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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,410 results for: TIM;

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Showing: 9021 - 9040 of 14410 results

  1. pJY4092

    Plasmid
    #113334
    Purpose
    Vector for Hermes transposition; Hermes TIR-flanked AvrII cut site; recoded transposase driven by TEF1 promoter
    Depositor
    Suzanne Sandmeyer
    Insert
    codon-optimized Hermes transposase
    Expression
    Yeast
    Available Since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    113334-plasmid-map-sequence-id-218285

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  2. pICSL11085_GG_Wheat_live_Cas9_for

    Plasmid
    #126881
    Purpose
    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    126881-plasmid-map-sequence-id-249630

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  3. pGEX4T1-NDFIP2

    Plasmid
    #179330
    Purpose
    E. coli optimized expression of GST-fusion of human NDFIP2
    Depositor
    Arthur Haas
    Insert
    NDFIP2 (NDFIP2 Human)
    Tags
    GST
    Expression
    Bacterial
    Available Since
    April 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    179330-plasmid-map-sequence-id-358705

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  4. FgH1tUTG_hup53_Ex4

    Plasmid
    #85538
    Purpose
    Inducible expression of guide RNA (hup53_Ex4) with fluorescent GFP reporter
    Depositor
    Marco Herold
    Insert
    hup53 Ex4
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    H1t
    Available Since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85538-plasmid-map-sequence-id-169844

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  5. FgH1tUTG_hup53_Ex5

    Plasmid
    #85539
    Purpose
    Inducible expression of guide RNA (hup53_Ex5) with fluorescent GFP reporter
    Depositor
    Marco Herold
    Insert
    hup53 Ex5
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    H1t
    Available Since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85539-plasmid-map-sequence-id-169843

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  6. pFA6a-psfGFP-kanMX6

    Plasmid
    #240551
    Purpose
    Tag S. pombe genes at 3' end (C-terminal end of protein) with superfolder GFP, codon-optimized for S. pombe
    Depositor
    Silke Hauf
    Insert
    psfGFP
    Expression
    Yeast
    Available Since
    July 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    240551-plasmid-map-sequence-id-479473

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  7. pFA6a-pTagRFP-T-kanMX6

    Plasmid
    #240555
    Purpose
    Tag S. pombe genes at 3' end (C-terminal end of protein) with TagRFP-T, codon-optimized for S. pombe
    Depositor
    Silke Hauf
    Insert
    pTagRFP-T
    Expression
    Yeast
    Available Since
    July 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    240555-plasmid-map-sequence-id-479472

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  8. pFA6a-pmNeonGreen-kanMX6

    Plasmid
    #240549
    Purpose
    Tag S. pombe genes at 3' end (C-terminal end of protein) with mNeonGreen, codon-optimized for S. pombe
    Depositor
    Silke Hauf
    Insert
    pmNeonGreen
    Expression
    Yeast
    Available Since
    July 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    240549-plasmid-map-sequence-id-479468

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  9. pFA6a-pmKate2-kanMX6

    Plasmid
    #240553
    Purpose
    Tag S. pombe genes at 3' end (C-terminal end of protein) with mKate2, codon-optimized for S. pombe
    Depositor
    Silke Hauf
    Insert
    pmKate2
    Expression
    Yeast
    Available Since
    July 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    240553-plasmid-map-sequence-id-479467

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  10. pLSU1/t35s[]:NLuc:NosT

    Plasmid
    #212180
    Purpose
    This binary vector expresses NanoLuciferase (Nluc) with the truncated 35sCAMV promoter
    Depositor
    Wayne Curtis
    Insert
    NanoLuc luciferase
    Expression
    Plant
    Mutation
    codon optimized for tomato
    Promoter
    truncated 35sCaMV
    Available Since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212180-plasmid-map-sequence-id-422749

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  11. pPB-cjOCT4-mNG-WPRE

    Plasmid
    #214123
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    mNeonGreen
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available Since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    214123-plasmid-map-sequence-id-424234

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  12. pICSL11086_GG_Wheat_live_Cas9_rev

    Plasmid
    #126882
    Purpose
    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    126882-plasmid-map-sequence-id-249649

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  13. pORFE1052

    Plasmid
    #112078
    Purpose
    Overexpression of the AtCAS9 nickase in plant under 2x35S promoter
    Depositor
    Christian Chevalier
    Insert
    AtCAS9 D10A
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Plant codon optimized nCas9 D10A S. pyogenes
    Promoter
    2x35S
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112078-plasmid-map-sequence-id-217613

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  14. pORFE0014

    Plasmid
    #112076
    Purpose
    AtCAS9 nickase D10A module CDS1ns
    Depositor
    Christian Chevalier
    Insert
    AtCAS9 D10A without stop codon
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Available Since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112076-plasmid-map-sequence-id-217621

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  15. FU-H2B-GFP-IRES-Puro

    Plasmid
    #69550
    Purpose
    Lentiviral, selectable fluorescent GFP tagging of nuclei.
    Depositor
    Charles Gersbach
    Insert
    Histone 2B - green fluorescent protein fusion
    Use
    Lentiviral
    Tags
    GFP fusion
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69550-plasmid-map-sequence-id-122943

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  16. pME-dCas9-BFP-KRAB

    Plasmid
    #221502
    Purpose
    CRISPRi machinery fish codon optimized
    Depositor
    Alessandro Ori
    Insert
    dCas9-tagBFP-KRAB
    Use
    Fish expression
    Mutation
    D10A and H839A point mutations
    Available Since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221502-plasmid-map-sequence-id-439413

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  17. CMV mRuby3-Synapsin1a

    Plasmid
    #187896
    Purpose
    For labeling pre-synaptic terminals
    Depositor
    Timothy Ryan
    Insert
    Synapsin1a
    Tags
    mRuby3
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187896-plasmid-map-sequence-id-394752

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  18. pPB-spCas9-pNeo

    Plasmid
    #214120
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    spCas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available Since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    214120-plasmid-map-sequence-id-423617

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  19. pH6-TEV-mCherry

    Plasmid
    #132430
    Purpose
    Expresses His6-tagged mCherry in E. coli using T7 promoter
    Depositor
    Mark Arbing
    Insert
    mCherry
    Tags
    N-terminal His6 tag with TEV protease site
    Expression
    Bacterial
    Mutation
    Codon optimized
    Promoter
    T7
    Available Since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    132430-plasmid-map-sequence-id-258769

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  20. pET15-EF1p_mFAP2b

    Plasmid
    #159469
    Purpose
    Encodes for EF1p_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF1p_mFAP2b
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Available Since
    Dec. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    159469-plasmid-map-sequence-id-319725

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Showing: 9021 - 9040 of 14410 results