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pTricHis-ILY-prepore-charge+

Plasmid

#160522

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-prepore-charge+

Tags

His-Tag

Expression

Bacterial

Mutation

I104C, G244C, Y336K, L340K

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-prepore-N342C

Plasmid

#160521

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-prepore-N342C

Tags

His-Tag

Expression

Bacterial

Mutation

I104C, G244C, N342C

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-prepore-L340C

Plasmid

#160520

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-prepore-L340C

Tags

His-Tag

Expression

Bacterial

Mutation

I104C, G244C, L340C

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTV-Trim28-tdiRFP670-PEST

Plasmid

#122367

Purpose

Targeting vector for mouse Trim28 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Trim28

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Trim27-tdiRFP670-PEST

Plasmid

#122356

Purpose

Targeting vector for mouse Trim27 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Trim27

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Trim28-R

Plasmid

#122339

Purpose

Expresses sgRNA targeting mouse Trim28 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Trim28

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Trim28-L

Plasmid

#122340

Purpose

Expresses sgRNA targeting mouse Trim28 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Trim28

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Trim27-L

Plasmid

#122317

Purpose

Expresses sgRNA targeting mouse Trim27 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Trim27

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Trim27-R

Plasmid

#122318

Purpose

Expresses sgRNA targeting mouse Trim27 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Trim27

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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p-AAV2-hSyn-MerMAID1-Citrine

Plasmid

#126520

Purpose

Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

Depositor

Peter Hegemann

Article

Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

Insert

MerMAID1

Use

AAV

Tags

Citrine

Promoter

human synapsin

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pESC/mCitrine/erg3-mCherry

Plasmid

#117993

Purpose

Co-expression of mCitrine under the control of the Gal1 promoter and erg3 fused to mCherry (an ER-targeting marker protein) under the control of the Gal10 promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

erg3-mCherry

Use

Plant/bacteria binary vector

Expression

Plant

Promoter

Gal10

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW Citrine-T2A-mTurquoise2

Plasmid

#118424

Purpose

Produces equimolar expression of Citrine and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

Citrine-T2A-mTq2

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pTriEX Tom20 mVenus BoPD

Plasmid

#114466

Purpose

Mammalian expression of mitochondrially targeted, mVenus labelled BoPD

Depositor

Andrew Woolley

Article

Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

Insert

BoPD

Tags

HIS, TEV and FLAG and TOM20 and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-dSestrin (CG11299-PD)

Plasmid

#73675

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn (variant D) (Sesn Fly)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pBS Matrilin-4 (clone 1_35)

Plasmid

#19192

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Matrilin 4 isoform 1 precursor (MATN4 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBS Matrilin-4 (clone 2_49)

Plasmid

#19218

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Matrilin 4 isoform 1 precursor (MATN4 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
KTD101(DE3)

Bacterial Strain

#138651

Purpose

KTD101(DE3) is a trigger factor deficient strain, which may increase protein secretion from Escherichia coli, and is compatible with expression from the T7 promoter

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Bacterial Resistance

None

Species

Escherichia coli

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only
pLV-CDH5-mCitrine-IRES-puro

Plasmid

#85143

Purpose

lentiviral expression of human VE-cadherin, C-terminally tagged with mCitrine

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

CDH5-mCitrine (VE-cadherin, C-terminally tagged with mCitrine (CDH5 Human)

Use

Lentiviral

Tags

mCitrine

Promoter

CAG

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCA27-pHR-MiniCMV-TriTag (mTagBFP)

Plasmid

#199458

Purpose

Expression of TriTag (mTagBFP) in mammalian cells

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

TriTag

Use

Lentiviral

Expression

Mammalian

Promoter

MiniCMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-tagRFP-FLAG-cGAS

Plasmid

#86676

Purpose

Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

tagRFP-FLAG-cGAS

Use

Lentiviral

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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