We narrowed to 5,835 results for: pCas

1. pCAD100_hIRG1_4-461_pvp008

   Plasmid

   #124875

   Purpose

   Expresses hCAD Arg273His mutant in E.coli

   Depositor

   Konrad Buessow

   Article

   Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

   Insert

   cis-aconitate decarboxylase (ACOD1 Human)
   

   Tags

   StrepTag and TEV site

   Expression

   Bacterial

   Mutation

   mutated Arg273 to His, deleted amino acids 1-3 an…

   Promoter

   T7

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-Blast_HP1beta_gRNA

   Plasmid

   #127908

   Purpose

   WT Cas9 Vector with Blasticidin Selection Marker targeting the 5' end of the human HP1beta gene

   Depositor

   Mark Groudine

   Insert

   gRNA for Human 5' HP1beta
   

   Use

   CRISPR

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAT.334

   Plasmid

   #119556

   Purpose

   Level T acceptor vector for chromosomal integration with lacZ compatible with blue/white screening. Modified pUC19 vector.

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. KA2963_eSpCas9-PGKHygdtkCh

   Plasmid

   #124205

   Purpose

   Expression of eSpCas9(1.1) and sgRNA

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9-J23113

   Plasmid

   #119701

   Purpose

   Expression of Cas9 with a weak promoter

   Depositor

   Chong Zhang

   Article

   Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCSD_SpCas9MT3-FKBP_SL

   Plasmid

   #107302

   Purpose

   Expresses R1335K mutant SpCas9 fused to FKBP in mammalian cells

   Depositor

   Scot Wolfe

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   FKBP

   Expression

   Mammalian

   Mutation

   R1335K and fused to FKBP

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS_m-AKAR5

   Plasmid

   #114139

   Purpose

   Mammalian expression of the AKAR5 sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   AKAR5 C-terminally tagged by KRas C tail
   

   Expression

   Mammalian

   Available Since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS_cyt-AKAR5

   Plasmid

   #114138

   Purpose

   Mammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   AKAR5 with C-terminal nuclear export signal (NES)
   

   Expression

   Mammalian

   Available Since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ptrc::zwf_fbp_opcA

   Plasmid

   #101846

   Purpose

   Replaces native promoter of zwf_fbp_opcA with Ptrc promoter

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   sp/st resistance casette followed by ptrc promoter
   

   Use

   Cloning vector

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBW751_pCAG-PV5

    Plasmid

    #87565

    Purpose

    Simple PV5

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBW752_pCAG-PV6

    Plasmid

    #87566

    Purpose

    Simple PV6

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBW755_pCAG-pDest-U5-UX

    Plasmid

    #89507

    Purpose

    pCAG destination vector (U5-UX)

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBW753_pCAG-PV7

    Plasmid

    #87567

    Purpose

    Simple PV7

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBW754_pCAG-PV8

    Plasmid

    #87568

    Purpose

    Simple PV8

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCASCADE-gapAP1

    Plasmid

    #87146

    Purpose

    gapAP1 targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    gapAP1 guide array
    

    Expression

    Bacterial

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCALNL_Ch5_155183064-155183141

    Plasmid

    #81211

    Purpose

    pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 5 (numbers in common name refer to pos. in hg38)

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    gix-Neo-gix deletion cassette upstream of EGFP
    

    Expression

    Mammalian

    Promoter

    pCBa

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-HDAC4*

    Plasmid

    #71402

    Purpose

    CAG promoter, mammalian expression

    Depositor

    Bo Chen

    Article

    Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

    Insert

    HDAC4* (HDAC4 Human)
    

    Expression

    Mammalian

    Mutation

    After aa126, insertion leads to reading frame shi…

    Promoter

    CAG

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pCASCADE-zwf

    Plasmid

    #65825

    Purpose

    zwf targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    zwf guide
    

    Expression

    Bacterial

    Promoter

    E . coli ugpB gene promoter, low phosphate induc…

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCASCADE-udhA

    Plasmid

    #65818

    Purpose

    udhA targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    udhA guide
    

    Expression

    Bacterial

    Promoter

    E . coli ugpB gene promoter, low phosphate induc…

    Available Since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCASCADE-proD

    Plasmid

    #65820

    Purpose

    Const. Promoter ProD targetting RNA E. coli Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    proD guide
    

    Promoter

    E . coli ugpB gene promoter, low phosphate induc…

    Available Since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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