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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,443 results for: LEA

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Showing: 9001 - 9020 of 16443 results

  1. pAG472 H2B-iFAST

    Plasmid
    #130818
    Purpose
    Expresses H2B-iFAST in mammalian cells (nuclear labeling)
    Depositor
    Arnaud Gautier
    Insert
    H2B-iFAST
    Tags
    myc-tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    130818-plasmid-map-sequence-id-254956

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  2. pSBbi-Puro_LPAR_AKAR-WT

    Plasmid
    #125206
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-WT
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125206-plasmid-map-sequence-id-243604

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  3. 5C8G

    Plasmid
    #69968
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Tb427tmp.01.1830:MAC054-B05:C234722
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. PLXNA2

    Plasmid
    #28279
    Depositor
    Cheryl Arrowsmith
    Insert
    PLXNA2 (PLXNA2 Human)
    Tags
    His with TEV cleavage
    Expression
    Bacterial
    Mutation
    residues 1490-1600.
    Available Since
    Feb. 10, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. pcDNA3.1-hRIPK1-Flag-K599R

    Plasmid
    #112490
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    K599R
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112490-plasmid-map-sequence-id-214537

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  8. pcDNA3.1-hRIPK1-Flag-K115R

    Plasmid
    #112488
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    K115R
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112488-plasmid-map-sequence-id-214538

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  9. Top7 K39E K40E V48V K55E

    Plasmid
    #12464
    Purpose
    Plasmid contains Top7 K39E K40E V46A and K55E. Please note this is DIFFERENT than what the plasmid name implies.
    Depositor
    David Baker
    Insert
    Top7 K39E K40E V46A K55E
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    K39E K40E V46A K55E
    Available Since
    March 13, 2007
    Availability
    Academic Institutions and Nonprofits only
    12464-plasmid-map-sequence-id-235394

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  10. pX330-Flag-HeFSpCas9-H840A

    Plasmid
    #80456
    Purpose
    high fidelity enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    HeFSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Aug. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80456-plasmid-map-sequence-id-151478

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  11. Protein2PAM_NmeN4G.2

    Plasmid
    #250225
    Purpose
    Express Protein2PAM_NmeN4G.2 nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN4G.2
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 Y960H/V979I/V980I/Q981A/E1008K/M1016R/N1…
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250225-plasmid-map-sequence-id-501785

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  12. Protein2PAM_NmeN4G.4

    Plasmid
    #250227
    Purpose
    Express Protein2PAM_NmeN4G.4 nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN4G.4
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 D957G/V979I/Q981A/L990V/I1010T/G1026S/N1…
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250227-plasmid-map-sequence-id-502221

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  13. Protein2PAM_NmeN4C.1

    Plasmid
    #250218
    Purpose
    Express Protein2PAM_NmeN4C.1 nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN4C.1
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 E954C/Q981G/F1020Y/A1021S/C1023L/H1024D/…
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250218-plasmid-map-sequence-id-501771

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  14. Protein2PAM_NmeN4G.3

    Plasmid
    #250226
    Purpose
    Express Protein2PAM_NmeN4G.3 nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN4G.3
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 V979C/Q981A/I1010T/A1014K/G1026S/N1029A/…
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250226-plasmid-map-sequence-id-501764

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  15. Protein2PAM_NmeN4CNNT.2

    Plasmid
    #250223
    Purpose
    Express Protein2PAM_NmeN4CNNT.2 nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN4CNNT.2
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 I1010K/F1017L/A1021I/C1023F/H1024D/I1034…
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250223-plasmid-map-sequence-id-501763

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  16. pICSL30043

    Plasmid
    #245548
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Sv40 Nuclear Localization Signal (MASSPPKKKRKVSWKM)
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    245548-plasmid-map-sequence-id-498365

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  17. pICSL30059

    Plasmid
    #245562
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Self-Cleaving tag compatible with ProteinSelect purification system
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245562-plasmid-map-sequence-id-495558

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  18. pICSL30060

    Plasmid
    #245563
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Self-Cleaving GB1 tag compatible with ProteinSelect purification system
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245563-plasmid-map-sequence-id-495559

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  19. pICSL30061

    Plasmid
    #245564
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Self-Cleaving MBP tag compatible with ProteinSelect purification system
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245564-plasmid-map-sequence-id-495560

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  20. pICSL30044

    Plasmid
    #245549
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Nuclear Export Signal (MELALKLAGLM)
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245549-plasmid-map-sequence-id-495545

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Showing: 9001 - 9020 of 16443 results