...discuss the various positions he has held in his career, his favorite company tradition, and Addgene's role...Development Manager, discusses his transition from the Scientist Team to Business Team, his hobbies, and advice ...

... 2, Corning 3269, 1272 cm 2 Heat-inactivated FBS (HI-FBS) Pro-Tip Different brands and lots of FBS can... sorbitol (optional) : DMEM low glucose + 1% v/v HI-FBS + 0.1 M sorbitol In our hands, we have found ... M sorbitol, add 57 mL of 1.46 M sorbitol, 7.5 mL HI-FBS, and 7.5 mL 100X glutaGRO, 7.5 mL of 7.5% Sodium...sterile 250 mL bottle. Aliquot 774 mL of DMEM + 2% HI-FBS into a sterile 1 L bottle. Add each plasmid DNA...solution to the bottle containing 774 mL of DMEM + 2% HI-FBS. Mix well. Take the CS5 out of the incubator...

...Trono 14666 pQE9-His-p97(wt) VCP His T5 ALS Graham Warren 14667 pQE9-His-p97(QQ) VCP His T5 ALS Graham Warren...17229 pQE9-His-p97deltaD2 VCP His T5 ALS Hemmo Meyer 17230 pQE9-His-p97deltaD2(K251A) VCP His T5 ALS Hemmo...51641 ADAM10-bio-His ADAM10 His, CD4 CMV Alzheimer's Gavin Wright 51643 APP-bio-His APP His, CD4 CMV Alzheimer's...0005 VCP His T7 ALS Axel Brunger 12339 pBD-0071 VCP His T7 ALS Axel Brunger 12344 pBD-0010 VCP His T7 ALS...0016 VCP His T7 ALS Axel Brunger 12346 pBD-0013 VCP His T7 ALS Axel Brunger 12347 pBD-0006 VCP His T7 ALS...0009 VCP His T7 ALS Axel Brunger 12349 pBD-0070 VCP His T7 ALS Axel Brunger 12350 pBD-0069 VCP His T7 ALS...0008 VCP His T7 ALS Axel Brunger 12352 pBD-0007 VCP His T7 ALS Axel Brunger 12353 pBD-0018 VCP His T7 ALS...

...required. pET-HIS-Sangamo 40786 TALEN Gang Bao Gateway cloning and expression of an N-terminally His-tagged ...pET-Sangamo-His 40787 TALEN Gang Bao Gateway cloning and expression of a C-terminally His-tagged TALEN...fluorescent protein variant (YPet) found in pYPet-His . pYPet-His 14031 FRET Patrick Daugherty FRET: Vector for... and his3. In bacterial strain USO hisB- pyrF- rpoZ- , this promotes growth on plates lacking His and ...Tag(s) ID Purpose pDEST-HisMBP 11085 Tac (lactose/IPTG inducible) MBP, 6x His David Waugh Empty lactose...N-terminal 6x His tag and an MBP tag. Compatible with Gateway cloning. pDEST-periHisMBP 11086 Tac (lactose... for super resolution imaging, epitope tags like His for easy western blotting or staining, and RNA transcript...

...Prey Cd4d3+4-COMP-b-lac His-tag Cd4d3+4-6his Bio-linker-his Cd4d3-bio-linker-6His* FLAG prey CD200-Cd4d3...COMP-blac-3xFLAG-6his Cd4d3+4-pentamer BasiginCd4d3+4-COMP-his-stop Type II expression CD69-His-bio Multi TM... TM expression CD36-bio-His Plasmid collections exist for: Basic AVEXIS : A list of all you need to get...Martin S, Söllner C, Charoensawan V, Adryan B, Thisse B, Thisse C, Teichmann S and Wright GJ Molecular & cellular...

... optimized CyPet pCyPet-His Cyan Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian...mammalian optimized YPet pYPet-His Yellow Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian...Cyan Bacterial Expresses Aquamarine with N-terminal His tag pAquaN1 Cyan Mammalian Expresses mammalian optimized...

... CRISPR CRISPR History CRISPR History and Development for Genome Engineering ...

...mammalian expression 6xHis Epitope Tag pEZYmyc-His - C-terminal Myc-His tag for mammalian ... His tagged bacterial expression vectors pET His6 TEV - N-terminal His6-TEV tag ...Cloning (LIC) Bacteria Lac, T7, araBAD p15TV-L - His-tagged bacterial expression vector... series - Expression with Strep, His or optimized YFP tags for purification...tag for bacterial expression pET His6 - C-terminal His6 tag for bacterial expression pDest... mammalian expression pET His6 GST TEV - N-terminal His6-GST-TEV for bacterial ...expression pNIC-CTHF - C-terminal TEV-His6-Flag for bacterial expression pET Flag TEV - N-terminal...

...and cloned into an IPTG-inducible His-tag expression vector. All His-tags are cloned at the C-terminus...inexpensive to assay, and easy to purify using histidine-tagged constructs and routine protein and molecular...map, a TEV cleavage site is present between the 6xHis-tag and the coding region of MDH. For detailed clone...

...pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Syn CaMPARI2 his Constitutive 1 Schreiter 101061 pAAV_hsyn_NES-his-CaMPARI2...F391W-WPRE-SV40 Syn CaMPARI2-F391W his Constitutive 1 Schreiter 101064 pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40...L398T-WPRE-SV40 Syn CaMPARI2-L398T his Constitutive 1 Schreiter Calcium Sensor: jRGECO1 100852 pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40...-ChrimsonR-ST Syn jGCaMP8s none Cre-dependent 9 Histed 176749 AAV-Syn-H2B-jGCaMP8s-WPRE Syn jGCaMP8s none...

...Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian Expression...dimerization pCEP4YPet-MAMM - Mammalian Expression pYPet-His - Bacterial Expression YPet-N1 - Mammalian Expression...Monomer pmiRFP670-N1 - Mammalian Expression pBAD/His-miRFP670 - Bacterial Expression iRFP670 643 670 13...Monomer pmiRFP703-N1 - Mammalian Expression pBAD/His-miRFP703 - Bacterial Expression iRFP702 673 702 8...Monomer pmiRFP709-N1 - Mammalian Expression pBAD/His-miRFP709 - Bacterial Expression mIFP 683 704 7 3.5...4.5 2.8 hr Dimer piRFP - Mammalian Expression pBAD/His-B-iRFP - Bacterial Expression iSplit 690 713 5 Dimer...tdTomato - Mammalian Expression pFA6a-link-tdTomato-SpHis5 - Yeast Expression tdTomato-N1 - Mammalian Expression...

...Benefits of HIS tags In addition to providing a way to purify a protein of interest, polyhisitidine tags can...protein of interest is fused to polyhistidine, then they can use an anti-HIS antibody, like this one developed...purification based on the polyhistidine tag. What is the polyhistidine tag? The polyhistidine tag is a string of....      Fig 2:  A, The polyhistidine tag is a string of histidine amino acids on the N- or C- terminus...residues Deciding how many histidine residues to use is a bit easier. The more histidine residues that are present... low level, try a longer histidine tag to increase capture. Longer histidine tags are also recommended... of 6 or more histidine residues expressed in frame on the N- or C-terminus of a recombinant protein of...

...antibodies targeting V5, Protein C, DYKDDDDK, Rho, His, Biotin, EE, GCN4 and Strep. Please visit our Tags...

... lead his team as a scientists and a friend. George Church runs his lab fairly similarly to his advisor...the majority of his time in the lab.  As time went on and he was spending more time in his office, he gradually... always “off flying his plane to exotic places while occasionally emailing us his worldly advice”, the...package that included a postdoc and a PhD student in his first year. “It was very important to me to be with...adviser did? Tom Ellis merges the influence from his two PhD advisers who were “chalk-and-cheese”. While...

...contributed much of his knowledge to the CRISPR resources at Addgene. His background in history and specifically...spent most of his research career working on miRNAs and RNAi. He first heard about RNAi at his MIT interview... to the interview here! Doench majored in history during his undergraduate work at Hamilton College. When...that that’s the thing for you.” After recognizing his love for science and later realizing that he wanted...After a few rotations, Doench decided to complete his PhD work in Sharp’s lab.  Doench says, “what I loved...process. This early research is still relevant to his work at the Broad Institute today, where he helps... questions. Starting at the Broad Institute For his post-doc, Doench worked on using RNAi screens to ...

...be found on his website http://www.microworldsphotography.com/. Another artist who spent his life blurring...2008. His work exposes and explores the hidden structures within flowers with the use of x-rays. His haunting... non-academic people.” Daniel went on to discuss his work: “Macro photography has been my passion for ...Richards, a teacher of dental radiography, had focused his x-ray machine not on teeth, but on blossoms, thus...unique samples, such as those collected by Darwin on his voyage. Previous destructive techniques were incompatible...

... life. His lab has long hypothesized that life started with RNA and a simple cell membrane. His recent...depositor) Craig Mello. Taking his groundbreaking work in RNAi one step further, his lab is now studying how ...Tet-on system, his lab has created a tetracycline cas9 inducible knock-out system. His lab is also exploring...presented by Todd Lowe from UCSC. Dr. Lowe talked about his work on the 500+ human tRNA genes and, until now,...role in prokaryotic immunity to viral invasion.  His lab is studying P. furiosus, a hyperthermophile that...resistant bacteria. See the picture to the left for his list of the bacterial species that “ESKAPE” antibiotic... structural studies on the ribosome performed in his lab. RNA is a structural and functional component...

...he hopes others will find his tools useful, including his zinc sensor and his genetically encoded magnesium...on the move, such as T lymphocytes. Griesbeck and his colleagues began by isolating a calcium-binding protein...Ratiometric imaging for beginners Griesbeck says his calcium indicators can be used in mouse, Drosophila...Griesbeck also points out that the approach he and his colleagues took to optimize sensitivities can now...other published FRET-based sensors. For example, his team is now applying it to improve upon an indicator...than calcium, requiring greater sensitivity. He and his colleagues came up with a solution: they devised ...

...covered! His His tags, often called polyhistidine tags, typically include six consecutive His residues...more). Histidine bonds with immobilized metal ions (Nickel, Cobalt, Copper, etc.), allowing His fusion...step metal affinity chromatography. Purification of His fusion proteins from E. coli is generally more straightforward...an antibody for that!   Fig. 1 - Diagram of His-tagged protein purification work flow by nickel column...Protein tags Antibodies 101: Epitope tags To Each HIS Own ...due to the presence of more naturally occurring histidine-rich proteins in mammals. Mammalian purification...

...same kinds of opportunities for his students. But he also found that his teaching responsibilities left...limited time for one-on-one mentoring. His solution? Bringing his research into the classroom. I had the...to hear about how he developed his program. Initially, Provost made his labs completely open-ended, allowing...San Diego), decided to take a different approach. His own most impactful learning experiences happened ... opportunity to talk with Provost recently about his development of what is now commonly termed a Course-based...encouraging them to engage in the problem-solving process. His previous experiences as a jazz musician, serviceman...serviceman in the U.S. Army, and hockey coach all shaped his teaching style, with the former offering excellent...