...viral service items we employ dozens of pieces of energy-intensive lab equipment on a daily basis. By the...allows a community like Watertown to bulk purchase energy from a higher percentage of renewable sources. ... drawing 100% of our electricity from renewable energy projects right here in New England- the majority...ventilation, and lab lighting/HVAC requires a significant energy expenditure here at Addgene. These are critical... or substantially reduced in an attempt to save energy. The transition to 100% renewable electricity will... will allow us to maintain these particularly energy-intensive systems in a more environmentally responsible...

...scientists I’ve met began doing research in their undergraduate years. We each have a unique “origin story,”... provide some perspective and advice to any undergraduate interested in getting a taste of the role of...Professor Sometimes teaches graduate and/or undergraduate classes Postdoctoral researcher (postdoc...choosing one as their official thesis lab Undergraduate student Roles and responsibilities can vary...would also highly recommend talking to other undergraduate researchers, as well as graduate students (your...’s something you want to dedicate your time and energy to. How can I make the most of my experience in...valuable for anyone in the lab though, including undergrads. I found a lot of career resources on Science...

... in their careers? A CURE for Biochemistry Undergraduate biochemistry courses are often associated with... what is now commonly termed a Course-based Undergraduate Research Experience (CURE). Having incorporated...Knutson et al., 2010a,b).      Fig. 1: Undergraduates in Provost’s CURE prepare to analyze their ...original research experiences for thousands of undergraduates. The MCC develops and makes freely available...production.   CUREs are beginning to transform undergraduate education in the sciences by making a greater... State College in New Hampshire. He mentors undergraduates conducting NIH- and NSF-funded research on ...Bangera, G., Brownell, S. 2014. Course-based undergraduate research experiences can make scientific research...

...Addgene Podcast, we introduce you to the Journal of Emerging Investigators, an open-access journal that enables...Addgene. Listen to the interview with the Journal of Emerging Investigators Podcast breakdown Intro Chapter ... Springel, Olivia Ho-Shing, and the Journal of Emerging Investigators. Chapter 2 - Getting high school...

...antibodies. Detergents  Another common family of permeabilization reagents are detergents, such as Triton...these detergents avoid some of the negative effects caused by organic solvents. However, detergents can ...for what reagents to use - organic solvents and detergents.  Organic Solvents  As mentioned above, organic...Triton X-100, Tween-20, or saponin. Each detergent has different chemical properties that will impact the... X-100 and Tween-20 are examples of non-ionic detergents that interact with lipids and proteins in membranes...protein. B) Permeabilizing with the selective detergent, saponin, keeps membrane-associated proteins in... epitopes. C) Permeabilizing with a non-ionic detergent, like Triton X-100, can lose membrane-associated...

...guest blogger, Leo Vo, a PhD candidate in the Sternberg Lab at Columbia University Medical Center. DNA...with the discovery of CRISPR-transposons by the Sternberg and Zhang labs (Peters et al., 2017; Klompe et...Elements by Guide RNA-Assisted Targeting) by the Sternberg group, and CAST (CRISPR-associated Transposase...the CRISPR-transposon systems developed by the Sternberg lab derives from Vibrio cholerae (V. cholerae ...CRISPR-transposons for bacterial genomes engineering In the Sternberg group’s initial report of VchINT, three separate...Vo! Leo Vo is currently a PhD candidate in the Sternberg Lab at Columbia University Medical Center. He ....     References: Halpin-Healy TS, Klompe SE, Sternberg SH, Fernández IS (2020) Structural basis of DNA...

... and what's it good for? Fluorescence resonance energy transfer (FRET) is a biophysical interaction between...fluorophore (the donor) is excited, it can transfer energy directly via resonance to the second fluorophore...fluorophore labeled and that these fluorophores can undergo FRET when they are near each other. How do you ...still develop a successful FRET-based assay since energy transfer only occurs when donor and acceptor are...acceptors >100 Å away from the donor doesn’t result in energy transfer and thus, your specific FRET signal is...donors never “see” FRET acceptors, and thus never undergo FRET. If this nonspecific labeling far exceeds ...experiments will define the maximum and minimum energy transfer values you can expect, thereby establishing...

...What is this FRET you speak of? Förster Resonance Energy Transfer (FRET) was originally described by Theodor... the alternative acronym Fluorescence Resonance Energy Transfer. Unlike the typical excitation and emission...fluorophore, FRET involves a non-radiative transfer of energy (i.e. no emitted photons) from the excited donor...fluorophore excitation by absorption of a photon 2. Energy transfer from the excited donor directly to the...spectrum of the acceptor fluorophore. Otherwise the energy transferred from the donor will not be able to ...of each other and be oriented appropriately for energy transfer via dipole-dipole interaction. The efficiency...M., et al. "Quantification of Förster resonance energy transfer by monitoring sensitized emission in living...

...have acquired divergent functions due to mutations accumulated over time? Undergraduates can help to answer...providing authentic research experiences to undergraduate students (1, 2), but educators face significant...Time project provides a framework for these undergraduate research questions. The idea is straightforward...cerevisiae. The two yeasts are thought to have diverged from a common ancestor ~350 million years ago.... have been largely conserved since the species diverged. Tips on using the Pathways Over Time plasmids... in strategies to incorporate research into undergraduate courses and in communicating science to the ...Advancement of Science. (2011). Vision and Change in Undergraduate Biology Education: A Call to Action, Washington...

