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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,020 results for: Coli

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Showing: 9041 - 9060 of 11020 results

  1. pCJ203

    Plasmid
    #162678
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaF2-A75; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162678-plasmid-map-sequence-id-321524

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  4. pHBT-HRAS174

    Plasmid
    #248049
    Purpose
    To express His6 tag-Avi tag-TEV cleavage site-HRAS (residues 1-174) in E. coli, under the T7 promoter.
    Depositor
    Shohei Koide
    Article
    Wallon et al Proc Natl Acad Sci U S A. 2022 Oct 25;11
    Insert
    HRAS (HRAS Human)
    Tags
    His6 tag-Avi tag-TEV cleavage site N-terminal to …
    Expression
    Bacterial
    Mutation
    includes aa 1-174 only
    Promoter
    T7
    Available Since
    Nov. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    248049-plasmid-map-sequence-id-493231

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  6. 7AA-aSyn-pRK172

    Plasmid
    #236197
    Purpose
    This plasmid expresses human α-synuclein with a 7-amino acid insertion (MAAAEKT) in the pRK172 backbone for bacterial expression in E. coli. The insertion corresponds to the JOS mutation.
    Depositor
    Sjors Scheres, Yang Yang
    Article
    Yang et al Acta Neuropathol. 2023 May;145(5):561-57
    Insert
    SNCA (SNCA Human)
    Expression
    Bacterial
    Mutation
    insertion os MAAAEKT at residue 22
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    236197-plasmid-map-sequence-id-469796

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  12. pMBP-HsSyx3

    Plasmid
    #179287
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Tags
    His6 + mature maltose binding protein + TEV prote…
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179287-plasmid-map-sequence-id-354925

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  13. pMBP-HsSyx-375-792

    Plasmid
    #179291
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Tags
    His6 + mature maltose binding protein + TEV prote…
    Expression
    Bacterial
    Mutation
    residues 375-792 from accession number NP_0010361…
    Promoter
    T7
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179291-plasmid-map-sequence-id-354915

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Showing: 9041 - 9060 of 11020 results