...Benefits of HIS tags In addition to providing a way to purify a protein of interest, polyhisitidine tags can...protein of interest is fused to polyhistidine, then they can use an anti-HIS antibody, like this one developed...purification based on the polyhistidine tag. What is the polyhistidine tag? The polyhistidine tag is a string of....      Fig 2:  A, The polyhistidine tag is a string of histidine amino acids on the N- or C- terminus...residues Deciding how many histidine residues to use is a bit easier. The more histidine residues that are present... low level, try a longer histidine tag to increase capture. Longer histidine tags are also recommended... of 6 or more histidine residues expressed in frame on the N- or C-terminus of a recombinant protein of...

... lead his team as a scientists and a friend. George Church runs his lab fairly similarly to his advisor...the majority of his time in the lab.  As time went on and he was spending more time in his office, he gradually... always “off flying his plane to exotic places while occasionally emailing us his worldly advice”, the...package that included a postdoc and a PhD student in his first year. “It was very important to me to be with...adviser did? Tom Ellis merges the influence from his two PhD advisers who were “chalk-and-cheese”. While...

...contributed much of his knowledge to the CRISPR resources at Addgene. His background in history and specifically...spent most of his research career working on miRNAs and RNAi. He first heard about RNAi at his MIT interview... to the interview here! Doench majored in history during his undergraduate work at Hamilton College. When...that that’s the thing for you.” After recognizing his love for science and later realizing that he wanted...After a few rotations, Doench decided to complete his PhD work in Sharp’s lab.  Doench says, “what I loved...process. This early research is still relevant to his work at the Broad Institute today, where he helps... questions. Starting at the Broad Institute For his post-doc, Doench worked on using RNAi screens to ...

...be found on his website http://www.microworldsphotography.com/. Another artist who spent his life blurring...2008. His work exposes and explores the hidden structures within flowers with the use of x-rays. His haunting... non-academic people.” Daniel went on to discuss his work: “Macro photography has been my passion for ...Richards, a teacher of dental radiography, had focused his x-ray machine not on teeth, but on blossoms, thus...unique samples, such as those collected by Darwin on his voyage. Previous destructive techniques were incompatible...

... life. His lab has long hypothesized that life started with RNA and a simple cell membrane. His recent...depositor) Craig Mello. Taking his groundbreaking work in RNAi one step further, his lab is now studying how ...Tet-on system, his lab has created a tetracycline cas9 inducible knock-out system. His lab is also exploring...presented by Todd Lowe from UCSC. Dr. Lowe talked about his work on the 500+ human tRNA genes and, until now,...role in prokaryotic immunity to viral invasion.  His lab is studying P. furiosus, a hyperthermophile that...resistant bacteria. See the picture to the left for his list of the bacterial species that “ESKAPE” antibiotic... structural studies on the ribosome performed in his lab. RNA is a structural and functional component...

...he hopes others will find his tools useful, including his zinc sensor and his genetically encoded magnesium...on the move, such as T lymphocytes. Griesbeck and his colleagues began by isolating a calcium-binding protein...Ratiometric imaging for beginners Griesbeck says his calcium indicators can be used in mouse, Drosophila...Griesbeck also points out that the approach he and his colleagues took to optimize sensitivities can now...other published FRET-based sensors. For example, his team is now applying it to improve upon an indicator...than calcium, requiring greater sensitivity. He and his colleagues came up with a solution: they devised ...

...covered! His His tags, often called polyhistidine tags, typically include six consecutive His residues...more). Histidine bonds with immobilized metal ions (Nickel, Cobalt, Copper, etc.), allowing His fusion...step metal affinity chromatography. Purification of His fusion proteins from E. coli is generally more straightforward...an antibody for that!   Fig. 1 - Diagram of His-tagged protein purification work flow by nickel column...Protein tags Antibodies 101: Epitope tags To Each HIS Own ...due to the presence of more naturally occurring histidine-rich proteins in mammals. Mammalian purification...

