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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 4 results for: his

Showing: 1 - 4 of 4 results

  1. Video Library

    Type
    Protocol
    ...discuss the various positions he has held in his career, his favorite company tradition, and Addgene's role...Development Manager, discusses his transition from the Scientist Team to Business Team, his hobbies, and advice ...

  2. AAV Production in HEK293 Cells

    Type
    Protocol
    ... 2, Corning 3269, 1272 cm 2 Heat-inactivated FBS (HI-FBS) Pro-Tip Different brands and lots of FBS can... sorbitol (optional) : DMEM low glucose + 1% v/v HI-FBS + 0.1 M sorbitol In our hands, we have found ... M sorbitol, add 57 mL of 1.46 M sorbitol, 7.5 mL HI-FBS, and 7.5 mL 100X glutaGRO, 7.5 mL of 7.5% Sodium...sterile 250 mL bottle. Aliquot 774 mL of DMEM + 2% HI-FBS into a sterile 1 L bottle. Add each plasmid DNA...solution to the bottle containing 774 mL of DMEM + 2% HI-FBS. Mix well. Take the CS5 out of the incubator...

  3. Gibson Assembly Protocol

    Type
    Protocol
    ...TelesisBio. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO. (2009). Enzymatic assembly of DNA...Link opens in a new window) Gibson DG, Smith HO, Hutchison CA, Venter JC, Merryman C. (2010). Chemical synthesis...

  4. CRISPR Library Amplification

    Type
    Protocol
    ...Petri dish (VWR, 11019-552) 4 MaxiPreps (Qiagen HiSpeed Max, Catalog #12663) Tips (1000 µL, 200 µL, 10 ... Maxiprep. Purify plasmid DNA using the Qiagen HiSpeed Maxi Kit (one conical is its own Maxiprep). Critical...
Showing: 1 - 4 of 4 results