We narrowed to 582 results for: his

...discuss the various positions he has held in his career, his favorite company tradition, and Addgene's role...Development Manager, discusses his transition from the Scientist Team to Business Team, his hobbies, and advice ...

... 2, Corning 3269, 1272 cm 2 Heat-inactivated FBS (HI-FBS) Pro-Tip Different brands and lots of FBS can... sorbitol (optional) : DMEM low glucose + 1% v/v HI-FBS + 0.1 M sorbitol In our hands, we have found ... M sorbitol, add 57 mL of 1.46 M sorbitol, 7.5 mL HI-FBS, and 7.5 mL 100X glutaGRO, 7.5 mL of 7.5% Sodium...sterile 250 mL bottle. Aliquot 774 mL of DMEM + 2% HI-FBS into a sterile 1 L bottle. Add each plasmid DNA...solution to the bottle containing 774 mL of DMEM + 2% HI-FBS. Mix well. Take the CS5 out of the incubator...

...Trono 14666 pQE9-His-p97(wt) VCP His T5 ALS Graham Warren 14667 pQE9-His-p97(QQ) VCP His T5 ALS Graham Warren...17229 pQE9-His-p97deltaD2 VCP His T5 ALS Hemmo Meyer 17230 pQE9-His-p97deltaD2(K251A) VCP His T5 ALS Hemmo...0005 VCP His T7 ALS Axel Brunger 12339 pBD-0071 VCP His T7 ALS Axel Brunger 12344 pBD-0010 VCP His T7 ALS...0016 VCP His T7 ALS Axel Brunger 12346 pBD-0013 VCP His T7 ALS Axel Brunger 12347 pBD-0006 VCP His T7 ALS...0009 VCP His T7 ALS Axel Brunger 12349 pBD-0070 VCP His T7 ALS Axel Brunger 12350 pBD-0069 VCP His T7 ALS...0008 VCP His T7 ALS Axel Brunger 12352 pBD-0007 VCP His T7 ALS Axel Brunger 12353 pBD-0018 VCP His T7 ALS...0020 VCP His T7 ALS Axel Brunger 12355 pBD-0022 VCP His T7 ALS Axel Brunger 12356 pBD-0024 VCP His T7 ALS...

... CRISPR CRISPR History CRISPR History and Development for Genome Engineering ...

...Drosophila transgenesis His Epitope tag pEZYmyc-His - C-terminal Myc-His tag for mammalian expression...bacteria with N-terminal His-TrxA-TEV tag NusA Protein purification pNIC-HIS6-NUSA-TEV-GG - Cloning of...Gateway destination vector for bacterial expression; His,GST tag pE2c - Modified pENTR vector for C-terminal...the extracellular surface of S. cerevisiae cells His-tagged versions of pFastBac LIC vectors and pFastBac.... coli as TEV cleavable N-terminal His and NusA fusions Return to top Function Besides functions such ... with cap-dependent 3Myc-EYFP-HA-His6 and IRES-dependent ECFP-HA-His6 translation for mammalian expression... mammalian expression pET His6 GST TEV - N-terminal His6-GST-TEV for bacterial ...

...Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian Expression...dimerization pCEP4YPet-MAMM - Mammalian Expression pYPet-His - Bacterial Expression YPet-N1 - Mammalian Expression..._miRFP670 - Mammalian Lentiviral Expression pBAD/His-miRFP670 - Bacterial Expression iRFP670 643 670 13...pAAV-hSYN-miRFP670nano3-WPRE-hGHpA - Mammalian AAV Expression pBAD/His-miRFP670nano3 - Bacterial Expression miRFP680 661...Monomer pmiRFP703-N1 - Mammalian Expression pBAD/His-miRFP703 - Bacterial Expression iRFP702 673 702 7.4...Monomer pmiRFP709-N1 - Mammalian Expression pBAD/His-miRFP709 - Bacterial Expression mIFP 683 704 7 3.5....5 2.8 h Dimer piRFP - Mammalian Expression pBAD/His-B-iRFP - Bacterial Expression miRFP713 690 713 7 ...

...and cloned into an IPTG-inducible His-tag expression vector. All His-tags are cloned at the C-terminus...inexpensive to assay, and easy to purify using histidine-tagged constructs and routine protein and molecular...map, a TEV cleavage site is present between the 6xHis-tag and the coding region of MDH. For detailed clone...

...pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Syn CaMPARI2 his Constitutive 1 Eric Schreiter 101061 pAAV_hsyn_NES-his-CaMPARI2...F391W-WPRE-SV40 Syn CaMPARI2-F391W his Constitutive 1 Eric Schreiter 101064 pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40...-SV40 Syn CaMPARI2-L398T his Constitutive 1 Eric Schreiter Calcium Sensor: jRGECO1 100852 pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40...pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST Syn jGCaMP8s none Cre-dependent 9 Mark Histed 176749 AAV-Syn-H2B-jGCaMP8s-WPRE Syn jGCaMP8s none...

...Benefits of HIS tags In addition to providing a way to purify a protein of interest, polyhisitidine tags can...protein of interest is fused to polyhistidine, then they can use an anti-HIS antibody, like this one developed...purification based on the polyhistidine tag. What is the polyhistidine tag? The polyhistidine tag is a string of....      Fig 2:  A, The polyhistidine tag is a string of histidine amino acids on the N- or C- terminus...residues Deciding how many histidine residues to use is a bit easier. The more histidine residues that are present... low level, try a longer histidine tag to increase capture. Longer histidine tags are also recommended... of 6 or more histidine residues expressed in frame on the N- or C-terminus of a recombinant protein of...

...covered! His His tags, often called polyhistidine tags, typically include six consecutive His residues...more). Histidine bonds with immobilized metal ions (Nickel, Cobalt, Copper, etc.), allowing His fusion...step metal affinity chromatography. Purification of His fusion proteins from E. coli is generally more straightforward...an antibody for that!   Fig. 1 - Diagram of His-tagged protein purification work flow by nickel column...Protein tags Antibodies 101: Epitope tags To Each HIS Own ...due to the presence of more naturally occurring histidine-rich proteins in mammals. Mammalian purification...

...same kinds of opportunities for his students. But he also found that his teaching responsibilities left...limited time for one-on-one mentoring. His solution? Bringing his research into the classroom. I had the...to hear about how he developed his program. Initially, Provost made his labs completely open-ended, allowing...San Diego), decided to take a different approach. His own most impactful learning experiences happened ... opportunity to talk with Provost recently about his development of what is now commonly termed a Course-based...encouraging them to engage in the problem-solving process. His previous experiences as a jazz musician, serviceman...serviceman in the U.S. Army, and hockey coach all shaped his teaching style, with the former offering excellent...

...was also a trained biologist. This is apparent in his work The Island of Dr. Moreau (1896) where he explores...attributed to SciFi writer Jack Williamson who used it in his 1951 novel Dragon’s Island. In 1976, the world’s ...wrong. The plot of the movie involves The Rock and his super giant albino gorilla fighting off a monstrous...more blog posts about CRISPR Learn more about the history of CRISPR Read about other genome editing methods...

...Rett, who tried to raise awareness and published his observations in a German medical journal. Decades...to Rett Syndrome, this blog post describes the history of Rett Syndrome research, the role of the MECP2...

...Benefits of HIS tags In addition to providing a way to purify a protein of interest, polyhisitidine tags can...protein of interest is fused to polyhistidine, then they can use an anti-HIS antibody, like this one developed...purification based on the polyhistidine tag. What is the polyhistidine tag? The polyhistidine tag is a string of....      Fig 2:  A, The polyhistidine tag is a string of histidine amino acids on the N- or C- terminus...residues Deciding how many histidine residues to use is a bit easier. The more histidine residues that are present... low level, try a longer histidine tag to increase capture. Longer histidine tags are also recommended... of 6 or more histidine residues expressed in frame on the N- or C-terminus of a recombinant protein of...

...  Erik Snapp just released version 3.4 of his eBook, How to Get a Faculty Position. This edition has ...Snapp, who is always looking for ways to improve his eBook, adds: "If you have any questions, comments...email me at [email protected]." You can find his original blog post below! --Ed.  Eight years ago...formula to guarantee a reader the faculty job of his/her dreams. There are too many moving parts in the...luck with your search!   Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...Research Campus of the Howard Hughes Medical Institute. His interests include the quality control of secretory...

...antibodies targeting V5, Protein C, DYKDDDDK, Rho, His, Biotin, EE, GCN4 and Strep. Please visit our Tags...high-confidence targeting of proteins that have historically been difficult to identify and interrogate with...

... Embracing Serendipity Nicolas Mathis, who shared information on his open software that predicts efficiencies.... Illuminating Choices Paul Heisig, who shared his flow cytometry expertise in three posts. An Introduction...

... lead his team as a scientists and a friend. George Church runs his lab fairly similarly to his advisor...the majority of his time in the lab.  As time went on and he was spending more time in his office, he gradually... always “off flying his plane to exotic places while occasionally emailing us his worldly advice”, the...package that included a postdoc and a PhD student in his first year. “It was very important to me to be with...adviser did? Tom Ellis merges the influence from his two PhD advisers who were “chalk-and-cheese”. While...

...about his own biases--many of which will be shared by fellow scientists--but that doesn’t make his introduction...case studies are “old,” they lead Zimmer to one of his most critical observations: “Today the most successful...if Zimmer was able to rewrite the last chapter of his book today--the day you are reading this review--...

...contributed much of his knowledge to the CRISPR resources at Addgene. His background in history and specifically...spent most of his research career working on miRNAs and RNAi. He first heard about RNAi at his MIT interview... to the interview here! Doench majored in history during his undergraduate work at Hamilton College. When...that that’s the thing for you.” After recognizing his love for science and later realizing that he wanted...After a few rotations, Doench decided to complete his PhD work in Sharp’s lab.  Doench says, “what I loved...process. This early research is still relevant to his work at the Broad Institute today, where he helps... questions. Starting at the Broad Institute For his post-doc, Doench worked on using RNAi screens to ...