...Reporter UnLOCKING (SHERLOCK and SHERLOCKv2) (Gootenberg et al., 2017 and 2018). While the Doudna lab ...not been approved for diagnostics yet, but is undergoing validation studies.     Jennifer Tsang contributed...updating this article. References Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DBT...439 . https://doi.org/10.1126/science.aar6245 Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy...442 . https://doi.org/10.1126/science.aam9321 Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins...10.1126/science.aaq0179 Myhrvold C, Freije CA, Gootenberg JS, Abudayyeh OO, Metsky HC, Durbin AF, Kellner...Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann...

...other reagents in biology. Luk H. Vandenberghe, PhD Luk Vandenberghe’s first lab experience was in a virology... Khandan Baradaran, Frank Stegmeier, and Luk Vandenberghe. We’re excited to grow the team and bring on...worked on vaccines throughout that time. Now Vandenberghe is an Associate Professor at Mass Eye and Ear... to develop an AAV-based COVID-19 vaccine.  Vandenberghe’s work with AAVs led to many interactions with...ultimately make innovation and science slow,” Vandenberghe says. “The continuous creativity that Addgene...Karen Guerin for the journal Molecular Therapy. Vandenberge is also the Grousbeck Family Chair in Gene Therapy...

...Plasmids: History & Biology The Joshua Lederberg Papers Joshua Lederberg's Personal Perspective: Plasmid (1952...Pubmed PMID: 13003535. Lederberg, Joshua, Luigi L. Cavalli, and Esther M. Lederberg. “Sex Compatibility ...did plasmids get their name? In 1952, Joshua Lederberg set out to clarify the classification of these...the Fertility, or F-factor discovered by Ester Lederberg in 1952 was noted to integrate into the E. coli...PubMed Central PMCID: PMC3785787. References: Lederberg, Joshua. "Cell Genetics and Hereditary Symbiosis...

...Bioluminescence Resonance Energy Transfer CRET= Chemiluminescence Resonance Energy Transfer    Interesting...well-known FRET, aka Fluorescence (Förster) Resonance Energy Transfer, technique which allows the detection ..."a distance-dependent physical process by which energy is transferred non-radiatively from an excited ... may be higher than the signal generated by the energy transfer. On the contrary, if you choose a pair... development of new fluorescent pairs and the emergence of faster measurement systems, this technique .... Sekar RB, Periasamy A. Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell...

...summer internship information sessions to help undergraduate students with their applications. The society...society currently has 1,283 members, who comprise undergraduates, graduate students, postdocs, faculty members...The society encourages interactions between undergraduates and PhD students so that they can help support...society currently has 352 members, ranging from undergrad all the way up to professors. Oxford Biological...at Imperial College London for students in this emerging field. It has gained over 170 members in its first...or biological societies were you a part of in undergrad or grad school? How did these societies help (...

...blogger Iris Lindberg, Professor at the University of Maryland School of Medicine. In the Lindberg Lab we ...blogger Iris Lindberg, Professor at the University of Maryland School of Medicine. Iris Lindberg is a professor...PC12, Rin5F, Neuro2A and GH4C1, among others (see Lindberg papers in PubMed). While we found that nearly ...neural and endocrine cells. She can be emailed at ilindberg@som.umaryland.edu.   Additional Resources on the...

... any bacteriologist’s skill set. So when Dave Westenberg taught this concept in his microbiology lab course...wear a mask. (@GermJuggler) February 13, 2021 Westenberg, a Professor of Biological Sciences at Missouri...by using a thicker bacterial suspension,” says Westenberg. In addition to streaking for single colonies...them into different strains of E. coli,” says Westenberg. During the transformation experiment, he plans...efficiency.  Aside from the Streak Plate Challenge, Westenberg’s course includes Winogradsky columns and characterizing...

...fluorescent microscopy. Collimated (non-converging or diverging) excitation light exits the microscope ...its unrivaled contrast, fluorescence imaging has emerged as the dominant light microscopy contrast technique...GFP. Because red light has approximately half the energy of blue light, two photons of red light are needed...Although the excitation light is completely reflected, energy is propagated into the sample via an evanescent...the fluorophore’s interaction with an evanescent energy field. Lightsheet microscopes that project ultra-thin...

...after undergrad] was to learn and speak the language of science. I didn’t come out of [undergrad] feeling...solidified some concepts that are heard often during undergrad. Lauren Flynn is the Addgenie behind our...doctor. After taking a year break in between undergraduate school and studying for the MCAT, she decided...description.” Our resident bagel provider, source of energy, and conversation master knows that although her...

...science.   Acknowledgements Chan Zuckerberg Initiative Chan Zuckerberg Biohub Manuel Leonetti Ryan Leenay...Greg Dingle, a software engineer with the Chan Zuckerberg Initiative. We hereby announce the general availability... us your questions or comments to gdingle@chanzuckerberg.com. We're eager to hear your thoughts. Many...Greg Dingle is a software engineer with the Chan Zuckerberg Initiative, working on assignment at the Biohub...

...Cressey is a PhD Student in the labs of Dr. Elke Mühlberger and Dr. Rachel Fearns at Boston University School...Medicine, Department of Microbiology, National Emerging Infectious Disease Laboratories.  She is interested...The power of reverse genetics. Springer Berlin Heidelberg, 2004. 1-41. PubMed PMID:15298166. 3. Hoenen,...21699921. PubMed Central PMCID: PMC3586226. 4. Mühlberger, Elke, et al. "Comparison of the transcription... 9971816. PubMed Central PMCID: PMC104478. 5. Mühlberger, Elke. "Filovirus replication and transcription...