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pBITE-EGFRvIII-F269-OKT3

Plasmid

#190681

Purpose

Encodes an EGFRvIII-CD3 BITE protein (F269 clone)

Depositor

Scott McComb

Publication

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Anti-EGFRvIII-scFv-F269-modularized-OKT3-HisTag

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EFS

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-off TauRD (P301L/V337M)

Plasmid

#188572

Purpose

Dox-repressible expression of the Tau repeat domain

Depositor

Franz-Ulrich Hartl

Publication

Saha et al Nat Commun. 2023 Feb 2;14(1):560. doi: 1

Insert

Tau repeat domain (MAPT Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

P301L and V337M

Promoter

pTight TRE

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-PASS

Plasmid

#193970

Purpose

Monitoring the production of phosphatidic acid with GFP-PASS (phosphatidic acid biosensor with superior sensitivity)

Depositor

Guangwei Du

Publication

Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

Insert

PA biosenor

Expression

Mammalian

Promoter

CMV

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hSyn-mCherry-sesRNA-smFlag-tTA2-W3SL

Plasmid

#192066

Purpose

Expression of mCherry, sesRNA in mammalian cells, with smFlag and tTA2 as efRNA.

Depositor

Josh Huang

Publication

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for Mouse Ctip2

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-ExRai-AMPKAR

Plasmid

#192449

Purpose

ExRai-AMPKAR targeted to the lysosomal membrane.

Depositor

Jin Zhang

Publication

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

Lyso-ExRai-AMPKAR

Tags

Full-length LAMP1

Expression

Mammalian

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSEM246 - mosTI - unc-119 - MCS

Plasmid

#182343

Purpose

Empty cloning vector for mosTI(unc-119). MCS or Golden Gate (BsaI: tgcc - MCS - gagc)

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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SPLINTR BFP v1

Pooled library

#179776

Purpose

SPLINTR (Single-cell Profiling and LINeage TRacing) is a high diversity lentiviral barcode library for tracing clonal lineages using bulk and single-cell readouts.

Depositor

Mark Dawson

Publication

Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

Expression

Mammalian

Use

Lentiviral

Available since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAAV-P3-Alb-mScarlet

Plasmid

#183461

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-mScarlet fusion protein

Depositor

Hajime Hirase

Publication

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

mScarlet

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP/golgin-160

Plasmid

#180630

Purpose

Expression of WT golgin-160

Depositor

Carolyn Machamer

Publication

Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

Insert

golgin-160 (GOLGA3 Human)

Tags

EGFP

Expression

Mammalian

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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PiggyBac-TetO-Ascl1-neomycin

Plasmid

#176482

Purpose

TetO-Ascl1 in a PiggyBac vector with neomycin resistance

Depositor

Marius Wernig

Publication

Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

Insert

Ascl1 (Ascl1 Mouse)

Expression

Mammalian

Promoter

TetO

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-FLEX-P301L Tau

Plasmid

#176267

Purpose

This AAV plasmid vector expresses human 2N4R microtubule-associated protein tau with P301L mutation.

Depositor

Tsuneya Ikezu

Publication

You et al Neuroscience. 2019 Dec 1;422:65-74. doi:

Has service

AAV1

Insert

Flex-P301L tau (MAPT )

Use

AAV

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_PRMT1-Halo

Plasmid

#175334

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Publication

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

PRMT1 (PRMT1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAB2076 pAAV REPAIR.t1

Plasmid

#176323

Purpose

pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)

Depositor

Feng Zhang

Publication

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Inserts

human codon optimized Cas13bt1 (catalytically inactivated)
huADAR2dd(E488Q) (ADARB1 Human)
Cas13bt1 crRNA + BpiI cloning site

Use

AAV and CRISPR

Tags

3xHA and HIV NES

Expression

Mammalian

Mutation

E488Q, only the deaminase domain (aa 276-702 are …

Promoter

EFS (short EF1alpha) and hU6

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAB1620 hU6-BpiI-Cas13bt3-DR

Plasmid

#176315

Purpose

hU6-BpiI-Cas13bt3-DR (crRNA expression)

Depositor

Feng Zhang

Publication

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

Cas13bt3 crRNA direct repeat

Use

CRISPR

Expression

Mammalian

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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PGKp-GFP-TAZ

Plasmid

#174176

Purpose

Lentiviral vector to constitutively express TAZ/WWTR1 under control of the human PGK promoter.

Depositor

Rod Bremner

Publication

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

TAZ (WWTR1 Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

PGK

Available since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pET21-pelB-LaG-16

Plasmid

#172746

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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HC-M

Plasmid

#173481

Purpose

An rrnB P1-based GFP ATP reporter in E. coli

Depositor

Rahul Sarpeshkar

Publication

Deng et al BMC Biol. 2021 May 17;19(1):101. doi: 10

Insert

GFP-mut2

Use

Synthetic Biology

Tags

ssrA degradation tag

Expression

Bacterial

Promoter

rrnB P1

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Csy4-PE2

Plasmid

#172667

Purpose

Enhanced prime editing system in mammalian cells. This plasmid is used by PE3 and PE3b

Depositor

Xiaolong Wang

Publication

Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

Insert

Csy4-PE2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-1x

Plasmid

#172281

Purpose

Expressing EGFP mRNA fused with 1 tandem repeat of a 50-base sequence

Depositor

Antony K Chen

Publication

Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

Insert

EGFP-N1-1x

Expression

Mammalian

Promoter

CMV

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pYPQ132-tRNA2.0

Plasmid

#158394

Purpose

Golden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Publication

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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