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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 23,030 results for: his

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Showing: 8601 - 8620 of 23030 results

  1. MALT1 (3BFO)

    Plasmid
    #25216
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    malt1.226.314 (MALT1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acid residues 226-314 of Isoform 2
    Available Since
    Aug. 12, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. pET28(a)-hFX(I154F)-HA

    Plasmid
    #14992
    Depositor
    Michael Ristow
    Insert
    human frataxin cDNA (FXN Human)
    Tags
    AU1, HA, and HIS
    Expression
    Bacterial
    Mutation
    contains cDNA coding sequence for amino acids 88-…
    Available Since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  3. pET22b-DS55

    Plasmid
    #187175
    Purpose
    This modified pET22b-DS55 vector contains Sal I and Hind III sites in reverse orientations to directly clone scFv phagemid from HuScL-4 library for bacterial expression systems.
    Depositor
    Dinakar Salunke
    Article
    Jaiswal et al bioRxiv 2025.07.15.663531
    Type
    Empty backbone
    Tags
    His tag
    Expression
    Bacterial
    Promoter
    AmpR, lac1
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    187175-plasmid-map-sequence-id-371430

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  9. pTE5415

    Plasmid
    #229030
    Purpose
    Expression vector for 10xHis-phi29 DNA polymerase (wild-type): PT7-lacO-g10leader-p2 wt-T7 terminator, CamR
    Depositor
    Tobias Erb
    Article
    Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/
    Insert
    PT7-lacO-g10lead-10xHisTag-phi29 DNAP_wt
    Tags
    10x His-tag + Factor Xa recognition site
    Expression
    Bacterial
    Promoter
    PT7-lacO and T7 promoter
    Available Since
    July 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    229030-plasmid-map-sequence-id-466058

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  10. pTE5444

    Plasmid
    #231064
    Purpose
    Expression vector for 10x-phi29 DNA polymerase (PovSak): PT7-lacO-g10leader-10xHis-p2 povsak-T7 terminator, CamR
    Depositor
    Tobias Erb
    Article
    Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/
    Insert
    PT7-lacO-g10lead-10xHisTag-phi29 DNAP_PovSak
    Tags
    10x His-tag + Factor Xa recognition site
    Expression
    Bacterial
    Promoter
    PT7-lacO and T7 promoter
    Available Since
    July 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    231064-plasmid-map-sequence-id-466059

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  11. pTE5448

    Plasmid
    #231065
    Purpose
    Expression vector for 10x-phi29 DNA polymerase (PovSakOk): PT7-lacO-g10leader-10xHis-p2 povsakok-T7 terminator, CamR
    Depositor
    Tobias Erb
    Article
    Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/
    Insert
    PT7-lacO-g10lead-10xHisTag-phi29 DNAP_PovSakOk
    Tags
    10x His-tag + Factor Xa recognition site
    Expression
    Bacterial
    Promoter
    PT7-lacO and T7 promoter
    Available Since
    July 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    231065-plasmid-map-sequence-id-466057

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  13. pET28a-RANGAP1-CTD

    Plasmid
    #228947
    Purpose
    express the C-terminal domain of the human RANGAP1 protein (residues 418-587 of UniProt P46060 with the N-terminal thrombin-cleavable MGSSHHHHHHSSGLVPR|GSHM- tag). Codon-optimized
    Depositor
    Marcin Suskiewicz
    Insert
    RANGAP1-CTD (RANGAP1 Human)
    Tags
    His tag and thrombine cleavage site : MGSSHHHHHHS…
    Expression
    Bacterial
    Mutation
    contains aa 418-587 only
    Promoter
    T7-lac
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    228947-plasmid-map-sequence-id-472386

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  15. pCCG_nit9fl_Gephyrin_linker_reverse

    Plasmid
    #228417
    Purpose
    Expression of NIT-9 domains in reverse orientation with Gephyrin linkage region in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9 domains reversed (GPHN Human)
    Use
    Protein expression in n. crassa
    Mutation
    removal of the linkage region and insertion of th…
    Available Since
    April 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228417-plasmid-map-sequence-id-460294

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  16. EV-A71 isolate 4643-TW98 2A precursor protease with C110A and four aa at N-term to create a VP1-2A junction in active site

    Plasmid
    #228633
    Purpose
    Bacterial expression of the A71 2A protease with ITTL at the N-terminal and a C110A mutation used for crystallization in P 6 1 (PDB 9FGO)
    Depositor
    ASAP Discovery, Frank von Delft
    Insert
    Enterovirus Coxsackievirus A71 2A protease
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    C110A
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228633-plasmid-map-sequence-id-464103

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Showing: 8601 - 8620 of 23030 results