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pENTR-D-TOPO-kcnt1b

Plasmid

#164962

Purpose

Zebrafish kcnt1b gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnt1b (si:dkey-21e5.1 Zebrafish)

Use

Pcr cloning vector

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLNL1141

Plasmid

#160867

Purpose

PJ23110-RBS-PhCFAT-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

Coniferyl alcohol acyltransferase

Use

Synthetic Biology

Tags

mRuby2

Expression

Bacterial

Promoter

PJ23110

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLNL1127

Plasmid

#160859

Purpose

PJ23110-RBS-RhAAT1-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

Acetyl CoA geraniol/citronellol acetyltransferase

Use

Synthetic Biology

Tags

mRuby2

Expression

Bacterial

Promoter

PJ23110

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pJPS2951

Plasmid

#135585

Purpose

SNR6 has UU at position 105–106 deleted

Depositor

Jonathan Staley

Article

Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

Insert

snR6 (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6Δ2 has UU at position 105–106 deleted

Promoter

Native

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pJPS2954

Plasmid

#135588

Purpose

SNR6 has UAUUUCat position 102–107 deleted

Depositor

Jonathan Staley

Article

Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

Insert

snR6 (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6Δ6 hasUAUUUCat position 102–107 deleted

Promoter

Native

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pJPS2956

Plasmid

#135590

Purpose

SNR6 has GAUUUAUUUC at position 98–107 deleted

Depositor

Jonathan Staley

Article

Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

Insert

snR6 (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6Δ10 has GAUUUAUUUC at position 98–107 deleted

Promoter

Native

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pJPS2957

Plasmid

#135591

Purpose

SNR6 has GAGAUUUAUUUC at position 96–107 deleted

Depositor

Jonathan Staley

Article

Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

Insert

snR6 (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6Δ12 has GAGAUUUAUUUC at position 96–107 deleted

Promoter

Native

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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CRY2olig-BFP-YTHDF1(D401N)-C

Plasmid

#155354

Purpose

Lentivector to express human YTHDF1(264-559) with D401N mutation and CRY2olig-BFP at the N-terminal

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF1(264-559)

Use

Lentiviral

Expression

Mammalian

Mutation

D401N

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRS314_U6_A34G_A35G

Plasmid

#81232

Purpose

U6 telestem mutant (A34G/A35G) for in vivo studies

Depositor

Aaron Hoskins

Article

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with A34G/A35G mutation (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6 telestem mutant (A34G/A35G)

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRS314_U6_G96C_A97C

Plasmid

#81235

Purpose

U6 telestem mutant (G96C/A97C) for in vivo studies

Depositor

Aaron Hoskins

Article

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with G96C/A97C mutation (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6 telestem mutant (G96C/A97C)

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRS314_U6_U100C_U101C

Plasmid

#81236

Purpose

U6 telestem mutant (U100C/U101C) for in vivo studies

Depositor

Aaron Hoskins

Article

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with U100C_U101C mutation (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6 telestem mutant (G96C/A97C)

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pVW294

Plasmid

#139491

Purpose

Insertion of the pGRE2 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pGRE2 24xPP7sl

Expression

Yeast

Mutation

WT

Promoter

pGRE2

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pVW295

Plasmid

#139492

Purpose

Insertion of the pALD3 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pALD3 24xPP7sl

Expression

Yeast

Mutation

WT

Promoter

pALD3

Available Since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pSP266

Plasmid

#139488

Purpose

Insertion of the pCTT1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pCTT1 24xPP7sl

Expression

Yeast

Mutation

WT

Promoter

pCTT1

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSP264

Plasmid

#139487

Purpose

Insertion of the pSTL1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pSTL1 24xPP7sl

Expression

Yeast

Mutation

WT

Promoter

pSTL1

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSP566

Plasmid

#139500

Purpose

Gene tagging vector to add mCherry Tag and membrane anchoring motif

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

mCherry-CaaX

Expression

Yeast

Mutation

WT

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pVW200

Plasmid

#139489

Purpose

Insertion of the pGPD1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pGPD1 24xPP7sl

Expression

Yeast

Mutation

WT

Promoter

pGPD1

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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Opie2-NeoR[TraF]

Plasmid

#131617

Purpose

Expresses a functional NeoR only in females and dsRed in both genders.

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

Insert

NeoR[traF]

Expression

Insect

Promoter

Opie2

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pFETCh_MLLT10_iso1

Plasmid

#135729

Purpose

Donor vector for 3' FLAG tag of human MLLT10_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

MLLT10_iso1 Homology arms (MLLT10 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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PA-Bongwoori-R3

Plasmid

#137838

Purpose

A photoactivatable GEPI variant originated from ecliptic pHluorin

Depositor

Bradley Baker

Article

Lee et al Front Cell Neurosci. 2019 Oct 29;13:482.

Insert

Photoactivatable_Bongwoori-R3

Expression

Mammalian

Mutation

L64F, T65S, V163A, T203H, A227D

Promoter

CMV

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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