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pBN078

Plasmid

#167959

Purpose

express E. coli E69 (O9a:K30) Wzc with Y717E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y717E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y717E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pME18s-Ix_NPYLR1b

Plasmid

#138748

Purpose

Expresses Ixodes scapularis NPY-like receptor 1b (NPYLR1b) in a mammalian expression vector

Depositor

Leslie Vosshall

Article

Liesch et al PLoS Negl Trop Dis. 2013 Oct 10;7(10):e2

Insert

Ixodes scapularis NPYLR1b (LOC8025400 Ixodes scapularis)

Expression

Mammalian

Promoter

SRa

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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CAG-loxP-3xpA-H2BsfGFP-tdTomato - linker E

Plasmid

#125797

Purpose

Histone H2B-superfolder GFP and tdTomato fused by unskippable linker

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

H2BsfGFP-tdTomato

Use

Cre/Lox

Expression

Mammalian

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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YCe3766 HC_Kan_3'-LTR_p10

Plasmid

#100703

Purpose

part designed to occupy position 10 of EMMA. Functional category: Long Terminal Repeat

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

Lentiviral 3' LTR

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe3777 HC_Kan_Chimeric-5'LTR_p1

Plasmid

#100704

Purpose

part designed to occupy position 1 of EMMA. Functional category: Long Terminal Repeat

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

Lentiviral chimeric 5' LTR

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1889 HC_Kan_SV40-polyA_p11

Plasmid

#100678

Purpose

part designed to occupy position 11 of EMMA. Functional category: polyadenylation signal sequence

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

SV40-polyA

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1895 HC_Kan_Kozak-ATG_p6

Plasmid

#100680

Purpose

part designed to occupy position 6 of EMMA. Functional category: Kozak sequence

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

Kozak-ATG

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2026 HC_Kan_mRuby2_p9

Plasmid

#100687

Purpose

part designed to occupy position 9 of EMMA. Functional category: Fluorescent marker

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

mRuby2

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2030 HC_Kan_bGHpA_p16

Plasmid

#100688

Purpose

part designed to occupy position 16 of EMMA. Functional category: polyadenylation signal sequence

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

bGHpA

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2034 HC_Kan_mTagBFP2_p9

Plasmid

#100689

Purpose

part designed to occupy position 9 of EMMA. Functional category: Fluorescent marker

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

mTAGBFP2

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2060 HC_Kan_Linker3_p20

Plasmid

#100695

Purpose

part designed to occupy position 20 of EMMA. Functional category: Peptide linker

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

Linker3

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2746 HC_Kan_Linker1_p8

Plasmid

#100699

Purpose

part designed to occupy position 8 of EMMA. Functional category: Peptide linker

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

Linker1

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2748 HC_Kan_5'ITR-PB_p1

Plasmid

#100700

Purpose

part designed to occupy position 1 of EMMA. Functional category: Inverted Terminal Repeat

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

Piggybac 5' ITR

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2750 HC_Kan_3'ITR-PB_p24

Plasmid

#100701

Purpose

part designed to occupy position 24 of EMMA. Functional category: Inverted Terminal Repeat

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

Piggybac 3' ITR

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe4520 HC_Kan_conn_BsaI-1_p1

Plasmid

#100668

Purpose

EMMA toolkit Assembly connector for second step assembly

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EMMA toolkit Assembly connector for second step assembly

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2725 HC_Kan_conn_BsaI-2_p24

Plasmid

#100669

Purpose

EMMA toolkit Assembly connector for second step assembly

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EMMA toolkit Assembly connector for second step assembly

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe4522 HC_Kan_conn_BsaI-3_p1

Plasmid

#100670

Purpose

EMMA toolkit Assembly connector for second step assembly

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EMMA toolkit Assembly connector for second step assembly

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2730 HC_Kan_conn_BsaI-4_p25

Plasmid

#100671

Purpose

EMMA toolkit Assembly connector for second step assembly

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EMMA toolkit Assembly connector for second step assembly

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1759 HC_Kan_CAGp_p3

Plasmid

#100672

Purpose

part designed to occupy position 3 of EMMA. Functional category: Constitutional promoter

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

CAG promoter

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pFusX4-41

Plasmid

#63549

Purpose

Encodes RVD positions 10-12 in TALEN assembly

Depositor

Stephen Ekker

Article

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

NN NN NI

Use

TALEN

Mutation

none

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only