We narrowed to 23 results for: his
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Plasmid Kit#1000000223PurposeA suite of Saccharomyces cerevisiae strain construction vectors for perturbing gene expression at the transcriptional, post-transcriptional, and post-translational levels.DepositorAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only
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Yeast Secrete and Detect
Plasmid Kit#1000000166PurposeYeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.DepositorAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human Kinase Domain Constructs for Automated Bacterial Expression
Plasmid Kit#1000000094Purpose68 His-tagged human kinase catalytic domain constructs and the respective phosphatases that enhance their bacterial expression; optimized for automated bacterial expressionDepositorAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ubiquiton In Vitro Kit
Plasmid Kit#1000000242PurposeThe Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.DepositorAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
4G-Cloning Plasmid Kit
Plasmid Kit#1000000252PurposeThe 4G (Golden-Gate-guided Gibson) cloning kit provides elements for rapid one-day assembly of multi-gene expression vectors in bacterial and eukaryotic expression hosts.DepositorAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ubiquiton Yeast Kit
Plasmid Kit#1000000243PurposeThe Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.DepositorAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ubiquiton Human Kit
Plasmid Kit#1000000244PurposeThe Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.DepositorAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
FNL Combinatorial Cloning Platform
Plasmid Kit#1000000211PurposeA collection of Multisite Gateway Entry clone plasmids which can be used in a combinatorial fashion to generate large numbers of tagged expression clones for protein expression in a variety of systemsDepositorAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Target Accelerator Pan-Cancer Mutant Collection
Plasmid Kit#1000000103PurposePanel of cancer alleles that have undergone basic, functional screening; full-length, human clones (454 mutant and 177 corresponding wild-type) in the Gateway cloning systemDepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
MultiSite Gateway plasmid toolKit
Plasmid Kit#1000000050PurposeCloning kit for constructing fully-customizable three-part fusion proteins, based on MultiSite Gateway from Life Technologies. 12 promoter-less Gateway entry clones, N- and C-term flanking modulesDepositorAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
EcoFlex MoClo Kit
Plasmid Kit#1000000080PurposeGolden Gate kit for E. coli synthetic biology; consists of a modular library that uses MoClo assembly standard for a variety of uses, e.g. recombinant protein purification, cell-free circuit designDepositorAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMVP and pMAGIC Cloning Systems
Plasmid Kit#1000000155PurposepMVP and pMAGIC are cloning systems for adenoviral, lentiviral, expression, PiggyBac transposon, and Sleeping Beauty transposon vectors for transgene or RNAi delivery and dCas9-based engineering.DepositorAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
The Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit
Plasmid Kit#1000000210PurposeToolkit contains Cas9-gRNA vectors, integration plasmids, and organellar localization markers as a set of plasmids for Cas9-mediated integration of tagged genes and markers into the yeast genome.DepositorAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Yeast Prototrophy Kit
Plasmid Kit#1000000085PurposePlasmids derived from the pHLUM mini-chromosome, designed to restore prototrophy (eg HIS3, LEU2, URA3, MET17 or LYS2 genes) in common yeast (S. cerevisiae) laboratory strains.DepositorAvailable sinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
The Expanded CRISPEY Kit
Plasmid Kit#1000000257PurposeUse the Expanded CRISPEY Kit for CRISPR-based gene editing of S. cerevisiae.DepositorAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
AID* Kit
Plasmid Kit#1000000120PurposeExpanded tool kit for the auxin-inducible degron (AID) system in yeast ( S. cerevisiae). Includes tags for easy detection by antibodies/microscopy and a set of selection markers.DepositorAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCut plasmid toolkit
Plasmid Kit#1000000118PurposeCas9-based kit in S. cerevisiae for optimizing heterologous gene expression. Cas9-sgRNA (pCut) plasmids for integrating into 23 loci, across all 16 yeast chromosomes.DepositorAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
Yeast GoldenBraid Cloning System and Toolkit
Plasmid Kit#1000000138PurposeModular cloning system for generation of high expression transcriptional units; Integrates in yeast (S. cerevisiae) genome at two different loci supporting high and stable transgene expressionDepositorAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
CRISPaint Gene Tagging Kit
Plasmid Kit#1000000086PurposeModular system for integrating large DNA fragments into defined genomic locations. Canonical NHEJ mechanism. For tagging of endogenous proteins with luciferase, fluorescent proteins, epitope tagsDepositorAvailable sinceSept. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
Diatom uLoop Assembly Kit
Plasmid Kit#1000000238PurposeThe collection contains a selection of diatom promoters and terminators from the organism Phaeodactylum tricornutum on vectors compatible with the uLoop cloning system.DepositorAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only