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We narrowed to 41 results for: his

Showing: 1 - 20 of 41 results

  1. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Trono 14666 pQE9-His-p97(wt) VCP His T5 ALS Graham Warren 14667 pQE9-His-p97(QQ) VCP His T5 ALS Graham Warren...17229 pQE9-His-p97deltaD2 VCP His T5 ALS Hemmo Meyer 17230 pQE9-His-p97deltaD2(K251A) VCP His T5 ALS Hemmo...51641 ADAM10-bio-His ADAM10 His, CD4 CMV Alzheimer's Gavin Wright 51643 APP-bio-His APP His, CD4 CMV Alzheimer's...0005 VCP His T7 ALS Axel Brunger 12339 pBD-0071 VCP His T7 ALS Axel Brunger 12344 pBD-0010 VCP His T7 ALS...0016 VCP His T7 ALS Axel Brunger 12346 pBD-0013 VCP His T7 ALS Axel Brunger 12347 pBD-0006 VCP His T7 ALS...0009 VCP His T7 ALS Axel Brunger 12349 pBD-0070 VCP His T7 ALS Axel Brunger 12350 pBD-0069 VCP His T7 ALS...0008 VCP His T7 ALS Axel Brunger 12352 pBD-0007 VCP His T7 ALS Axel Brunger 12353 pBD-0018 VCP His T7 ALS...

  2. Bacterial Expression Systems

    Type
    Collection
    ...required. pET-HIS-Sangamo 40786 TALEN Gang Bao Gateway cloning and expression of an N-terminally His-tagged ...pET-Sangamo-His 40787 TALEN Gang Bao Gateway cloning and expression of a C-terminally His-tagged TALEN...fluorescent protein variant (YPet) found in pYPet-His . pYPet-His 14031 FRET Patrick Daugherty FRET: Vector for... and his3. In bacterial strain USO hisB- pyrF- rpoZ- , this promotes growth on plates lacking His and ...Tag(s) ID Purpose pDEST-HisMBP 11085 Tac (lactose/IPTG inducible) MBP, 6x His David Waugh Empty lactose...N-terminal 6x His tag and an MBP tag. Compatible with Gateway cloning. pDEST-periHisMBP 11086 Tac (lactose... for super resolution imaging, epitope tags like His for easy western blotting or staining, and RNA transcript...

  3. Fluorescent Protein Guide: FRET

    Type
    Collection
    ... optimized CyPet pCyPet-His Cyan Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian...mammalian optimized YPet pYPet-His Yellow Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian...Cyan Bacterial Expresses Aquamarine with N-terminal His tag pAquaN1 Cyan Mammalian Expresses mammalian optimized...

  5. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Drosophila transgenesis His Epitope tag pEZYmyc-His - C-terminal Myc-His tag for mammalian expression...bacteria with N-terminal His-TrxA-TEV tag NusA Protein purification pNIC-HIS6-NUSA-TEV-GG - Cloning of...Gateway destination vector for bacterial expression; His,GST tag pE2c - Modified pENTR vector for C-terminal...the extracellular surface of S. cerevisiae cells His-tagged versions of pFastBac LIC vectors and pFastBac.... coli as TEV cleavable N-terminal His and NusA fusions Return to top Function Besides functions such ... with cap-dependent 3Myc-EYFP-HA-His6 and IRES-dependent ECFP-HA-His6 translation for mammalian expression... mammalian expression pET His6 GST TEV - N-terminal His6-GST-TEV for bacterial ...

  6. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...and cloned into an IPTG-inducible His-tag expression vector. All His-tags are cloned at the C-terminus...inexpensive to assay, and easy to purify using histidine-tagged constructs and routine protein and molecular...map, a TEV cleavage site is present between the 6xHis-tag and the coding region of MDH. For detailed clone...

