Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 10,540 results for: HAE

Showing: 9041 - 9060 of 10540 results

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pET28a-anticalin (N116amb)-His

Plasmid

#168045

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Asn 116 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (N21amb)-His

Plasmid

#168041

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Asn 21 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Fgβ-anticalin-His-ybbr

Plasmid

#168040

Purpose

Expresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminus

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag, Fgβ peptide, and ybbr tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (I55amb)-His

Plasmid

#168042

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Ile 55 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 ΔH1 Δ5H

Plasmid

#163582

Purpose

express Dcp2 ΔH1 Δ5H at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

L255A; mutate 440-450, 489-500, 701-708, 827-831,…

Promoter

DCP2 promoter

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2C Δ5H

Plasmid

#163581

Purpose

express Dcp2C Δ5H at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Truncation of aa1-300; mutate aa440-450, aa489-50…

Promoter

DCP2 promoter

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Coh-ddFLN4-ELP(MV7E2)3-His-ybbR

Plasmid

#157672

Purpose

Expression of Coh (type III from Ruminococcus flavefaciens) with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

Depositor

Michael Nash

Article

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

Cohesin (R.f) with ddFLN4, ELP, Histag and ybbR tag

Tags

ddFLN4, ELP, Histag and ybbR tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159961

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159965

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

CMV-IE

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159964

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

CMV-IE

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-mTurquoise2-optoJNKi-V416I-WPRE

Plasmid

#159970

Purpose

optogenetic JNK inhibitor with extended relaxation time

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mTurquoise2-optoJNKi-V416I

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-mTurquoise2dC8-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159958

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC8-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159957

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159960

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-Synapsin-NLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159959

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-CMV-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159963

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

CMV-IE

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-mScarlet-optoJNKi-WPRE

Plasmid

#159968

Purpose

optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mScarlet-optoJNKi

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-YPET-optoNES.F434L-WPRE

Plasmid

#159975

Purpose

optical regulation of nuclear export with shorter relaxation time

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-YPET-optoNES.F434L

Use

AAV

Expression

Mammalian

Mutation

F434L

Promoter

hSyn1

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-YPET-optoNES.V416I-WPRE

Plasmid

#159973

Purpose

optical regulation of nuclear export with extended relaxation time

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-YPET-optoNES.V416I

Use

AAV

Expression

Mammalian

Mutation

V416I

Promoter

hSyn1

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-YPET-optoNES.I427V-WPRE

Plasmid

#159974

Purpose

optical regulation of nuclear export with shorter relaxation time

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-YPET-optoNES.I427V

Use

AAV

Expression

Mammalian

Mutation

I427V

Promoter

hSyn1

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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Showing: 9041 - 9060 of 10540 results

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