...viral service items we employ dozens of pieces of energy-intensive lab equipment on a daily basis. By the...allows a community like Watertown to bulk purchase energy from a higher percentage of renewable sources. ... drawing 100% of our electricity from renewable energy projects right here in New England- the majority...ventilation, and lab lighting/HVAC requires a significant energy expenditure here at Addgene. These are critical... or substantially reduced in an attempt to save energy. The transition to 100% renewable electricity will... will allow us to maintain these particularly energy-intensive systems in a more environmentally responsible...

...scientists I’ve met began doing research in their undergraduate years. We each have a unique “origin story,”... provide some perspective and advice to any undergraduate interested in getting a taste of the role of...Professor Sometimes teaches graduate and/or undergraduate classes Postdoctoral researcher (postdoc...choosing one as their official thesis lab Undergraduate student Roles and responsibilities can vary...would also highly recommend talking to other undergraduate researchers, as well as graduate students (your...’s something you want to dedicate your time and energy to. How can I make the most of my experience in...valuable for anyone in the lab though, including undergrads. I found a lot of career resources on Science...

... in their careers? A CURE for Biochemistry Undergraduate biochemistry courses are often associated with... what is now commonly termed a Course-based Undergraduate Research Experience (CURE). Having incorporated...Knutson et al., 2010a,b).      Fig. 1: Undergraduates in Provost’s CURE prepare to analyze their ...original research experiences for thousands of undergraduates. The MCC develops and makes freely available...production.   CUREs are beginning to transform undergraduate education in the sciences by making a greater... State College in New Hampshire. He mentors undergraduates conducting NIH- and NSF-funded research on ...Bangera, G., Brownell, S. 2014. Course-based undergraduate research experiences can make scientific research...

...Addgene Podcast, we introduce you to the Journal of Emerging Investigators, an open-access journal that enables...Addgene. Listen to the interview with the Journal of Emerging Investigators Podcast breakdown Intro Chapter ... Springel, Olivia Ho-Shing, and the Journal of Emerging Investigators. Chapter 2 - Getting high school...

... lower the pipette tip into the liquid. Do not submerge the pipette itself into the liquid and avoid touching...contaminating the contents. Once the end of the tip is submerged, slowly release the plunger, allowing it to go...

... a water bath at 60 ℃ with sufficient water to submerge ~75% of the bottle containing your molten gel ...removing anything from the autoclave. Partially submerge your molten gel-mix in the 60 ℃ water bath. *Pro-Tip...

...dvpr and envelope plasmid pCMV-VSVG from Robert Weinberg’s lab. For more information, visit Addgene’s Mammalian... pCMV-dR8.2 dvpr and pCMV-VSVG from the Robert Weinberg lab. For more information, visit Addgene’s Mammalian...

...bare hand to open doors. For those with latex allergies, be aware that some gloves may contain latex. ...

...cells to completely die. After that, the cells may undergo additional seleciton while the population expands...

...Pro-Tip* Because the polyclonal cell pool is always undergoing selection, it’s best to generate monoclonal cell...

...cells is critical for optimal AAV yield. Do not overgrow your cells. Pass the cells twice a week during...

...Example dilutions and titrations Reference Wang Y, Bergelson S, Feschenko M. Determination of Lentiviral Infectious...

...-dependent dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer...recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer...

...Fibroblasts Dopaminergic Neurons Lentiviral Human Direct conversion of human fibroblasts to dopaminergic neurons...fibroblasts into cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fetal Lung Fibroblasts Cholinergic Neurons...Biotechnology. 2014 Dec;32(12):1223-30. Zhou Astrocytes Dopaminergic Neurons Lentiviral Human Efficient conversion...conversion of astrocytes to functional midbrain dopaminergic neurons using a single polycistronic vector. PLoS...10(4):465-72. Schöler Adult Skin Fibroblasts Cholinergic Neurons Lentiviral Human Small molecules enable...to efficiently convert human fibroblasts into cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fibroblasts...neurons. Unpublished. Ward Glial Progenitor Cells GABAergic Interneurons Lentiviral Human Direct Conversion...

...Targeted sensors for glutamatergic neurotransmission Targeted sensors for glutamatergic neurotransmission...fluorescent protein (cpFP) or Förster resonance energy transfer (FRET) efficiency of a pair of fluorescent...Cohen Calcium GCaMP6f expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer...Griesbeck Lab Twitch Plasmids Oliver Griesbeck Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca+ dynamics...Fluorescent sensor of ATP:ADP ratio (PercevalHR) Imaging energy status in live cells with a fluorescent biosensor...and metabolism (FLIPmal) Fluorescence Resonance Energy Transfer sensors for quantitative monitoring of... acetylcholine sensor for monitoring in vivo cholinergic activity (GACh3.0) (Constitutive or Cre-dependent...

...browse the Weinberg Lab's plasmid page and search for ‘pBabe’ Land , Morgenstern , Weinberg 1780 pBABE-neo...of immortalized cells; see Weinberg Lab page for resistance variants Weinberg 1773 pBABE-hygro-hTERT MoMLV...Contains gag and pol; Use with envelope plasmid Weinberg 12371 pCL-Eco Packaging Contains gag, pol, & env...Envelope Envelope protein; Use with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope...plasmids include Puro resistance ; Neo Resistance Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA...

...surface-exposed positively-charged grooves on Cas9. Cas9 undergoes a conformational change upon gRNA binding that... PAM often still permit target cleavage. Cas9 undergoes a second conformational change upon target binding..., Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS. Nat Microbiol . Feb;4(2):244-250. PMID: 30617347...L, Hirano S, Okazaki S, Noda T, Abudayyeh OO, Gootenberg JS, Mori H, Oura S, Holmes B, Tanaka M, Seki ..., Hegde M, Donovan KF, Strand C, Sullender ME, Vaimberg EW, Goodale A, Root DE, Piccioni F, Doench JG....Kleinstiver BP, Welch MM, Sousa AA, Harrington LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA. Nature . 550...DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, Wu WY, Scott DA, Severinov K, van der Oost...

...watermelon mutant clones for your course-based undergraduate research experience (CURE) projects....Community (MCC) Collection Blog post: Course-Based Undergraduate Research Experiences (CUREs) Background The ...window) develops protein-centric Course-based Undergraduate Research Experiences (CUREs) centered on a single...opens in a new window) Length of course-based undergraduate research experiences (CURE) impacts student ...Increasing access for biochemistry research in undergraduate education: The malate dehydrogenase CURE community...

...synthesis, and increases energy production through glycolysis to fuel these energy-intensive processes. mTORC1...signals, including amino acids, stress, oxygen, energy levels, and growth factors. mTORC1 integrates these...including an abundant supply of macromolecules and energy, as well as the presence of both pro-proliferative...machinery in cancer. Bhat M, Robichaud N, Hulea L, Sonenberg N, Pelletier J, Topisirovic I. Nat Rev Drug Discov...

...TARDBP CMV ALS Shawn Ferguson 133754 pLVX-Puro-TDP-43-A90V TARDBP CMV ALS Shawn Ferguson 133755 pLVX-Puro-...TARDBP CMV ALS Shawn Ferguson 133756 pLVX-Puro-TDP-43-A382T TARDBP CMV ALS Shawn Ferguson 133757 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133758 pLVX-Puro-TDP-43-K263E TARDBP CMV ALS Shawn Ferguson 133759 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133760 pLVX-Puro-TDP-43-P112H TARDBP CMV ALS Shawn Ferguson 133761 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133762 px459-TDP-43 sgRNA1 TARDBP U6 ALS Shawn Ferguson 133763 px459-TDP 43...diseases was curated from several sources (Chan Zuckerberg Initiative, MedGen, ClinVar) and is meant to ...eIF4GI (1-1599) EIF4G1 HA CMV Parkinson's Nahum Sonenberg 45875 pEGFP-parkin WT PRKN GFP CMV Parkinson's...