We narrowed to 59,588 results for: ran

1. pcDNA-Flag-EcoCas5

   Plasmid

   #106273

   Purpose

   Encodes human codon optimized E.coli CRISPR Type I-E CasD with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   EcoCasD
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-EF1a-V5-Arl6ip6

   Plasmid

   #120235

   Purpose

   Expresses V5-tagged Arl6ip6 in lentiviral vector

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Insert

   Arl6ip6 (Arl6ip6 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Promoter

   EF1a

   Available Since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)mGAT1YFP*

   Plasmid

   #41665

   Depositor

   Henry Lester

   Article

   Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

   Insert

   Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   “Monomerizing” YFP A206K mutation; C-terminal hyd…

   Promoter

   CMV

   Available Since

   Jan. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28b Me31B-3Myc

   Plasmid

   #113001

   Purpose

   For protein expression and purification of Myc-tagged full-length Drosophila me31B

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   full length me31B (me31B Fly)
   

   Tags

   Myc

   Expression

   Bacterial

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. FR_HNF1B_P328L329del

   Plasmid

   #31102

   Depositor

   Gerhart Ryffel

   Article

   Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

   Insert

   HNF1B (HNF1B Human)
   

   Use

   Flp/frt

   Tags

   myc

   Expression

   Mammalian

   Mutation

   mutation P328L329delCCTCT

   Available Since

   Oct. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. Foxf1 pGEX4T1

   Plasmid

   #122213

   Purpose

   Expression of Foxf1 as a GST fusion protein in bacteria

   Depositor

   Paul Herring

   Article

   Hoggatt et al J Biol Chem. 2013 Oct 4;288(40):28477-87

   Insert

   Foxf1 (Foxf1 Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNUT N6His K206E hTFNG

   Plasmid

   #70130

   Purpose

   Expresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe

   Depositor

   Ross MacGillivray, Anne Mason

   Article

   He et al Biochemistry. 1999 Jul 27;38(30):9704-11

   Insert

   mutated human serum transferrin (TF Human)
   

   Tags

   N-terminal signal peptide, 4 aa link, 6 His, Fact…

   Expression

   Mammalian

   Mutation

   changed Lys206 to a glutamic acid, Asn413Asp, Asn…

   Promoter

   SV40

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAH-MIBP16

   Plasmid

   #51870

   Purpose

   Expresses C-terminal HA-tagged full length rat MIBP1 (cloned into pCAGGS)

   Depositor

   Kenshi Hayashi, Tomoko Tahira

   Article

   Iwashita et al J Biol Chem. 2012 Mar 23;287(13):9887-90

   Insert

   MIBP1 (Hivep2 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pMuLE ENTR SV40-hCas9 L5-L2

   Plasmid

   #62134

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   human codon optimized Cas9
   

   Use

   CRISPR; Mule gateway entry vector

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBEST-p70b-UTR1-deGFP-6xHis-T500

    Plasmid

    #92222

    Purpose

    Expression of deGFP using the p70b promoter (weak version of the p70a promoter)

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    deGFP
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    p70b (p70a promoter with one mutation)

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. mRuby2-Alpha-Actinin-19

    Plasmid

    #55890

    Purpose

    Localization: Alpha Actinin, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Alpha-Actinin (ACTN1 Human)
    

    Tags

    mRuby2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. WT MetNI in pBAD

    Plasmid

    #118254

    Purpose

    Wild Type Methionine ABC Transporter MetNI in pBAD Vector for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (metN Escherichia coli)
    MetI (metI Escherichia coli)
    

    Tags

    10x His Tag and Enterokinase Cleavage Site

    Expression

    Bacterial

    Promoter

    Arabinose Promoter and None

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMuLE ENTR CMV-hCas9 R4-R3

    Plasmid

    #62132

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing CMV promoter and human codon optimized Cas9 module Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    human codon optimized Cas9
    

    Use

    CRISPR; Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO_TAP_FAM86A

    Plasmid

    #85116

    Purpose

    mammalian expression of FAM86A

    Depositor

    Pal Falnes

    Article

    Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

    Insert

    EEF2KMT (FAM86A Human)
    

    Tags

    CBP-SBP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZF-AAA

    Plasmid

    #127465

    Purpose

    coding sequence for wild type zinc finger ZF-AAA (ZiF268)

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-AAA (EGR1 Human)
    

    Use

    Cell-free transcription–translation

    Promoter

    T7

    Available Since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBT2999

    Plasmid

    #122535

    Purpose

    Expression of a C. elegan codon optimized fluorescent protein (CemOrange2) fused to a lysosome localized gene (lmp-1) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_lmp-1::CemOrange2 (lmp-1 Synthetic, Nematode)
    

    Expression

    Bacterial

    Promoter

    Pnhx-2

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3_altPKA-Cα-mPAGFP

    Plasmid

    #114133

    Purpose

    Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    Testis only splice variant of mouse PKA catalytic subunit α that has no myristoylation site, C-terminally tagged by mPAGFP (Prkaca Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-CMV-cGASΔN C396/7A-HA

    Plasmid

    #130918

    Purpose

    Expresses human cGASΔN (aa160-522)-HA with C396/397A point mutation; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    cGASΔN C396/7A
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    mutated residues 396 and 397 to alanines

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBT3047

    Plasmid

    #122547

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a N-terminal (sv-40) and C-terminal (egl-13) nuclear localization signal under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_NLS(sv-40)::CemOrange2::NLS(egl-13)
    

    Tags

    MAPKKKRKVV and MSRRRKANPTKLSENAKKLAKEVEN

    Expression

    Bacterial

    Promoter

    Pnhx-2

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMAL c2x Tral FL

    Plasmid

    #113012

    Purpose

    For bacterial expression of MBP fusion of full-length Drosophila Tral WT

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length tral (tral Fly)
    

    Expression

    Bacterial

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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