Addgene

We narrowed to 3,942 results for: eed

Showing: 1761 - 1800 of 3942 results

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pRetroSuper shAngptl4 hygro

Plasmid

#19154

Depositor

Joan Massague

Article

Padua et al Cell. 2008 Apr 4. 133(1):66-77.

Insert

small hairpin RNA against angiopoietin-like 4 (ANGPTL4 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pAG247

Plasmid

#33200

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

LRIG1 (LRIG1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

SV40 early enhancer/promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pSB117 - pL2_pSB90_fLUC-I_V2

Plasmid

#123192

Purpose

binary plant vector for transient TYLCV V2 and fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::V2::tNOS 2x35S::fLUC-I::tNOS

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET14b His Rab8a Q67L

Plasmid

#186014

Purpose

His Rab8a Q67L

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

His Rab8a Q67L (RAB8A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pSB115 - pL2_pSB90_fLUC-I_tGFP-I

Plasmid

#123190

Purpose

binary plant vector for transient tGFP (with intron) and fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::tGFP-I::tNOS 2x35S::fLUC-I::tNOS

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Tbx5

Plasmid

#72688

Purpose

Doxycyline regulated expression of Tbx5 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Tbx 5 (Tbx5 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV-Doxycycline

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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200 bp Axin2 mutant

Plasmid

#25704

Depositor

Eric Fearon

Article

Leung et al J Biol Chem. 2002 Jun 14. 277(24):21657-

Insert

Axin 2 promoter (AXIN2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

TCF DNA binding site at bp -108 to -102 mutated …

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS

Plasmid

#123188

Purpose

binary plant vector for transient fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::fLUC-I::tNOS and VirGN54D in the vector backbone

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRFAM7ADelta2bp-mGFP

Plasmid

#62636

Purpose

mammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with mEGFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Use

Tags

mEGFP inserted in M3-M4 loop

Expression

Mammalian

Mutation

there is a 2-bp deletion in exon 6 of CHRFAM7A ca…

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CHRFAM7ADelta2bp-mCherry

Plasmid

#62638

Purpose

mammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with mCherry fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Use

Tags

Expression

Mammalian

Mutation

there is a 2-bp deletion in exon 6 of CHRFAM7A an…

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only
pAG252

Plasmid

#33206

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

MAP4K4 3'UTR (MAP4K4 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

SV40 early enhancer/promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pAP1791-1

Plasmid

#105246

Purpose

human eGFP with homology arms for RAB11A (recoded )

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

eGFP with homology arms for RAB11A (recoded *) (RAB11A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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OMM-short-CFAST10

Plasmid

#233597

Purpose

Expression of CFAST10 on the OMM membrane after a short linker

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

CFAST10

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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PM-RspA-CFAST

Plasmid

#233601

Purpose

Expression of RspA-CFAST on the PM facing the cytosol

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

RspA-CFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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ER-RspA-CFAST

Plasmid

#233607

Purpose

Expression of RspA-CFAST on the ER membrane

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

RspA-CFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2D-A

Plasmid

#221713

Purpose

Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-A (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

D683A in active site

Promoter

simian CMV IE94

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pX552-EF1a-DIO Chronos-eGFP(with gRNA scaffold)

Plasmid

#199582

Purpose

Expresses Chronos-eGFP in a Cre-dependent manner

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Chronos eGFP

Use

AAV, CRISPR, and Cre/Lox

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1a intron A

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX552-DIO-NLS-mRuby3(with gRNA scaffold)

Plasmid

#199583

Purpose

Expresses NLS-mRuby3 in a Cre-dependent manner

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

NLS-mRuby3

Use

AAV, CRISPR, and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

U6 and EF1a

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG3-drAgo2DY-EF

Plasmid

#221733

Purpose

Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2DY-EF (ago2 Zebrafish)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

D651E and Y680F in active site

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2F-Y

Plasmid

#221722

Purpose

Expression of human Ago2 with F666Y mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2F-Y (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

F666Y in active site

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-FLAG3-hsAgo2E-D

Plasmid

#221721

Purpose

Expression of human Ago2 with E637D mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2E-D (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

E637D in active site

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-FLAG3-hsAgo2D-A

Plasmid

#221720

Purpose

Expression of human Ago2 with D669A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2D-A (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

