We narrowed to 18,340 results for: Mut

1. JDW 762 (pCS2-EGFP-hsKRAS4B-G12V)

   Plasmid

   #156407

   Purpose

   pCS2 CMV expression vector driving EGFP fused human KRAS4B-G12V

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4B-G12V (KRAS Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   mutant KRAS4B-G12V

   Promoter

   CMV

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBabe-Puro-BRAF-V600E

   Plasmid

   #15269

   Purpose

   Retroviral expression of BRAF V600E (the V600E mutation has been found in cancer patients, confers increased kinase activity, and is transforming)

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   BRAF (BRAF Human)
   

   Use

   Retroviral

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Valine 600 to Glutamate (mutation has been found …

   Promoter

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. dRED

   Plasmid

   #139836

   Purpose

   Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells

   Depositor

   Karim Mekhail

   Article

   Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

   Insert

   dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   RNase H1 protein contains D210N mutation. dCas9 …

   Promoter

   tet-inducible CMV

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

   Plasmid

   #103871

   Purpose

   dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Inserts

   PspCas13b
   huADAR2DD (ADARB1 Human)
   

   Use

   CRISPR

   Tags

   GS and HIV NES

   Expression

   Mammalian

   Mutation

   Changed histidne 133 to alanine and histidine 105…

   Promoter

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-KRAS

   Plasmid

   #116755

   Purpose

   Lentiviral expression of KRAS

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   KRAS (KRAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-MAPK1

   Plasmid

   #116760

   Purpose

   Lentiviral expression of MAPK1

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   MAPK1 (MAPK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLIK_hygro_3xflag_NRF2(RR)

   Plasmid

   #136535

   Purpose

   Lentiviral expression vector for an inducible 3xflag-tagged NRF2(a1584c_a1586t_a1589g)

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   NRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)
   

   Use

   Lentiviral; Destinatioin vector for gateway cloni…

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   This plasmid is developed by mutating 3 base pair…

   Promoter

   Available Since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18)

   Plasmid

   #207356

   Purpose

   Lentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   N1303K-CFTR + 13 C>G tevopreq1-prime editing guide RNA (epegRNA)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223_EGFR_p.ELREA_746del

   Plasmid

   #82911

   Purpose

   Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, Matthew Meyerson

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   EGFR (EGFR Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Deletion of residues 746-750 (ELREA)

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-Ef1a-mClover-W377A/W428A YTHDC1-T2A-BSD-siRNA-Resistant

    Plasmid

    #177130

    Purpose

    Generate lentivirus to introduce mClover tagged m6A binding null mutant W377A/W428A YTHDC1

    Depositor

    Wenbo Li

    Article

    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

    Insert

    YTHDC1 (YTHDC1 Human)
    

    Use

    Lentiviral

    Tags

    mClover3

    Expression

    Mutation

    W428A/W377A (m6A binding defects), and siRNA resi…

    Promoter

    Ef1a

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_BRAF_p.D594H

    Plasmid

    #82816

    Purpose

    Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, Matthew Meyerson

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    BRAF (BRAF Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    D594H

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. PGKp-GFP-YAP (S94A)

    Plasmid

    #174173

    Purpose

    Lentiviral vector to constitutively express TEAD-binding mutant YAP under control of the human PGK promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Insert

    YAP (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    3x FLAG tag

    Expression

    Mammalian

    Mutation

    Serine 94 mutated to Alanine

    Promoter

    PGK

    Available Since

    Aug. 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid

    #71685

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, S. pyogenes, Synthetic)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223_MAX_WT_V5

    Plasmid

    #82944

    Purpose

    Gateway Donor vector containing MAX, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, Matthew Meyerson

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    MAX (MAX Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pdCas9-DNMT3A-PuroR (ANV)

    Plasmid

    #71684

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Human, S. pyogenes, Synthetic)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223_CFLAR_WT_V5

    Plasmid

    #82936

    Purpose

    Gateway Donor vector containing CFLAR, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, Matthew Meyerson

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    CFLAR (CFLAR Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-KRAS-G12V

    Plasmid

    #116424

    Purpose

    Lentiviral expression of KRAS G12V

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    KRAS (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G12V

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-ICUE4

    Plasmid

    #181846

    Purpose

    Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    ICUE4 (RAPGEF3 Human)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-NES-ZapCV2

    Plasmid

    #222950

    Purpose

    Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

    Depositor

    Amy Palmer

    Article

    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

    Insert

    NES-ZapCV2 (NES Synthetic)
    

    Use

    Tags

    ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…

    Expression

    Mammalian

    Mutation

    ECFP gene is truncated 36 bp at 3' end. Venu…

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3-Flag-SPRTN-wt

    Plasmid

    #110214

    Purpose

    Expression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.

    Depositor

    Ivana Marinovic-Terzic, Kristijan Ramadan

    Article

    Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P296L (see depositor comments below)

    Promoter

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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