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pLKO.1P BRIP1_1

Plasmid

#160771

Purpose

Suppress BRIP1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shBRIP1_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1B CDC25C_2

Plasmid

#160770

Purpose

Suppress CDC25C

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCDC25C_2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1B CDC25C_2

Plasmid

#160770

Purpose

Suppress CDC25C

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCDC25C_2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1B CDC25C_1

Plasmid

#160769

Purpose

Suppress CDC25C

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCDC25C_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1B CDC25C_1

Plasmid

#160769

Purpose

Suppress CDC25C

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCDC25C_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1B CDC25A_1

Plasmid

#160767

Purpose

Suppress CDC25A

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCDC25A_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1B CDC25A_1

Plasmid

#160767

Purpose

Suppress CDC25A

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCDC25A_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pMTTH+LANA-H1.4CTD

Plasmid

#157798

Purpose

Expression of LANA peptide-human linker histone H1.4 CTD fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

LANA-H1.4CTD (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pMTTH+LANA-H1.4CTD

Plasmid

#157798

Purpose

Expression of LANA peptide-human linker histone H1.4 CTD fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

LANA-H1.4CTD (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINT4

Plasmid

#127513

Purpose

Plasmid encodes H. sapiens codon optimized Integrase 4.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 4 coding sequence codon optimized for H. sapiens expression.

Expression

Mammalian

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINT5

Plasmid

#127514

Purpose

Plasmid encodes H. sapiens codon optimized Integrase 5.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 5 coding sequence codon optimized for H. sapiens expression.

Expression

Mammalian

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINT4

Plasmid

#127513

Purpose

Plasmid encodes H. sapiens codon optimized Integrase 4.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 4 coding sequence codon optimized for H. sapiens expression.

Expression

Mammalian

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINT5

Plasmid

#127514

Purpose

Plasmid encodes H. sapiens codon optimized Integrase 5.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 5 coding sequence codon optimized for H. sapiens expression.

Expression

Mammalian

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-evoSpCas9 (without sgRNA; with silent mutations)

Plasmid

#126758

Purpose

Expression plasmid for human codon-optimized increased fidelity evoSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

evoSpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

M495V, Y515N, K526E, R661Q

Promoter

CBh

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-HeFSpCas9 (without sgRNA; with silent mutations)

Plasmid

#126759

Purpose

Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

HeFSpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

Promoter

CBh

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-evoSpCas9 (without sgRNA; with silent mutations)

Plasmid

#126758

Purpose

Expression plasmid for human codon-optimized increased fidelity evoSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

evoSpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

M495V, Y515N, K526E, R661Q

Promoter

CBh

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-HeFSpCas9 (without sgRNA; with silent mutations)

Plasmid

#126759

Purpose

Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

HeFSpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

Promoter

CBh

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-eSpCas9 (without sgRNA; with silent mutations)

Plasmid

#126754

Purpose

Expression plasmid for human codon-optimized increased fidelity eSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

eSpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

K848A, K1003A, R1060A

Promoter

CBh

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-HypaSpCas9 (without sgRNA; with silent mutations)

Plasmid

#126756

Purpose

Expression plasmid for human codon-optimized increased fidelity HypaSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

HypaSpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N692A, M694A, Q695A, H698A

Promoter

CBh

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-eSpCas9 (without sgRNA; with silent mutations)

Plasmid

#126754

Purpose

Expression plasmid for human codon-optimized increased fidelity eSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

eSpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

K848A, K1003A, R1060A

Promoter

CBh

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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