   MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   catP/sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPR-Cas9-ScaligD

   Plasmid

   #125688

   Purpose

   Gene knockout/in for actinomycetes

   Depositor

   Tilmann Weber

   Article

   Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

   Insert

   streptomyces codon optimized spCas9, sgRNA casette, and ScaligD
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   ermE*/tipA

   Available Since

   Nov. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. gag-TadCBEd

   Plasmid

   #225144

   Purpose

   Packaging plasmid for TadCBEd in v3 PE-eVLP architecture

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   MMLVgag-TadCBEd
   

   Expression

   Mammalian

   Available Since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. hPGK-rtTA3

   Plasmid

   #195883

   Purpose

   Lentiviral construct of hPGK driven rtTA3

   Depositor

   Elaine Fuchs

   Article

   Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

   Insert

   rtTA3
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBsaBM3

   Plasmid

   #85102

   Purpose

   P450 BM3 variant (A74G/C773S/C810S)

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   P450 BM3
   

   Use

   Cloning vector

   Mutation

   A74G/C773S/C810S

   Available Since

   Dec. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFH3.29_Cre

   Plasmid

   #205560

   Purpose

   Express Cre (without export tag)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   Cre
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6_N7-sgRNA2

   Plasmid

   #190664

   Purpose

   Expresses U6-driven N7 sgRNA2 (poly-T mutated out), BsmbI dropout cassette for cloning

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   N7 sgRNA2
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3xFLAG-NanoLuc

    Plasmid

    #189975

    Purpose

    Part 3-4-5 plasmid to introduce 3xFLAG-NanoLuc into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    NanoLuc
    

    Use

    Cloning

    Tags

    3xFLAG

    Promoter

    N/A

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT2/shp53/GFP4

    Plasmid

    #20208

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    shp53/GFP4
    

    Use

    Transposon

    Available Since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLH-sgRNA1-2XMS2

    Plasmid

    #75389

    Purpose

    sgRNA1-2XMS2

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XMS2
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRE-mCherry-p2A-Chrimson-TS

    Plasmid

    #158702

    Purpose

    Reporter construct

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    TRE-mcherry-p2A-Chrimson-TS
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNX32-Dest

    Plasmid

    #105083

    Purpose

    Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

    Depositor

    Christopher Grefen

    Article

    Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

    Type

    Empty backbone

    Tags

    NubG

    Expression

    Yeast

    Available Since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRE-mCherry-p2A-bReaches-TS

    Plasmid

    #158701

    Purpose

    Reporter construct

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    mCherry-p2A-bReaches-TS
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEC2483 (pSpy1C)

    Plasmid

    #220248

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. K-SPOTIT1.0

    Plasmid

    #171215

    Purpose

    Expresses a kappa-opioid receptor-based circular permuted green fluorescent protein sensor

    Depositor

    Wenjing Wang

    Article

    Kroning et al Angew Chem Int Ed Engl. 2021 Mar 4. doi:

    Insert

    K-SPOTIT1.0
    

    Use

    Lentiviral

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRSET-mSA-EGFP

    Plasmid

    #39862

    Depositor

    Sheldon Park

    Article

    Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

    Insert

    monomeric streptavidin fused to EGFP
    

    Tags

    6xHis, EGFP, and FLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKM491-Flag-His

    Plasmid

    #109282

    Purpose

    ORBIT integrating plasmid for C-terminal tagging with FLAG-His tag

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    attB-Flag-His
    

    Use

    Orbit integrating plasmid

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHen2 Avi-tag-linker-p3

    Plasmid

    #119819

    Purpose

    For cloning and subsequent expression of peptide fragments fused with Avi-tag (for biotinylation) and with pIII protein of M13K07 bacteriophage

    Depositor

    Alexander Gabibov

    Article

    Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

    Insert

    linker with Avi-tag and p3
    

    Tags

    Avi-tag

    Expression

    Bacterial

    Available Since

    May 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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