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iSPI_pSer_Full_library

Pooled Library

#188536

Purpose

Express a library of MBP-Fusion proteins containing phosphoserine at a defined position in the fusion protein

Depositor

Jesse Rinehart

Article

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Expression

Bacterial

Species

Homo sapiens

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only
pHTC-HaloTag_BOP1:1-746

Plasmid

#211005

Purpose

Mammalian expression of full-length humanBOP1. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

BOP1:M1-T746 (BOP1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTN-HaloTag_BOP1:1-746

Plasmid

#211004

Purpose

Mammalian expression of full-length human BOP1. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

BOP1:M1-T746 (BOP1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTN-HaloTag_DCAF1:1-1507

Plasmid

#210972

Purpose

Mammalian expression of full-length human DCAF1. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF1:M1-E1507 (DCAF1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTC-HaloTag_DCAF1:1-1507

Plasmid

#210973

Purpose

Mammalian expression of full-length human DCAF1. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF1:M1-E1507 (DCAF1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pNLF1-C_DCAF1:1-1507

Plasmid

#210950

Purpose

Mammalian expression of full-length human DCAF1. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF1:M1-E1507 (DCAF1 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_DCAF1:1-1507

Plasmid

#210945

Purpose

Mammalian expression of full-length human DCAF1. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF1:M1-E1507 (DCAF1 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_DAW1:1-415

Plasmid

#211237

Purpose

Mammalian expression of full-length human DAW1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DAW1:M1-R415 (DAW1 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_DCAF11:1-546

Plasmid

#210907

Purpose

Insect expression of full-length human DCAF11. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF11:M1-Q546 (DCAF11 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

R207H natural variant (VAR_020121)

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-LIC_DDB1:1-1140

Plasmid

#210886

Purpose

Insect expression of full-length human DDB1. Untagged.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DDB1:M1-H1140 (DDB1 Human)

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-LIC_GRWD1:1-446

Plasmid

#210876

Purpose

Insect expression of full-length human GRWD1. N-terminal 6X His tag with thrombin protease cleavage site.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

GRWD1:M1-V446 (GRWD1 Human)

Tags

MGSSHHHHHHSSGLVPRGS

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBiT3.1-N_DCAF7:1-342

Plasmid

#211118

Purpose

Mammalian expression of full-length human DCAF7. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF7:M1-V342 (DCAF7 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_WDR62:1-1518

Plasmid

#211257

Purpose

Mammalian expression of full-length human WDR62 isoform 4. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR62:M1-H1518 (WDR62 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

isoform 4. L850S natural VAR_031299. Q1305L n…

Promoter

CMV

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pHTC-HaloTag_RAB7A:1-207

Plasmid

#211096

Purpose

Mammalian expression of full-length human RAB7A. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RAB7A:M1-C207 (RAB7A Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pNLF1-C_PLCG1:1-1291

Plasmid

#211089

Purpose

Mammalian expression of full-length human PLCG1 isoform 2. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

PLCG1:M1-L1291 (PLCG1 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

isoform 2. I813T natural variant (VAR_011908)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_SCAP:1-905

Plasmid

#211228

Purpose

Mammalian expression of full-length human SCAP isoform 2. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SCAP:M1-D905 (SCAP Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

isoform 2. V798I natural variant (VAR_012203)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_SCAP:1-905

Plasmid

#211302

Purpose

Mammalian expression of full-length human SCAP isoform 2. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SCAP:M1-D905 (SCAP Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

isoform 2. V798I natural variant (VAR_012203)

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_DCAF11:1-546

Plasmid

#211197

Purpose

Mammalian expression of full-length human DCAF11. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF11:M1-Q546 (DCAF11 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

R207H natural variant (VAR_020121)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-C_CBFA2T3:1-615

Plasmid

#211070

Purpose

Mammalian expression of full-length human CBFA2T3. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CBFA2T3:M1-R615 (CBFA2T3 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

not annotated isoform

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-C_PRKCSH:1-527

Plasmid

#211091

Purpose

Mammalian expression of full-length human PRKCSH. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

PRKCSH:M1-L527 (PRKCSH Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

333-333: missing. A291T natural variant (VAR_028…

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge