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We narrowed to 222 results for: his

Showing: 1 - 20 of 222 results
  1. To Each HIS Own

    Type
    Blog Post
    ...interfere with protein function.  Additional Benefits of HIS tags In addition to providing a way to purify a protein...fused to polyhistidine, then they can use an anti-HIS antibody, like this one developed by Dr. James Trimmer...
  2. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...current mechanistic understanding of GFP’s function. His lab described the protein’s structure and also discovered...local pet shop aquariums in Moscow where he lived! His hunch paid off, and he was able to report new fluorophores... Katushka/mKate from Entacmaea quadricolor. With his discoveries, another part of the fluorescent protein...
  3. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...was also a trained biologist. This is apparent in his work The Island of Dr. Moreau (1896) where he explores...attributed to SciFi writer Jack Williamson who used it in his 1951 novel Dragon’s Island. In 1976, the world’s ...wrong. The plot of the movie involves The Rock and his super giant albino gorilla fighting off a monstrous...
  4. Video Library

    Type
    Protocol
    ...discuss the various positions he has held in his career, his favorite company tradition, and Addgene's role...Development Manager, discusses his transition from the Scientist Team to Business Team, his hobbies, and advice ...
  5. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Trono 14666 pQE9-His-p97(wt) VCP His T5 ALS Graham Warren 14667 pQE9-His-p97(QQ) VCP His T5 ALS Graham Warren...17229 pQE9-His-p97deltaD2 VCP His T5 ALS Hemmo Meyer 17230 pQE9-His-p97deltaD2(K251A) VCP His T5 ALS Hemmo...0005 VCP His T7 ALS Axel Brunger 12339 pBD-0071 VCP His T7 ALS Axel Brunger 12344 pBD-0010 VCP His T7 ALS...0016 VCP His T7 ALS Axel Brunger 12346 pBD-0013 VCP His T7 ALS Axel Brunger 12347 pBD-0006 VCP His T7 ALS...0009 VCP His T7 ALS Axel Brunger 12349 pBD-0070 VCP His T7 ALS Axel Brunger 12350 pBD-0069 VCP His T7 ALS...0008 VCP His T7 ALS Axel Brunger 12352 pBD-0007 VCP His T7 ALS Axel Brunger 12353 pBD-0018 VCP His T7 ALS...0020 VCP His T7 ALS Axel Brunger 12355 pBD-0022 VCP His T7 ALS Axel Brunger 12356 pBD-0024 VCP His T7 ALS...
  6. AAV Production in HEK293 Cells

    Type
    Protocol
    ... 2, Corning 3269, 1272 cm 2 Heat-inactivated FBS (HI-FBS) Pro-Tip Different brands and lots of FBS can... sorbitol (optional) : DMEM low glucose + 1% v/v HI-FBS + 0.1 M sorbitol In our hands, we have found ... M sorbitol, add 57 mL of 1.46 M sorbitol, 7.5 mL HI-FBS, and 7.5 mL 100X glutaGRO, 7.5 mL of 7.5% Sodium...sterile 250 mL bottle. Aliquot 774 mL of DMEM + 2% HI-FBS into a sterile 1 L bottle. Add each plasmid DNA...solution to the bottle containing 774 mL of DMEM + 2% HI-FBS. Mix well. Take the CS5 out of the incubator...
  7. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Drosophila transgenesis His Epitope tag pEZYmyc-His - C-terminal Myc-His tag for mammalian expression...Gateway destination vector for bacterial expression; His,GST tag pE2c - Modified pENTR vector for C-terminal...the extracellular surface of S. cerevisiae cells His-tagged versions of pFastBac LIC vectors and pFastBac...pNH-TrxT - LIC cloning in bacteria with N-terminal His-TrxA-TEV tag NusA Protein purification pNIC-HIS6-...of protein in E. coli as TEV cleavable N-terminal His and NusA fusions Return to top Function Besides functions...
  8. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian Expression...dimerization pCEP4YPet-MAMM - Mammalian Expression pYPet-His - Bacterial Expression YPet-N1 - Mammalian Expression..._miRFP670 - Mammalian Lentiviral Expression pBAD/His-miRFP670 - Bacterial Expression iRFP670 643 670 13...pAAV-hSYN-miRFP670nano3-WPRE-hGHpA - Mammalian AAV Expression pBAD/His-miRFP670nano3 - Bacterial Expression miRFP680 661...Monomer pmiRFP703-N1 - Mammalian Expression pBAD/His-miRFP703 - Bacterial Expression iRFP702 673 702 7.4...Monomer pmiRFP709-N1 - Mammalian Expression pBAD/His-miRFP709 - Bacterial Expression mIFP 683 704 7 3.5....5 2.8 h Dimer piRFP - Mammalian Expression pBAD/His-B-iRFP - Bacterial Expression miRFP713 690 713 7 ...
  9. Biosensor AAV Preps

    Type
    Collection
    ...pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Syn CaMPARI2 his Constitutive 1 Eric Schreiter 101061 pAAV_hsyn_NES-his-CaMPARI2...F391W-WPRE-SV40 Syn CaMPARI2-F391W his Constitutive 1 Eric Schreiter 101064 pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40...-SV40 Syn CaMPARI2-L398T his Constitutive 1 Eric Schreiter Calcium Sensor: jRGECO1 100852 pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40...
  10. Institute for Protein Innovation

    Type
    Collection
    ...and those targeting V5, Protein C, DYKDDDDK, Rho, His, Biotin, EE, GCN4 and Strep. Please visit our Tags...
  11. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Biosensor Eric Schreiter AV-1-PV4704 101064-AAV1 pAAV_hsyn_NES-his-CaMPARI2... Schreiter AV-1-PV4705 101061-AAV1 pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40 Biosensor Eric Schreiter...
  12. Bacterial Expression Systems

    Type
    Collection
    ...Fluorescent Protein Application PI 14030 14031 pCyPet-His pYPet-His CyPet (donor) YPet (acceptor) FRET Patrick Daugherty...
  13. TALEN Guide

    Type
    Collection
    ...21822235 . See the Q&A with Dr. Adam Bogdanove and his thoughts on the TALe technology here ....
  14. Molecular Biology Reference

    Type
    Guide
    ..., GAG Glycine Gly G GGU, GGC, GGA, GGG Histidine His H CAU, CAC Isoleucine Ile I AUU, AUC, AUA Leucine...Tag Amino Acid Sequence FLAG DYKDDDDK HA YPYDVPDYA His HHHHHH Myc EQKLISEEDL V5 GKPIPNPLLGLDST Xpress DLDDDDK...
Showing: 1 - 20 of 222 results