D669A in active site

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-FLAG3-hsAgo2

Plasmid

#221719

Purpose

Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2 (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-FLAG3-drAgo2DY-EF

Plasmid

#221716

Purpose

Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2DY-EF (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

D651E and Y680F in active site

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-FLAG3-drAgo2Y-F

Plasmid

#221715

Purpose

Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2Y-F (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

Y680F in active site

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-FLAG3-drAgo2D-E

Plasmid

#221714

Purpose

Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-E (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

D651E in active site

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX552 EF1a DIO Chronos GFP_Vgat gRNA

Plasmid

#215277

Purpose

Expresses Vgat gRNA in a Cre dependent Chronos GFP vector

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Vgat gRNA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX552 EF1a DIO Chronos GFP_VgatTTT gRNA

Plasmid

#215278

Purpose

Expresses Vgat Control gRNA in a Cre dependent Chronos GFP vector

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Vgat control gRNA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX552-hSyn-DIO-GCaMP8f_Grin1 gRNA

Plasmid

#215281

Purpose

Expresses Grin 1 gRNA in a Cre dependent GCaMP8f vector

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Grin1 gRNA

Use

AAV

Tags

Expression

Mammalian

Mutation

See Depositor Comments Below

Promoter

U6

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
H2B-GFP-INCENP∆cen

Plasmid

#184044

Purpose

Expresses H2B-GFP fused to INCENP∆cen

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

INCENP (INCENP Human)

Use

Tags

GFP and H2B

Expression

Mammalian

Mutation

Truncated to 1-862

Promoter

CMV

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-mKO2-Parkin

Plasmid

#213544

Purpose

Transient mammalian expresssion of Parkin, N-terminally tagged with mKO2, M1 of Parkin intact

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Tags

mKO2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Parkin(E16A)

Plasmid

#213546

Purpose

Transient mammalian expresssion of E16A Parkin

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Tags

Expression

Mammalian

Mutation

E16A mutation in Parkin

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEMHE-MmCENP-O-GFP

Plasmid

#205178

Purpose

Expresses mouse CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcription

Depositor

Michael Lampson

Article

Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208

Insert

CENP-O (Cenpo Mouse)

Use

In vitro transcription

Tags

5 glycines linker followed by GFP

Expression

Mammalian

Mutation

Wild type mouse CENP-O

Promoter

T7

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
L_NLS-mutNLS V5-Twist

Plasmid

#201381

Purpose

Expresses Twist mutant for nucleocytoplasmic transport studies

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Twist (Twist1 Mouse)

Use

Tags

(NLS)SV40A4-V5

Expression

Mammalian

Mutation

K38R K73R

Promoter

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
M_NLS-mutNLS V5-Twist

Plasmid

#201379

Purpose

Expresses Twist mutant for nucleocytoplasmic transport studies

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Twist (Twist1 Mouse)

Use

Tags

(NLS)SV40A5-V5

Expression

Mammalian

Mutation

K38R K73R

Promoter

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mutNLS V5-Twist

Plasmid

#201380

Purpose

Expresses Twist mutant for nucleocytoplasmic transport studies

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Twist (Twist1 Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

K38R K73R

Promoter

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
H_NLS-mutNLS V5-Twist

Plasmid

#201378

Purpose

Expresses Twist mutant for nucleocytoplasmic transport studies

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Twist (Twist1 Mouse)

Use

Tags

(NLS)SV40-V5

Expression

Mammalian

Mutation

K38R K73R

Promoter

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
UL_NLS-mutNLS V5-Twist

Plasmid

#201382

Purpose

Expresses Twist mutant for nucleocytoplasmic transport studies

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Twist (Twist1 Mouse)

Use

Tags

(NLS)SV40A3-V5

Expression

Mammalian

Mutation

K38R K73R

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mut GBP2 V5-Twist

Plasmid

#201377

Purpose

Expresses Twist mutant for nucleocytoplasmic transport studies

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Twist (Twist1 Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

Y107E

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPBbleo-miRFP703-eDHFR(69K6)-Tiam1(DH-PH)

Plasmid

#186703

Purpose

Mammalian expression of Tiam1(DH-PH) fused to miRFP703-eDHFR(69K6)

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

miRFP703-eDHFR(69K6)-Tiam1(DH-PH)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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