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Showing: 1 - 8 of 8 results

  1. Pipetting Protocol

    Type
    Protocol
    ... lower the pipette tip into the liquid. Do not submerge the pipette itself into the liquid and avoid touching...contaminating the contents. Once the end of the tip is submerged, slowly release the plunger, allowing it to go...

  2. Pouring LB Agar Plates

    Type
    Protocol
    ... a water bath at 60 ℃ with sufficient water to submerge ~75% of the bottle containing your molten gel ...removing anything from the autoclave. Partially submerge your molten gel-mix in the 60 ℃ water bath. *Pro-Tip...

  3. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...dvpr and envelope plasmid pCMV-VSVG from Robert Weinberg’s lab. For more information, visit Addgene’s Mammalian... pCMV-dR8.2 dvpr and pCMV-VSVG from the Robert Weinberg lab. For more information, visit Addgene’s Mammalian...

  7. AAV Production in HEK293 Cells

    Type
    Protocol
    ...cells is critical for optimal AAV yield. Do not overgrow your cells. Pass the cells twice a week during...

  8. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Example dilutions and titrations Reference Wang Y, Bergelson S, Feschenko M. Determination of Lentiviral Infectious...
Showing: 1 - 8 of 8 results