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pDONR223-GOLPH3

Plasmid

#21688

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Gateway entry clone

Mutation

last nucleotide of stop codon removed for c-termi…

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
GCK gRNA (BRDN0001144982)

Plasmid

#77581

Purpose

3rd generation lentiviral gRNA plasmid targeting human GCK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GCK (GCK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SIN-cPPT-H1-sgHtt1-7SK-sgCas9-PGK-mCherry-WPRE

Plasmid

#87912

Purpose

Kamicas9 lentiviral vector

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

shHTT1, sgCas9, cherry

Use

Lentiviral

Promoter

H1, 7SK, PGK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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LUC7L

Plasmid

#155713

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

LUC7L (LUC7L Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 18, 2022

Availability

Academic Institutions and Nonprofits only

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LYN gRNA (BRDN0001145823)

Plasmid

#77028

Purpose

3rd generation lentiviral gRNA plasmid targeting human LYN

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LYN (LYN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCDB181

Plasmid

#80692

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEHEE_06

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEHEE_06

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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CLK2 gRNA (BRDN0001148263)

Plasmid

#77604

Purpose

3rd generation lentiviral gRNA plasmid targeting human CLK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CLK2 (CLK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CLK2 gRNA (BRDN0001145900)

Plasmid

#77607

Purpose

3rd generation lentiviral gRNA plasmid targeting human CLK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CLK2 (CLK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ACVR1 gRNA (BRDN0001145448)

Plasmid

#76757

Purpose

3rd generation lentiviral gRNA plasmid targeting human ACVR1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ACVR1 (ACVR1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMCSF-R(mA)-luc

Plasmid

#12421

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

Insert

M-CSF receptor promoter fragment (CSF1R Human)

Use

Luciferase

Mutation

C/EBP binding site mutation (created a KpnI site …

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
GNE gRNA (BRDN0001145959)

Plasmid

#77832

Purpose

3rd generation lentiviral gRNA plasmid targeting human GNE

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GNE (GNE Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GNE gRNA (BRDN0001145858)

Plasmid

#77830

Purpose

3rd generation lentiviral gRNA plasmid targeting human GNE

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GNE (GNE Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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ILK gRNA (BRDN0001147357)

Plasmid

#75909

Purpose

3rd generation lentiviral gRNA plasmid targeting human ILK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ILK (ILK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ILK gRNA (BRDN0001146314)

Plasmid

#75910

Purpose

3rd generation lentiviral gRNA plasmid targeting human ILK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ILK (ILK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ILK gRNA (BRDN0001144869)

Plasmid

#75911

Purpose

3rd generation lentiviral gRNA plasmid targeting human ILK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ILK (ILK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CAMK2B gRNA (BRDN0001145558)

Plasmid

#77743

Purpose

3rd generation lentiviral gRNA plasmid targeting human CAMK2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CAMK2B (CAMK2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CAMK2B gRNA (BRDN0001148038)

Plasmid

#77744

Purpose

3rd generation lentiviral gRNA plasmid targeting human CAMK2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CAMK2B (CAMK2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CAMK2G gRNA (BRDN0001148688)

Plasmid

#76237

Purpose

3rd generation lentiviral gRNA plasmid targeting human CAMK2G

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CAMK2G (CAMK2G Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CAMK2G gRNA (BRDN0001148569)

Plasmid

#76238

Purpose

3rd generation lentiviral gRNA plasmid targeting human CAMK2G

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CAMK2G (CAMK2G Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BRD2 gRNA (BRDN0001145548)

Plasmid

#77986

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRD2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRD2 (BRD2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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