We narrowed to 10,125 results for: Uty

1. pHAGE-Gfi1b-mCh

   Plasmid

   #193065

   Purpose

   constitutive expression of mouse Gfi1b fused to mCherry in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

   Insert

   Gfi1b-mCh
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV_tKiMBImut-T2A-caMEK

   Plasmid

   #199579

   Purpose

   Express tKiMBImut(AA) and caMEK in an AAV vector

   Depositor

   Michael Lin

   Article

   Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

   Inserts

   ERK tdTomato-Kinase-Modulated Bioluminescent Indicator (mutant)
   constitutively active MEK
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBT9-GGA2-GAE

   Plasmid

   #199175

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-GGA2-GAE domain fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

   Insert

   GGA2-GAE domain (GGA2 Human)
   

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Contains Lys 545 Gln substitution

   Promoter

   ADH1

   Available Since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-A10

   Plasmid

   #196075

   Purpose

   Constitutive CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Insert

   gRNA targeting Adam10
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Cloned using SapI sites - destroyed after inserti…

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-A10

   Plasmid

   #196077

   Purpose

   Constitutive CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Insert

   gRNA targeting Adam10
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Cloned using SapI sites - destroyed after inserti…

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pS238D1::tse5-CT

   Plasmid

   #192958

   Purpose

   For expression of Tse5-CT (1169-1317) in P. putida for biological function studies

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   tse5-CT
   

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1317)

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV puro PRDM14 ΔSET domain

   Plasmid

   #193680

   Purpose

   Constitutive retroviral expression of PRDM14

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   PRDM14 (PRDM14 Human)
   

   Use

   Retroviral

   Mutation

   Deletion of SET domain

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV puro PRDM14 ΔZinc finger domain

   Plasmid

   #193679

   Purpose

   Constitutive retroviral expression of PRDM14

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   PRDM14 (PRDM14 Human)
   

   Use

   Retroviral

   Mutation

   Deletion of Zinc finger domain

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBeast-pT7-lox

   Plasmid

   #190059

   Purpose

   Express the bacterial lactate oxidase enzyme under the control of the strong constitutif phage promoter T7

   Depositor

   Jerome Bonnet

   Article

   Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

   Insert

   lox
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBeast-pT7-codA

    Plasmid

    #190058

    Purpose

    Express the bacterial choline oxidase enzyme under the control of the strong constitutif phage promoter T7

    Depositor

    Jerome Bonnet

    Article

    Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

    Insert

    codA
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSCL.178

    Plasmid

    #184999

    Purpose

    Express -Eco1 FANCF editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: FANCF targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    FANCF donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSCL.176

    Plasmid

    #184997

    Purpose

    Express -Eco1 RNF2 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: RNF2 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    RNF2 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSCL.179

    Plasmid

    #185000

    Purpose

    Express -Eco1 HEK4 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: HEK4 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    HEK4 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmRE-Tn5-142

    Plasmid

    #118531

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mClover3
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCEP4-MR1 (M50P)

    Plasmid

    #178651

    Purpose

    Mammalian expression of myc-tagged MR1 (M50P)

    Depositor

    Erik Procko

    Article

    McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

    Insert

    MR1 (MR1 Human)
    

    Tags

    Extracellular c-myc epitope ta and Signal peptide…

    Expression

    Mammalian

    Mutation

    M50P

    Promoter

    CMV

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEP4 MR1 (H83P)

    Plasmid

    #178652

    Purpose

    Mammalian expression of myc-tagged MR1 (H83P)

    Depositor

    Erik Procko

    Article

    McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

    Insert

    MR1 (MR1 Human)
    

    Tags

    Extracellular c-myc epitope ta and Signal peptide…

    Expression

    Mammalian

    Mutation

    H83P

    Promoter

    CMV

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCEP4 MR1 (Q153P)

    Plasmid

    #178653

    Purpose

    Mammalian expression of myc-tagged MR1 (Q153P)

    Depositor

    Erik Procko

    Article

    McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

    Insert

    MR1 (MR1 Human)
    

    Tags

    Extracellular c-myc epitope ta and Signal peptide…

    Expression

    Mammalian

    Mutation

    Q153P

    Promoter

    CMV

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTS2333_Tier3(Lenti_inverse)-YPet_Puro

    Plasmid

    #169664

    Purpose

    Tier-3 vector for stable integration via lentiviral transduction in inverted direction with polyA carrying a constitutive expression cassette for puromyine and Ypet (PCMV-5'LTR-Psi-gag-env-PRPBSA-YPet-p2A-PuroR-pA::A2-pA::A3-pA-WPRE-3'LTR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven YPet and PuroR expression
    

    Expression

    Mammalian

    Promoter

    PhCMV / RPBSA

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDD2604

    Plasmid

    #175088

    Purpose

    Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T604A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

    Insert

    KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
    

    Expression

    Mammalian

    Mutation

    S644A

    Available Since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDD2602

    Plasmid

    #175086

    Purpose

    Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T424A, T438A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

    Insert

    KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
    

    Expression

    Mammalian

    Mutation

    T424A, T438A

    Available Since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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