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Showing: 1 - 20 of 55 results

  1. Brain Initiative Collection

    Type
    Collection
    ...-dependent dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer...recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer...

  2. Plasmids for Stem Cell Research

    Type
    Collection
    ...Fibroblasts Dopaminergic Neurons Lentiviral Human Direct conversion of human fibroblasts to dopaminergic neurons...fibroblasts into cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fetal Lung Fibroblasts Cholinergic Neurons...Biotechnology. 2014 Dec;32(12):1223-30. Zhou Astrocytes Dopaminergic Neurons Lentiviral Human Efficient conversion...conversion of astrocytes to functional midbrain dopaminergic neurons using a single polycistronic vector. PLoS...10(4):465-72. Schöler Adult Skin Fibroblasts Cholinergic Neurons Lentiviral Human Small molecules enable...to efficiently convert human fibroblasts into cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fibroblasts...neurons. Unpublished. Ward Glial Progenitor Cells GABAergic Interneurons Lentiviral Human Direct Conversion...

  3. Retrovirus Plasmids

    Type
    Collection
    ...browse the Weinberg Lab's plasmid page and search for ‘pBabe’ Land , Morgenstern , Weinberg 1780 pBABE-neo...of immortalized cells; see Weinberg Lab page for resistance variants Weinberg 1773 pBABE-hygro-hTERT MoMLV...Contains gag and pol; Use with envelope plasmid Weinberg 12371 pCL-Eco Packaging Contains gag, pol, & env...Envelope Envelope protein; Use with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope...plasmids include Puro resistance ; Neo Resistance Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA...

  4. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Targeted sensors for glutamatergic neurotransmission Targeted sensors for glutamatergic neurotransmission...fluorescent protein (cpFP) or Förster resonance energy transfer (FRET) efficiency of a pair of fluorescent...Cohen Calcium GCaMP6f expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer...Griesbeck Lab Twitch Plasmids Oliver Griesbeck Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca+ dynamics...Fluorescent sensor of ATP:ADP ratio (PercevalHR) Imaging energy status in live cells with a fluorescent biosensor...and metabolism (FLIPmal) Fluorescence Resonance Energy Transfer sensors for quantitative monitoring of... acetylcholine sensor for monitoring in vivo cholinergic activity (GACh3.0) (Constitutive or Cre-dependent...

  5. CRISPR Guide

    Type
    Collection
    ...surface-exposed positively-charged grooves on Cas9. Cas9 undergoes a conformational change upon gRNA binding that... PAM often still permit target cleavage. Cas9 undergoes a second conformational change upon target binding..., Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS. Nat Microbiol . Feb;4(2):244-250. PMID: 30617347...L, Hirano S, Okazaki S, Noda T, Abudayyeh OO, Gootenberg JS, Mori H, Oura S, Holmes B, Tanaka M, Seki ..., Hegde M, Donovan KF, Strand C, Sullender ME, Vaimberg EW, Goodale A, Root DE, Piccioni F, Doench JG....Kleinstiver BP, Welch MM, Sousa AA, Harrington LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA. Nature . 550...DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, Wu WY, Scott DA, Severinov K, van der Oost...

  6. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...watermelon mutant clones for your course-based undergraduate research experience (CURE) projects....Community (MCC) Collection Blog post: Course-Based Undergraduate Research Experiences (CUREs) Background The ...window) develops protein-centric Course-based Undergraduate Research Experiences (CUREs) centered on a single...opens in a new window) Length of course-based undergraduate research experiences (CURE) impacts student ...Increasing access for biochemistry research in undergraduate education: The malate dehydrogenase CURE community...

  7. mTOR Pathway

    Type
    Collection
    ...synthesis, and increases energy production through glycolysis to fuel these energy-intensive processes. mTORC1...signals, including amino acids, stress, oxygen, energy levels, and growth factors. mTORC1 integrates these...including an abundant supply of macromolecules and energy, as well as the presence of both pro-proliferative...machinery in cancer. Bhat M, Robichaud N, Hulea L, Sonenberg N, Pelletier J, Topisirovic I. Nat Rev Drug Discov...

  8. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...TARDBP CMV ALS Shawn Ferguson 133754 pLVX-Puro-TDP-43-A90V TARDBP CMV ALS Shawn Ferguson 133755 pLVX-Puro-...TARDBP CMV ALS Shawn Ferguson 133756 pLVX-Puro-TDP-43-A382T TARDBP CMV ALS Shawn Ferguson 133757 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133758 pLVX-Puro-TDP-43-K263E TARDBP CMV ALS Shawn Ferguson 133759 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133760 pLVX-Puro-TDP-43-P112H TARDBP CMV ALS Shawn Ferguson 133761 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133762 px459-TDP-43 sgRNA1 TARDBP U6 ALS Shawn Ferguson 133763 px459-TDP 43...diseases was curated from several sources (Chan Zuckerberg Initiative, MedGen, ClinVar) and is meant to ...eIF4GI (1-1599) EIF4G1 HA CMV Parkinson's Nahum Sonenberg 45875 pEGFP-parkin WT PRKN GFP CMV Parkinson's...

  9. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...Background Förster resonance energy transfer, or FRET , is a process by which energy is non-radiatively transferred...fluorophore to an acceptor. Since the transfer of energy does not occur by emission of a photon, the acceptor...pairs in FRET experiments. The efficiency of the energy transfer is measured using fluorescence microscopy...

  10. Lentivirus Plasmids

    Type
    Collection
    ...making Integrase deficient lentivirus Rawlings , Scharenberg 12251 pMDLg/pRRE 3rd Packaging Contains Gag and...N/A Envelope VSV-G-expressing envelope plasmid Weinberg 8455 pCMV-dR8.2 dvpr 2nd Packaging Contains Gag... with 2nd and 3rd generation transfer plasmids Weinberg 1733 pCI-VSVG N/A Envelope VSV-G recommended for...resistance. See plasmid 24150 for hygro resistance. Weinberg 10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven...

  11. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Kleinstiver BP, Welch MM, Sousa AA, Harrington LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA. 2017. Enhanced...PMID: 27984730 Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova...Science . 343(6166):80-4. PMID: 24336569 Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, ...DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, Wu WY, Scott DA, Severinov K, van der Oost...

  12. Zhang Lab CRISPR Page

    Type
    Collection
    ...activation plasmids and screening library CRISPR/Cas9 Synergistic Activation Mediator (SAM) is an engineered protein... editing specificity. Ran FA, Hsu PD, Lin CY, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue ...J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature . ...aureus Cas9. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova...

  13. Antibody Production

    Type
    Collection
    ...production, all antibody preps are titered and undergo stringent quality control by Addgene or a trusted...Buffer After affinity chromatography, antibodies undergo a buffer exchange using centrifugal columns. The...distributed. Quality Control Each lot of antibody undergoes testing, either in-house or through outside labs...

  14. Mammalian RNAi Tools

    Type
    Collection
    ...puro Retroviral vector for shRNA expression Bob Weinberg 10675 pMKO.1 puro GFP shRNA Negative control vector... arrayed viral high-content screen. Moffat J, Grueneberg DA, Yang X, Kim SY, Kloepfer AM, Hinkle G, Piqani...An DS, Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD. RNA. 2003. 9(4):493-501. PubMed...

  15. Neurodegeneration Research Collection

    Type
    Collection
    ... Next-generation GRAB sensors for monitoring dopaminergic activity in vivo. Coexpressing red GRABDA with...calcium indicator GCaMP6s allows tracking of dopaminergic signaling and neuronal activity in distinct ...resource was created with support from the Chan Zuckerberg Initiative (Link opens in a new window) ....

  16. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...3rd 10 Varies Berger Lab Human Paralog Knockout Library (pgPEN) 171172 Knockout Human Berger 3rd 8-16 33,170...

  17. Deisseroth INTRSECT Collection

    Type
    Collection
    ...delayed excitation in lateral dorsal striatal cholinergic interneurons. Elife. 7. pii: e39786. PMID:30295607...Brain-Wide Afferent Inputs of Parvalbumin-Expressing GABAergic Neurons in Barrel Cortex Reveals Local and Long-Range..., Fuzik J, Fürth D, Fenno LE, Ramakrishnan C, Silberberg G, Deisseroth K, Carlén M, Meletis K. 2019. A...

  18. Brain Armamentarium

    Type
    Collection
    ... strong & rapid SYFP2 expression in forebrain GABAergic interneurons. The enhancer core has 100% seq conservation...BGHpA AAV vector for Cre expression in forebrain GABAergic interneurons. For use in combination with Cre-...

  19. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...visit iGEM: Silver Standard (RFC 23) Back to Top Freiberg Standard (RFC 25) Prefix: GAATTC GCGGCCGC T TCTAGA...NgoMIV, AgeI, SpeI, PstI For more info, visit iGEM: Freiberg Standard (RFC 25) Back to Top...

  20. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...ancestral capsid Anc80L65 with endogenous AAP Vandenberghe 68837 pAnc80L65 Anc80L65 AAV packaging plasmid...expressing Rep2 and ancestral capsid Anc80L65 Vandenberghe 78504 pGG-B1 B1 AAV packaging plasmid, expressing...
Showing: 1 - 20 of 55 results