We narrowed to 2,603 results for: pCas

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pCASP-SptP120-NS1-HilA

Plasmid

#153327

Purpose

Triggers secretion of SptP120 fused to the NS1 monobody from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-NS1 Monobody

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-eSpCas9n(1.1)-2A-EGFP-L2

Plasmid

#141052

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

eSpCas9(1.1)-2A-EGFP

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pET-FLAG-SpCas9-HF1

Plasmid

#126770

Purpose

Expression of increased fidelity SpCas9-HF1 in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

SpCas9-HF1

Use

CRISPR

Tags

3xFLAG, 6xHis, MBP, and NLS

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A

Promoter

T7

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSpCas9 (BB)-2A-GFP gamma-tubulin sg

Plasmid

#104437

Purpose

Knockdown the expression of gamma-tubulin in human cells

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

Insert

gamma-tubulin sgRNA

Tags

The gamma-tubulin sgRNA is coexpressed with 3xFLA…

Expression

Mammalian

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-Exon4-PAFAH1B1

Plasmid

#92433

Purpose

expresses Cas9 and gRNA targeting PAFAH1B1

Depositor

Ralf Brandes, Matthias Leisegang

Article

Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

Insert

PAFAH1B1 (PAFAH1B1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-HeFSpCas9-H840A

Plasmid

#80456

Purpose

high fidelity enhanced SpCas9 mammalian expression vector

Depositor

Ervin Welker

Insert

HeFSpCas9_H840A

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Promoter

CBh

Available Since

Aug. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-4 porcupine (genomic DNA)

Plasmid

#37376

Depositor

Norbert Perrimon

Article

Kadowaki et al Genes Dev. 1996 Dec 15;10(24):3116-28.

Insert

porcupine (por Fly)

Expression

Insect

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

Plasmid

#220493

Purpose

To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.

Depositor

Sébastien Britton

Article

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)

Use

CRISPR

Promoter

U6

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR

Plasmid

#113162

Purpose

A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable marker

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

SpCas9

Tags

FLAG

Expression

Mammalian

Mutation

D10A

Promoter

CAG

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4

Plasmid

#69227

Purpose

Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS4 ZFP

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8.20m-SpCas9-NRRH-P2A-EGFP (RMD47)

Plasmid

#197499

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRRH-BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

ABE8.20 mutations in TadA and nSpCas9-NRRH(D10A/I…

Promoter

CMV and T7

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8.20m-SpCas9-NRTH-P2A-EGFP (RMD63)

Plasmid

#197500

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRTH-BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

ABE8.20 mutations in TadA and nSpCas9-NRTH(D10A/I…

Promoter

CMV and T7

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMEH305 HA NLS SpCas9 3x high affinity MS2 loops

Plasmid

#168216

Purpose

Mammalian expression SV40 and nucleoplasmin NLS SpCas9 with 3 high affinity MS2 loops and fused to an N-terminal HA tag

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

SV40 NLS SpCas9

Use

Lentiviral

Tags

HA, SV40 NLS, and nucleoplasmin NLS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

Plasmid

#132560

Purpose

Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.

Depositor

Baisong Lu

Article

Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

Inserts

ABEmax
sgRNA, with Tetraloop replaced by com aptamer

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pspCas9-ABE-3'UTR-sgRNA-ST2-com vector

Plasmid

#136270

Purpose

Expresses ABE and ST2-com modified sgRNA scaffold

Depositor

Baisong Lu

Article

Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

Insert

ABE and sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCRI006-pYFAC-riboB-PgpdA-dSpCas9-VPR-TtrpC

Plasmid

#140199

Purpose

Episomal expression of dSpCas9-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Insert

dSpCas9-VPR

Use

CRISPR and Synthetic Biology; Expression in asper…

Tags

VPR (VP64-p65-Rta), NLS

Promoter

PgpdA

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCSD_2XNLS-SpCas9WT-NLS-3xHA-2XNLS-NmCas9WT-2XNLS

Plasmid

#107324

Purpose

Expresses SpCas9 fused to NmCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-NmCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 is fused to NmCas9

Promoter

CMV IE94

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI

Plasmid

#115210

Purpose

Expresses AspCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

AspCas13

Tags

SUMO

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9 (BB)-2A-GFP-RB1sg

Plasmid

#229857

Purpose

single guide RNA targeting human RB1 gene

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al FASEB J. 2025 Mar 15;39(5):e70431. doi:

Insert

RB1sgRNA (RB1 Human)

Tags

The gamma-tubulin sgRNA is coexpressed with 3xFLA…

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCas9-EcRT-Z3-Nat

Plasmid

#232094

Purpose

Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

Z3 promoter and Z3EV transcription factor

Use

CRISPR

Expression

Yeast

Promoter

Z3

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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