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pX459-Adam10 gRNA

Plasmid

#246568

Purpose

CRISPR vector co-expressing Cas9 and a mouse Adam10 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Adam10 gRNA (Adam10 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Tmem2 gRNA

Plasmid

#246569

Purpose

CRISPR vector co-expressing Cas9 and a mouse Tmem2 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Tmem2 gRNA (Cemip2 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Arf1 gRNA

Plasmid

#246572

Purpose

CRISPR vector co-expressing Cas9 and a mouse Arf1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Arf1 gRNA (Arf1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Fzd4 gRNA

Plasmid

#246563

Purpose

CRISPR vector co-expressing Cas9 and a mouse Fzd4 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Fzd4 gRNA (Fzd4 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Itga5 gRNA

Plasmid

#246573

Purpose

CRISPR vector co-expressing Cas9 and a mouse Itga5 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Itga5 gRNA (Itga5 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Fzd5 gRNA

Plasmid

#246564

Purpose

CRISPR vector co-expressing Cas9 and a mouse Fzd5 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Fzd5 gRNA (Fzd5 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Fzd8 gRNA

Plasmid

#246565

Purpose

CRISPR vector co-expressing Cas9 and a mouse Fzd8 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Fzd8 gRNA (Fzd8 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Fzd3 gRNA

Plasmid

#246552

Purpose

CRISPR vector co-expressing Cas9 and a mouse Fzd3 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Fzd3 gRNA (Fzd3 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_amhc(myh6)_cmlc2-nmKate

Plasmid

#238375

Purpose

Drives expression of 3 different gRNAs targeting amhc (myh6), and expression of nclear mKate in cardiomyocytes.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting amhc

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-cmlc2-nGFP-nCas9

Plasmid

#238308

Purpose

Expression of nuclear GFP and nuclear Cas9 specifically in cardiomyocytes driven by the cmlc2(myl7) promoter.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

Cas9 (Csn1) endonuclease from Streptococcus pyogenes

Use

Tol2-based plasmid

Tags

nGFP-P2A

Promoter

cmlc2 (myl7)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2809

Plasmid

#232510

Purpose

Expression plasmid of Cys-light LetA(L94C,I383C) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al bioRxiv 2025.03.21.644421

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

[6xHis2xQH-TEV-LetA1(1-223,L94C,C124S)]-[LetA2(22…

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2820

Plasmid

#232511

Purpose

Expression plasmid of Cys-light LetA(Q180C,R380C) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al bioRxiv 2025.03.21.644421

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

[6xHis2xQH-TEV-LetA1(1-223,Q180C,C124S)]-[LetA2(2…

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1261

Plasmid

#235726

Purpose

Protein expression of His6-SUMO-human RAB5A Q79L 1-211 in bacteria

Depositor

Roger Williams

Article

Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

Insert

RAB5A (RAB5A Human)

Tags

6xHis-SUMO-TEV

Expression

Bacterial

Mutation

Q79L, aa 1-211

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Cd109 gRNA

Plasmid

#246571

Purpose

CRISPR vector co-expressing Cas9 and a mouse Cd109 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Cd109 gRNA (Cd109 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Fzd9 gRNA

Plasmid

#246551

Purpose

CRISPR vector co-expressing Cas9 and a mouse Fzd9 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Fzd9 gRNA (Fzd9 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Itgav gRNA

Plasmid

#246576

Purpose

CRISPR vector co-expressing Cas9 and a mouse Itgav gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Itgav gRNA (Itgav Mouse)

Use

CRISPR

Promoter

U6

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAcGP67A-mTLR4ecd-VLR

Plasmid

#187877

Purpose

Baculovirus protein expression of mouse TLR4 (residues 26–544) fused with hagfish variable lymphocyte receptor (VLR) (residues 126–200)

Depositor

Bruce Beutler

Article

Wang et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

TLR4ecd (Tlr4 Mouse)

Expression

Insect

Mutation

includes residues 26–544 of mouse TLR4

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-CMV-CNOT3-E95A-Tev-3xFlag

Plasmid

#245726

Purpose

Express CNOT3-E95A-Tev-3xFlag in mammalian cells

Depositor

Joshua Mendell

Article

Zhu et al Science. 2024 Nov 22;386(6724):eadq8587.

Insert

CNOT3-E95A-Tev-3xFlag (CNOT3 Human)

Use

Lentiviral

Tags

3xFlag

Mutation

E95A

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-CMV-CNOT3-K105S-Tev-3xFlag

Plasmid

#245727

Purpose

Express CNOT3-K105S-Tev-3xFlag in mammalian cells

Depositor

Joshua Mendell

Article

Zhu et al Science. 2024 Nov 22;386(6724):eadq8587.

Insert

CNOT3-K105S-Tev-3xFlag (CNOT3 Human)

Use

Lentiviral

Tags

3xFlag

Mutation

K105S

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-CMV-CNOT3-R59S-Tev-3xFlag

Plasmid

#245725

Purpose

Express CNOT3-R59S-Tev-3xFlag in mammalian cells

Depositor

Joshua Mendell

Article

Zhu et al Science. 2024 Nov 22;386(6724):eadq8587.

Insert

CNOT3-R59S-Tev-3xFlag (CNOT3 Human)

Use

Lentiviral

Tags

3xFlag

Mutation

R59S

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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