We narrowed to 1,719 results for: plasmid for e coli

1. pE-SUMO CRYGS

   Plasmid

   #80753

   Purpose

   Expression of human gamma S-crystallin in E. coli

   Depositor

   Larry David

   Insert

   CRYGS (CRYGS Human)
   

   Use

   Tags

   6xHIS and Smt3 SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a_LYN

   Plasmid

   #224238

   Purpose

   Expression of human LYN Kinase in E. coli

   Depositor

   Andrew Mesecar

   Insert

   lyn (LYN Human)
   

   Use

   Tags

   6xHis and 6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSMH1741

   Plasmid

   #160930

   Purpose

   Expression of soluble Sc octamer (H3)

   Depositor

   Stephen Harrison

   Inserts

   HHF1
   HTA1
   HTB1
   HHT1
   

   Use

   Tags

   6His-TEV, N-6His-TEV, N-terminal 6His-TEV, and no…

   Expression

   Mutation

   codon-optimized for E. coli -- see provided seque…

   Promoter

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSMH1742

   Plasmid

   #160929

   Purpose

   Expression of soluble Sc octamer (Cse4)

   Depositor

   Stephen Harrison

   Inserts

   HHF1
   HTA1
   HTB1
   Cse4
   

   Use

   Tags

   6His-TEV, N-terminal 6His-TEV, and none

   Expression

   Mutation

   codon-optimized for E. coli -- see provided seque…

   Promoter

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTCF176 HLA-A*02:01 (CD8 mut)

   Plasmid

   #180459

   Purpose

   E. coli expression of soluble MHC protein

   Depositor

   John Altman

   Insert

   HLA-A*02:01 (CD8 mut)
   

   Use

   Tags

   BSP41

   Expression

   Bacterial

   Mutation

   D227K, T228A, replacement of transmembrane domain…

   Promoter

   Available Since

   Feb. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PAS_RvSAHS6

   Plasmid

   #215647

   Purpose

   Expresses SUMO-tagged RvSAHS6 protein from tardigrade R. varieornatus in E. coli

   Depositor

   Pamela Silver

   Insert

   SAHS6
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PAS_RvSAHS1

   Plasmid

   #215645

   Purpose

   Expresses SUMO-tagged RvSAHS1 protein from tardigrade R. varieornatus in E. coli

   Depositor

   Pamela Silver

   Insert

   SAHS1
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX-4T_CIP2A_1-560

   Plasmid

   #217921

   Purpose

   E. coli expression of GST-tagged CIP2A_1-560 protein

   Depositor

   Jukka Westermarck

   Insert

   CIP2A (CIP2A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTCF79 H-2D(b) (CD8 mut)

   Plasmid

   #180453

   Purpose

   E. coli expression of soluble MHC protein

   Depositor

   John Altman

   Insert

   H-2D(b) (CD8 mut)
   

   Use

   Tags

   BSP41

   Expression

   Bacterial

   Mutation

   D227K, replacement of transmembrane domain with e…

   Promoter

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pE-SUMO CRYBA3

    Plasmid

    #80747

    Purpose

    Expression of human beta A3-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYBA3 (CRYBA1 Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUC6

    Plasmid

    #49793

    Purpose

    E. coli cloning vector

    Depositor

    Joachim Messing

    Article

    Vieira et al Gene. 1982 Oct;19(3):259-68.

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pE-SUMO CRYBA1

    Plasmid

    #80745

    Purpose

    Expression of human beta A1-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYBA1 (CRYBA1 Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Sc hk/P-RFA3-pCDFDuet

    Plasmid

    #240743

    Purpose

    Over-express his(10)-PKA kinase motif-PreScission protease site-Sc RFA3 in E. coli

    Depositor

    Michael O'Donnell

    Insert

    hk/P-RFA3 (RFA3 Budding Yeast)
    

    Use

    Tags

    His(10) followed by PKA kinase motif follwed by P…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a_HCK

    Plasmid

    #224239

    Purpose

    Expression of human HCK Kinase in E. coli

    Depositor

    Andrew Mesecar

    Insert

    hck (HCK Human)
    

    Use

    Tags

    6xHis and 6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PAS_HeSAHS4

    Plasmid

    #215648

    Purpose

    Expresses SUMO-tagged HeSAHS4 protein from tardigrade H. exemplaris in E. coli

    Depositor

    Pamela Silver

    Insert

    SAHS4
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PAS_RvSAHS4

    Plasmid

    #215646

    Purpose

    Expresses SUMO-tagged RvSAHS4 protein from tardigrade R. varieornatus in E. coli

    Depositor

    Pamela Silver

    Insert

    SAHS4
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJA12 Mamu-A1*001:01

    Plasmid

    #180442

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    Mamu-A1*001:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJA13 Mamu-A1*002:01

    Plasmid

    #180443

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    Mamu-A1*002:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTCF122 Mamu-B*008:01

    Plasmid

    #180455

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    Mamu-B*008:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available Since

    Feb. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pK27Sumo_His-SUMO-PLpro (nsp3) (SARS-CoV-2)

    Plasmid

    #169193

    Purpose

    To express SARS-CoV-2 Papain-like protease in E. coli

    Depositor

    John Diffley

    Article

    Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

    Insert

    14His-SUMO-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    14His-SUMO

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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