  7. Biosensor AAV Preps

    Type
    Collection
    ...pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Syn CaMPARI2 his Constitutive 1 Schreiter 101061 pAAV_hsyn_NES-his-CaMPARI2...F391W-WPRE-SV40 Syn CaMPARI2-F391W his Constitutive 1 Schreiter 101064 pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40...L398T-WPRE-SV40 Syn CaMPARI2-L398T his Constitutive 1 Schreiter Calcium Sensor: jRGECO1 100852 pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40...-ChrimsonR-ST Syn jGCaMP8s none Cre-dependent 9 Histed 176749 AAV-Syn-H2B-jGCaMP8s-WPRE Syn jGCaMP8s none...

  8. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian Expression...dimerization pCEP4YPet-MAMM - Mammalian Expression pYPet-His - Bacterial Expression YPet-N1 - Mammalian Expression...Monomer pmiRFP670-N1 - Mammalian Expression pBAD/His-miRFP670 - Bacterial Expression iRFP670 643 670 13...Monomer pmiRFP703-N1 - Mammalian Expression pBAD/His-miRFP703 - Bacterial Expression iRFP702 673 702 8...Monomer pmiRFP709-N1 - Mammalian Expression pBAD/His-miRFP709 - Bacterial Expression mIFP 683 704 7 3.5...4.5 2.8 hr Dimer piRFP - Mammalian Expression pBAD/His-B-iRFP - Bacterial Expression iSplit 690 713 5 Dimer...tdTomato - Mammalian Expression pFA6a-link-tdTomato-SpHis5 - Yeast Expression tdTomato-N1 - Mammalian Expression...

  9. Institute for Protein Innovation

    Type
    Collection
    ...antibodies targeting V5, Protein C, DYKDDDDK, Rho, His, Biotin, EE, GCN4 and Strep. Please visit our Tags...

  10. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Biosensor Eric Schreiter AV-1-PV4704 101064-AAV1 pAAV_hsyn_NES-his-CaMPARI2... Schreiter AV-1-PV4705 101061-AAV1 pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40 Biosensor Eric Schreiter...

  11. TALEN Guide

    Type
    Collection
    ...21822235 . See the Q&A with Dr. Adam Bogdanove and his thoughts on the TALe technology here ....already become the best-selling kit in Addgene’s history. Dr. Keith Joung’s lab at Massachusetts General...

  13. Cre-lox system

    Type
    Collection
    ...Mammalian Sato 123128 pET-His-Cre 6xHis-Cre T7 Bacterial Liu 123131 pET-His-VCre 6xHis-VCre T7 Bacterial Liu...9xGGS-Cre-6xHis Cre fused to highly cationic GFP variant T7 Bacterial Liu 62939 pET-Cre-6xHis Cre-6xHis T7 Bacterial...Nxph4 Mammalian Zeng 62372 pET-(-30)GFP-9xGGS-Cre-6xHis Cre fused to supernegatively charged GFP variant...; Targeting vector for Pax7 Tanaka 111187 pBADZ-HisCre Cre; arabinose inducible. PBAD promoter Bacterial...

  14. Antibody Guide

    Type
    Collection
    ..., or by using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs can have weak signals...location. Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting...in these assays. This method is suitable for DNA:histone modifier interactions, which have strong binding...methods such as immunofluorescence (IF), immunohistochemistry (IHC), and immunocytochemistry (ICC) use... they only have one protein of interest. Immunohistochemistry (IHC) and immunocytochemistry (ICC) IF can... can refer to both immunohistochemistry and immunocytochemistry when fluorescent signaling molecules (...

  15. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...p15TV-L EF456736 Hexahistidine tag with TEV cleavage, AmpR 26093 pET15-MHL EF456738 Hexahistidine tag with TEV...GST-tag and hexahistidine tag with Thrombin cleavage, KanR 26096 pET28-MHL EF456735 Hexahistidine tag with...26117 pNIC-CH EF199843 Hexahistidine tag, AmpR 26103 pNIC28-Bsa4 EF198106 Hexahistidine tag with TEV cleavage...EF199844 Hexahistidine tag, FLAG tag with TEV cleavage, KanR 26106 pNH-TrxT GU269914 Hexahistidine tag, thioredoxin...GU452710 Hexahistidine tag , Z-basic, TEV cleavage, AmpR 26108 pFB-LIC-Bse EF199842 Hexahistidine tag with...TEV cleavage, AmpR 26094 pET28a-LIC EF442785 Hexahistidine tag with Thrombin cleavage, KanR 26101 pET28GST-LIC...ptac promoter, AmpR 26099 pFBOH-LIC EF456740 Hexahistidine tag with Thrombin cleavage, Bac-to-Bac Baculovirus...

  16. Genetic Code Expansion

    Type
    Collection
    ...PylRS M. barkeri azidolysine (AzK) Mammalian TAG Abhishek Chatterjee 217364 pAcBac1-BacMam-4x-PyOtR PyOtR...PyOtR M. mazei azidolysine (AzK) Mammalian TAG Abhishek Chatterjee 218764 pEvoltac-EcW-TGA-h14 TrpRS-h14...TrpRS-h14 (S8A, V144G, V146C) E. coli Bacterial TGA Abhishek Chatterjee 218765 pEvoltac EcW-TGA-h9 TrpRS-h9 (... (S8A, V144S, V146A) E. coli Bacterial TGA Abhishek Chatterjee 218766 pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA...D182S, F183M, L186A, D265R) E. coli Bacterial TAG Abhishek Chatterjee 218767 pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA...D182C, F183Y, L186C, D265R) E. coli Bacterial TAG Abhishek Chatterjee 220800 EF1a-L274GMmMetRS-T2A-mCherry...incorporate other Uaas (derived from EcNR1 strain) Abhishek Chatterjee 218769 ATMW-BL21 Removed endogenous...

  17. Immunology Research Plasmids and Resources

    Type
    Collection
    ...-A major histocompatibility complex, class I, A FLJ26655, HLAA HLA-B major histocompatibility complex,...major histocompatibility complex, class II, DM beta D6S221E, RING7 HLA-DOA major histocompatibility complex...HLA-DOB major histocompatibility complex, class II, DO beta DOB HLA-DPA1 major histocompatibility complex, ...major histocompatibility complex, class II, DQ alpha 2 HLA-DXA HLA-DQB1 major histocompatibility complex...major histocompatibility complex, class II, DR alpha HLA-DRA1 HLA-DRB1 major histocompatibility complex... HLA-G major histocompatibility complex, class I, G MHC-G HLA-H major histocompatibility complex, class...protein-coupled receptor 77 C5L2, GPF77 HTN3 histatin 3 HIS2, HTN2, HTN5 IL8 interleukin 8 CXCL8, GCP-1,...

  18. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...206528 Anti-Histone H3 pThr11 [N123/19R] Histone H3 pThr11 Rat Mouse IgG2a 206529 Anti-Histone H3 pThr11...28R] HCN3 Mouse Mouse IgG2a 177500 Anti-6xHis [N144/14R] 6xHis Mouse IgG2a 177501 Anti-TrpV3 [N15/4R] ...Pan-Gamma-protocadherin-A Mouse IgG2a 182106 Anti-Histone H3.3 [N158/28R] Histone H3.3 Rat Mouse IgG2a 182107 Anti-LRRTM2...Mouse Mouse IgG2a 206552 Anti-Histone H4-dimethyl-Arg3 [N309A/21R] Histone H4-dimethyl-Arg3 Human Mouse...musculus (mouse) IgG2b 227037 6xHis recombinant mouse monoclonal antibody. 6xHis Chimera: M. musculus (mouse... Mouse IgG2a 188214 Anti-GST [N100/13R ] GST Schistosoma japonicum Mouse IgG2a 188215 Anti-TrpC7 [N64A...pThr11 [N123/48R] Histone H3 pThr11 Rat Mouse IgG2a 206530 Anti-Haspin/GSG2 [N128A/2R] Haspin/GSG2 Human Mouse...

  19. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...Available modifications include: histone acetylation by p300 histone demethylation by LSD1 cytosine methylation...for targeted epigenetic modification, including histone acetylation/demethylation, and cytosine methylation...
Showing: 1 - 20 of 41 results