...same kinds of opportunities for his students. But he also found that his teaching responsibilities left...limited time for one-on-one mentoring. His solution? Bringing his research into the classroom. I had the...to hear about how he developed his program. Initially, Provost made his labs completely open-ended, allowing...San Diego), decided to take a different approach. His own most impactful learning experiences happened ... opportunity to talk with Provost recently about his development of what is now commonly termed a Course-based...encouraging them to engage in the problem-solving process. His previous experiences as a jazz musician, serviceman...serviceman in the U.S. Army, and hockey coach all shaped his teaching style, with the former offering excellent...

...youngster. His parents, Kristi and Rico, had to keep him from running too much as any jarring of his body could...further impairs the patient. When I first met AJ and his family, he did not seem much different than my second... able to run around and play like the other kids his age. A potential FOP cure But, there is HOPE. FOP...working at Sanofi Pharmaceuticals in Cambridge, MA. His work experience is in both vaccine subunit design... along with pre-clinical analytical development. His areas of expertise include early stage drug discovery...

...was also a trained biologist. This is apparent in his work The Island of Dr. Moreau (1896) where he explores...attributed to SciFi writer Jack Williamson who used it in his 1951 novel Dragon’s Island. In 1976, the world’s ...wrong. The plot of the movie involves The Rock and his super giant albino gorilla fighting off a monstrous...more blog posts about CRISPR Learn more about the history of CRISPR Read about other genome editing methods...

...up to be one of the best of the best students in his home country. He was selected as one of 600 scholars...of science in Horticulutre Biotechnology, he did his PhD in Plant Breeding, Genetics, and Biotechnology...Labs, for lab equipment that will help accelerate his research. Listen to learn more about Dr. Kwapata’...Kingdom's research 28:08-END : Kingdom's Thoughts on his time in Boston and Outro We'd like to thank Dr. ...

...about his clinical trial patients with advanced macular degeneration; Laurenz Studer discussed his work...Shinya Yamanaka celebrate the tenth anniversary of his landmark Cell paper showing that it was possible ...been looking forward to seeing how the sharing of his OSKM factors have affected the stem cell community...as "life changing". Dr. Yamanaka himself, during his keynote address, appeared almost incredulous that... citing the first clinical trial with iPSCs with his collaborator Takahashi in 2014 – just 8 years after...

...State University has been the go-to place. In 2012, his National High Magnetic Field Lab worked with an impressive... all those collaborations over the years, he and his colleagues built a Molecular Expressions collection...Davidson told the Tallahassee Democrat in a story about his life’s work and the stage IV lung cancer that has...has now prompted him to deposit the tools he and his team created with the research community at large,...to promote the great work of Michael Davidson and his colleagues." Acknowledgments Thank you to Michael...

...received his Bachelor of Science degree in Computer Engineering from Tufts University and his Master of...co-founder of Addgene and received his PhD in Computer Science from MIT. His research focused on computer systems...

...Rett, who tried to raise awareness and published his observations in a German medical journal. Decades...to Rett Syndrome, this blog post describes the history of Rett Syndrome research, the role of the MECP2...

...current mechanistic understanding of GFP’s function. His lab described the protein’s structure and also discovered...local pet shop aquariums in Moscow where he lived! His hunch paid off, and he was able to report new fluorophores... Katushka/mKate from Entacmaea quadricolor. With his discoveries, another part of the fluorescent protein...

...bacteriophages. He decided to quit his "safety net" at the VC firm and devoted 100% of his energy and effort into ...co-founder of a biotech startup, Mike explained, his career transitioned away from the bench and became...VP. Mike was fortunate enough to be able to spend his last 6 months in graduate school focusing on concept...self-sustainable. Within a year from this decision, Mike and his co-founders raised $6 million. The biggest challenge...money, the team grew, and Mike stepped down from his CEO position and became first COO, then moved on ...

...for any extrachromsomal hereditary determinant”. His proposal, however, was basically ignored. A separate...Learn more about the history of plasmids and cloning: CSHL Meeting: Plasmids: History & Biology The Joshua... history of plasmids...

...Packer - science writer  When Hans was finishing up his PhD in Molecular Neuroscience at the University of... like a lot of newly minted PhD’s: not sure what his next step should be. He debated taking a position... on the job. It keeps it interesting.” And while his official title may be science writer, writing is ...is just the one of the skills Hans uses for his job. “I am really more of a multimedia science communicator...started working on them shortly after joining IDT when his boss was looking for someone to take over hosting...