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p3E-rab27a

Plasmid

#109685

Purpose

Multisite gateway vector for 3' tagging with rab27a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab27a (rab27a Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBW1311_pCAG-PV1-ABI-L1-CTD-ENTRY-BGHpA

Plasmid

#108839

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to ABI tag on N-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

ABI

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP47ts-2A(sfGFP)

Plasmid

#126992

Purpose

Level 2 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

RepA A56V thermosensitive mutation

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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IO901: pMVP (L5-L4) BioID2

Plasmid

#121724

Purpose

pMVP L5-L4 entry plasmid, contains BioID2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term BioID2 to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

BioID2 (open)

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IG401: pMVP (L1-R5) RIP

Plasmid

#121694

Purpose

pMVP L1-R5 entry plasmid, contains rat Ins1 promoter (RIP) for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

RIP

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KU701: pMVP (L5-L4) eGFP

Plasmid

#121707

Purpose

pMVP L5-L4 entry plasmid, contains eGFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eGFP fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eGFP (open)

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMK-S. tuberosum trnI/trnA

Plasmid

#123583

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

S. tuberosum trnI/trnA integration site

Use

Chloroplast transformation vector

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP27ts-1A(lacZ)

Plasmid

#126977

Purpose

Level 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

RepA A56V thermosensitive mutation

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-3xflagGREB1c

Plasmid

#112223

Purpose

mammalian expression of flag tagged GREB1c

Depositor

Craig Burd

Article

Haines et al Endocr Relat Cancer. 2018 Jul;25(7):735-

Insert

GREB1c

Tags

3x flag

Expression

Mammalian

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01*(ΔL1+L2)

Plasmid

#80044

Purpose

Expression of EPN-01* mutated in both L domains

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*(L33R)

Tags

Myc

Expression

Mammalian

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pJK544

Plasmid

#72517

Purpose

Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)

Depositor

T. J. Kappock

Insert

formylglycinamidine ribonucleotide synthetase, small subunit

Expression

Bacterial

Mutation

contains additional Ala2

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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RASL11B

Plasmid

#55678

Purpose

expression of mouse RASL11B

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RASL11B (Rasl11b Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
RALB

Plasmid

#55658

Purpose

expression of mouse RALB

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RALB (Ralb Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pK812

Plasmid

#44622

Depositor

Robert Oshima

Article

Kulesh et al Mol Cell Biol. 1989 Apr;9(4):1553-65.

Insert

K8 (KRT8 Human)

Use

Cloning

Available Since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

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pHEX2 QQR ZFN

Plasmid

#44463

Depositor

Roger Hellens, Ross Johnson

Insert

QQR ZFN

Use

Plant transformation

Promoter

CaMV 35S

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only
TFORF1372

Plasmid

#143953

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF143 (ZNF143 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF1876

Plasmid

#143858

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CCNT1 (CCNT1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pRP.USH2A

Plasmid

#226555

Purpose

Minigene encoding USH2A exon 13 and surrounding introns/exons

Depositor

Dave Wee

Article

Tabaglio et al Mol Ther Nucleic Acids. 2025 Jan 7;36(1)

Insert

USH2A (USH2A Human)

Expression

Mammalian

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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MCP/PCP red RNA Lantern

Plasmid

#226097

Purpose

RNA tracking

Depositor

Jennifer Prescher

Article

Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

Insert

NLS-HA-MS2-SmBiT-IRES-PP7-mScarlet-I-LgBiT-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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actb2-SecAnxaV-mKate2-acry-EGFP

Plasmid

#123628

Purpose

Ubiquitous zebrafish expression vector containing secreted human AnnexinV tagged to mKate2. Parton lab clone BDN.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

AnnexinA5

Tags

mKate2

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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