We narrowed to 15,992 results for: LEA

1. pCRII-Topo Grp

   Plasmid

   #45871

   Depositor

   Jeffrey Macklis

   Insert

   GRP in situ probe (Grp Mouse)
   

   Use

   In situ

   Mutation

   nt 66-504 on NM_175012

   Available Since

   June 11, 2013

   Availability

   Academic Institutions and Nonprofits only

2. RPRD2

   Plasmid

   #40740

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   RPRD2 (RPRD2 Human)
   

   Tags

   6xHis TEV

   Expression

   Bacterial

   Available Since

   Oct. 18, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

3. MPP8 (3R93)

   Plasmid

   #32050

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   MPP8 (MPHOSPH8 Human)
   

   Tags

   His (with TEV cleavage)

   Expression

   Bacterial

   Available Since

   Jan. 6, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pTH672-SUP35-Y351C

   Plasmid

   #29733

   Depositor

   Tobias von der Haar

   Insert

   SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   Y351C mutant gene of SUP35 including genomic sequ…

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-LaminB1-10

   Plasmid

   #55069

   Purpose

   Localization: Nuclear Envelope, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   LaminB1 (LMNB1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAT2

   Plasmid

   #112583

   Purpose

   Used for expression of protein in E. coli as non-cleavable minimal N-terminal 6His-tag fusions

   Depositor

   Johan Peränen, Marko Hyvönen

   Article

   Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

   Type

   Empty backbone

   Tags

   6His

   Expression

   Bacterial

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAS_MmaPylT_EF1_NES-MmaPylRS WT

   Plasmid

   #174901

   Purpose

   expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Tags

   FLAG and NES

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNIC-HIS6-MBP-TEV-GG

   Plasmid

   #215800

   Purpose

   Cloning of target gene with N terminal His6 MBP tag and TEV Cleavage

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pNIC-HIS6-NUSA-TEV-GG

   Plasmid

   #215798

   Purpose

   Cloning of target gene with N terminal His6 NUSA tag and TEV cleavage

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pNIC-HIS10-ZBASIC-TEV-GG

    Plasmid

    #215797

    Purpose

    Cloning of target gene with N-terminal His10 Zbasic tag and TEV cleavage

    Depositor

    Frank von Delft, ASAP Discovery

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pEGFP-C1 NFAT3

    Plasmid

    #10961

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

    Insert

    NFAT3 (NFATC4 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a MDHC_YEAST

    Plasmid

    #204232

    Purpose

    Bacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDHC_YEAST (MDH2 Budding Yeast)
    

    Tags

    6X His

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. PGK-AAVS1ZFNR

    Plasmid

    #60915

    Purpose

    encodes zinc finger nuclease specific to AAVS1 locus (right)

    Depositor

    Paul Gadue

    Article

    Sim et al Methods Mol Biol. 2015 Jan 29.

    Insert

    AAVS1-ZFNR
    

    Promoter

    PGK

    Available Since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.CAG.(mem).iGlucoSnFR2

    Plasmid

    #244077

    Purpose

    Plasma membrane targeted expression of green glucose sensor

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Insert

    iGlucoSnFR2
    

    Use

    AAV

    Tags

    IgG kappa leader

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato

    Plasmid

    #51083

    Purpose

    Cre dependent expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV Retrograde

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Promoter

    hEF1a

    Available Since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. PGK-AAVS1ZFNL

    Plasmid

    #60916

    Purpose

    encodes zinc finger nuclease specific to AAVS1 locus (left)

    Depositor

    Paul Gadue

    Article

    Sim et al Methods Mol Biol. 2015 Jan 29.

    Insert

    AAVS1-ZFNL
    

    Promoter

    PGK

    Available Since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. CROPseq-multi-EBFP2-NLS (pRW1447)

    Plasmid

    #216219

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expression

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. 20 pCOLI_A_N-His_C_STREP

    Plasmid

    #160162

    Purpose

    N-term 6xHis 3C cleavable, C--term 2xStrepII Amp resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    2xStrepII and 6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSSA-1-3

    Plasmid

    #35091

    Depositor

    David Segal

    Article

    Bhakta et al Methods Mol Biol. 2010;649:3-30.

    Insert

    Split luciferase for GZF1/3 SSA assay
    

    Expression

    Mammalian

    Mutation

    Single strand annealing (SSA) recombinaion report…

    Promoter

    SV40

    Available Since

    March 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

    Plasmid

    #51085

    Purpose

    Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV Retrograde and AAV1

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Promoter

    human Synapsin1

    Available Since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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