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Lenti-sgNf2#1/Cre

Plasmid

#173643

Purpose

Expresses a Nf2-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Nf2 (Nf2 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgSmad4#1/Cre

Plasmid

#173617

Purpose

Expresses a Smad4-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Smad4 (Smad4 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmThor-V5His6

Plasmid

#79408

Purpose

Expresses EGFP-tagged and C-terminal V5His6-tagged DmThor in S2 cells

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor (Thor Fly)

Use

Schneider 2 cell expression

Tags

EGFP and V5-His6

Promoter

Ac5 promoter

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pTBL681 CHYRON4 integration construct

Plasmid

#126449

Purpose

To integrate the CHYRON4 locus at AAVS1 in human cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

pU6/3xLacO-CHYRON4 hgRNA
pCMV-puro

Expression

Mammalian

Mutation

The SpCas9 sgRNA constant region is mutated to ma…

Promoter

CMV and human U6/3xLacO

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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PX459v2-ILK-gRNA 1-exon 1

Plasmid

#163320

Purpose

SpCas9 ILK gRNA 1 (G*CGGAGAACGACCTCAACCAG), targeting exon 1 within the N-terminal ankyrin repeat domain-1.

Depositor

Val Brunton

Article

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ILK gRNA 1_Exon1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
PX459v2-ILK-gRNA 2-exon 8

Plasmid

#163321

Purpose

SpCas9 ILK gRNA 2 (G*ACATTGTAGAGGGATCCATA), targeting exon 8 within the C-terminal protein kinase domain.

Depositor

Val Brunton

Article

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ILK gRNA 2_Exon8

Use

CRISPR

Expression

Mammalian

Promoter

U6F

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
C-His-GvpC-Calpain

Plasmid

#153295

Purpose

Expresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

Calpain-sensitive Gas vesicle protein C (his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

1) Contains an extra glycine after the methionine…

Promoter

T7

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-EF1α1.1-FLEX-rc [SomArchon-GFP]

Plasmid

#153532

Purpose

AAV-mediated expression of SomArchon-GFP under the EF1α1.1 promoter, in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon-GFP

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Promoter

EF1α1.1

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgSIK3-A

Plasmid

#138682

Purpose

Expresses a mouse SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgSIK3-C

Plasmid

#138683

Purpose

Expresses a mouse SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pTBL649 CHYRON2 integration construct

Plasmid

#126446

Purpose

To integrate the CHYRON2 locus at AAVS1 in human cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

pU6-CHYRON2 hgRNA
pCMV-puro

Expression

Mammalian

Mutation

The SpCas9 sgRNA constant region is mutated to ma…

Promoter

CMV and human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJC2297 - pHR-TRE3G-hSpCas9-NLS-FLAG-2A-Thy1.1

Plasmid

#250251

Purpose

Expression of Cas9 with Thy1.1 cell surface marker under a doxycycline responsive promoter. Requires additional expression of transactivator.

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

hSpCas9

Use

CRISPR and Lentiviral

Tags

NLS-FLAG-2A-Thy1.1

Promoter

TRE3G

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCBS-4804

Plasmid

#249320

Purpose

Expresses Non-targeting sgRNA and contains the Repair Template to replace the KanR* on the genome of E. coli MG1655

Depositor

Chase Beisel

Article

Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038

Insert

nontargeting sgRNA

Expression

Bacterial

Mutation

N/A

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pCBS-5740

Plasmid

#249327

Purpose

Expresses sgRNA targeting the fcy gene in S. cerevisiae

Depositor

Chase Beisel

Article

Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038

Insert

sgRNA targeting fcy

Expression

Yeast

Mutation

N/A

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pSBtet-FAP-TGN38-GFP

Plasmid

#242992

Purpose

Inducible FAP-TGN38-GFP expression in mammalian cells

Depositor

Itay Budin

Article

Moore et al Nat Chem Biol (2025). https://doi.org/10

Insert

dH6.2-TGN38-GFP (Tgoln2 Rat)

Use

Sleeping beauty

Tags

FAP and GFP

Expression

Mammalian

Promoter

Tight TRE

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSBtet-GFP-TGN38-FAP

Plasmid

#242993

Purpose

Inducible GFP-TGN38-FAP expression in mammalian cells

Depositor

Itay Budin

Article

Moore et al Nat Chem Biol (2025). https://doi.org/10

Insert

GFP-TGN38-dH6.2 (Tgoln2 Rat)

Use

Sleeping beauty

Tags

FAP and GFP

Expression

Mammalian

Promoter

Tight TRE

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSBtet-mCer3-TGN38-FAP

Plasmid

#242994

Purpose

Inducible mCer3-TGN38-FAP expression in mammalian cells

Depositor

Itay Budin

Article

Moore et al Nat Chem Biol (2025). https://doi.org/10

Insert

mCer3-TGN38-dH6.2 (Tgoln2 Rat)

Use

Sleeping beauty

Tags

FAP and mCer3

Expression

Mammalian

Promoter

Tight TRE

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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LentiX_(loxPconDIAL-YB_TATA)-mCherry-HRasG12V-BGH_pKG3906

Plasmid

#246345

Purpose

loxPcon DIAL Reporter Lentivirus expressing mCherry-HRasG12V in the presence of ZFa

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mCherry-GS-HRasG12V (HRAS Human)

Use

Lentiviral and Synthetic Biology

Mutation

G12V

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pEf1a-Dn29-dCas9-P2A-GFP

Plasmid

#247155

Purpose

Mammalian expression of a human codon optimized wildtype Dn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Dn29-dCas9-P2A-GFP

Tags

SV40 NLS

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pSG5-CAGEc-ABL1 (Npu)

Plasmid

#241420

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product BCR-ABL, Figure 3, Extended Figure 6-7 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

CAGEc-ABL1 (Npu) (ABL1 Human, Synthetic)

Tags

HA

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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