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pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8-3

Plasmid

#216825

Purpose

Expresses acyclovir-controllable IRE variant #A8-3 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-IRE#A8-3

Expression

Mammalian

Promoter

U6+27

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8-4

Plasmid

#216826

Purpose

Expresses acyclovir-controllable IRE variant #A8-4 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-IRE#A8-4

Expression

Mammalian

Promoter

U6+27

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyA-GFP

Plasmid

#220189

Purpose

sgRNA expression vector - pEM7 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pEM7 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pEM7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyD-GFP

Plasmid

#220192

Purpose

sgRNA expression vector - pBG28 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG28 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG28

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRS-GFP

Plasmid

#220197

Purpose

mini-Tn7 GFP expression vector

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

monomeric superfolder green fluorescent protein

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

promoterless

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyA-GFP

Plasmid

#220189

Purpose

sgRNA expression vector - pEM7 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pEM7 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pEM7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpyD-GFP

Plasmid

#220192

Purpose

sgRNA expression vector - pBG28 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG28 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG28

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRS-GFP

Plasmid

#220197

Purpose

mini-Tn7 GFP expression vector

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

monomeric superfolder green fluorescent protein

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

promoterless

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
gRNA-hIRF-1 #12/pSIR-hCD2

Plasmid

#135392

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter

Depositor

Hodaka Fujii

Article

Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

Insert

gRNA_hIRF1 promoter #12

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA-hIRF-1 #12/pSIR-hCD2

Plasmid

#135392

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter

Depositor

Hodaka Fujii

Article

Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

Insert

gRNA_hIRF1 promoter #12

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRV∆L-5tTA

Plasmid

#182966

Purpose

The 3rd generation of rabies vector expressing a tetracycline-controlled transactivator (tTA)

Depositor

Ian Wickersham

Article

Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

tTA

Use

Rabies

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pRV∆L-5tTA

Plasmid

#182966

Purpose

The 3rd generation of rabies vector expressing a tetracycline-controlled transactivator (tTA)

Depositor

Ian Wickersham

Article

Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

tTA

Use

Rabies

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-9-SV40 polyA

Plasmid

#190873

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode9

Use

AAV

Mutation

WT

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-9-SV40 polyA

Plasmid

#190873

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode9

Use

AAV

Mutation

WT

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.F03R

Plasmid

#198664

Purpose

Easy-MISE toolkit pEM-plasmid containing mCherry coding sequence with HI protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

mCherry

Use

Synthetic Biology

Expression

Yeast

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.F03R

Plasmid

#198664

Purpose

Easy-MISE toolkit pEM-plasmid containing mCherry coding sequence with HI protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

mCherry

Use

Synthetic Biology

Expression

Yeast

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.F03L

Plasmid

#198663

Purpose

Easy-MISE toolkit pEM-plasmid containing mCherry coding sequence with CD protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

mCherry

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.F03L

Plasmid

#198663

Purpose

Easy-MISE toolkit pEM-plasmid containing mCherry coding sequence with CD protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

mCherry

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.F02R

Plasmid

#198662

Purpose

Easy-MISE toolkit pEM-plasmid containing GFP coding sequence preceded by a linker sequence with HI protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

linker+GFP

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.F02R

Plasmid

#198662

Purpose

Easy-MISE toolkit pEM-plasmid containing GFP coding sequence preceded by a linker sequence with HI protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

linker+GFP

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge