We narrowed to 17,732 results for: SHE

1. pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine

   Plasmid

   #50472

   Purpose

   Gs-coupled rM3D-IRES-mCitrine under the control of GFAP promoter

   Depositor

   Bryan Roth

   Has Service

   AAV5

   Insert

   rM3D-IRES-mCitrine (CHRM3 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   GFAP

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. PRDX6A-c001

   Plasmid

   #175474

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.

   Depositor

   Opher Gileadi

   Insert

   PRDX6 (PRDX6 Human)
   

   Use

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Mutation

   See Depositor Comments

   Promoter

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. p-dCas9-TET1-Hygro

   Plasmid

   #104404

   Purpose

   transient expression of dCas9-TET1 fusion protein

   Depositor

   Stephan Beck

   Insert

   TET1 (TET1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETNb-cALFA

   Plasmid

   #159989

   Purpose

   For the protein expression with ALFA tag at C-terminus in E. coli

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Use

   Tags

   HRV3C protease cleavage site-ALFA tag-8histidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. HECTD1

   Plasmid

   #32860

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   HECTD1 (HECTD1 Human)
   

   Use

   Tags

   His (with TEV cleavage)

   Expression

   Bacterial

   Mutation

   amino acids 1879-1966 only

   Promoter

   Available since

   Jan. 6, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pelBHisTEV

   Plasmid

   #157741

   Purpose

   empty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sites

   Depositor

   Debra Hansen

   Type

   Empty backbone

   Use

   Tags

   PelB signal sequence + His10-tag + TEV protease c…

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. AICSDP-39:RAB5A-mEGFP

   Plasmid

   #107579

   Purpose

   Homology arms and mEGFP-linker sequence for N-terminus tagging of human RAB5A

   Depositor

   Allen Institute for Cell Science

   Insert

   RAB5A Homology Arms with mEGFP-linker (RAB5A Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   May 9, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. TC1541A

   Plasmid

   #164875

   Purpose

   Split GFP reporter expressing GFP(beta1-10) following C>U editing of the target site

   Depositor

   Tian Chi

   Article

   Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

   Insert

   Split GFP(beta1-10)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTmpKm

   Plasmid

   #176227

   Purpose

   A template plasmid for amplification of the cI-hok-neo cassette

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   cI of phage lambda, hok of the R1 plasmid, neo
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmTurquoise2-Drp1

    Plasmid

    #110946

    Purpose

    Expresses dynamin-like protein 1 fused to mTurquoise2

    Depositor

    Pablo Rivera-Fuentes

    Insert

    Dynamin-1-like protein (DNM1L Human)
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. MECP2 in pENTR

    Plasmid

    #216592

    Purpose

    gateway cloning donor vector containing MECP2

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

    Plasmid

    #159908

    Purpose

    Mutagenesis of Htr2a

    Depositor

    Larry Zweifel

    Insert

    Htr2a (Htr2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3-FLAG-SENP3

    Plasmid

    #115521

    Purpose

    Mammalian expression construct

    Depositor

    Martine Roussel

    Article

    Kuo et al Cell Cycle. 2008 Nov 1;7(21):3378-87. do

    Insert

    SENP3 (Senp3 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. DYRK1A

    Plasmid

    #38913

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    DYRK1A (DYRK1A Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pGFP Cas

    Plasmid

    #50729

    Purpose

    mammalian expression of GFP-Cas. Note-This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Insert

    Cas (Bcar1 Rat)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. Flag FoxO3

    Plasmid

    #14937

    Depositor

    Joan Massague

    Article

    Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

    Insert

    FoxO3 (FOXO3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid

    #50470

    Purpose

    Gq-coupled hM3D-IRES-mCitrine under the control of GFAP promoter

    Depositor

    Bryan Roth

    Insert

    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    GFAP

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB-CAG-Dest-pA-pgk-bsd

    Plasmid

    #74918

    Purpose

    Gateway destination piggyBac vector

    Depositor

    Jose Silva

    Article

    dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pJCX3-ACTG2_R257C

    Plasmid

    #228911

    Purpose

    Expresses mutant R257C smooth muscle actin gamma-2 in human cells

    Depositor

    Roberto Dominguez

    Insert

    Actin gamma 2, smooth muscle (ACTG2 Human)
    

    Use

    Tags

    HIS-FLAG-TEV

    Expression

    Bacterial and Mammalian

    Mutation

    R257C

    Promoter

    CMV

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJCX3-ACTG2_WT

    Plasmid

    #228910

    Purpose

    Expresses wildtype smooth muscle actin gamma-2 in human cells

    Depositor

    Roberto Dominguez

    Insert

    Actin gamma 2, smooth muscle (ACTG2 Human)
    

    Use

    Tags

    HIS-FLAG-TEV

    Expression

    Bacterial and Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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