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pcDNA3-CARMA2sh-delta458-500

Plasmid

#97074

Purpose

Expresses mutant CARD14/CARMA2 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

Insert

CARD14 isoform 3 (CARD14 Human)

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 458-500

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-2B-Nkx2-5 Y191C

Plasmid

#98613

Purpose

Gateway entry vector for NKX2-5 Y191C

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

NKX2-5 Y191C (Nkx2-5 Mouse)

Use

Entry vector

Mutation

Point mutation Y191C

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-ELK1Y158A

Plasmid

#98628

Purpose

Gateway entry TOPO TA cloning vector ELK1 Y158A

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ELK1 Y158A (Elk1 Mouse)

Use

Topo ta cloning vector

Mutation

Y158A

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAav-IQGAP1-PQS1-3xFLAG

Plasmid

#84885

Purpose

rAAV-based template for genome engineering of the IQGAP1 C-terminus containing PQS1 and 3xFLAG tags and a selection cassette

Depositor

Sven Eyckerman

Article

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Inserts

IQGAP1 genomic region HR1 (IQGAP1 Human)
IQGAP1 genomic region HR2

Use

AAV

Tags

PQS1 3xFLAG

Promoter

no

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAav-TP53-PQS1-3xFLAG

Plasmid

#84884

Purpose

rAAV-based template for genome engineering of the p53 C-terminus containing PQS1 and 3xFLAG tags and a selection cassette

Depositor

Sven Eyckerman

Article

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Inserts

TP53 genomic region HR1 (TP53 Human)
TP53 genomic region HR2

Use

AAV

Tags

PQS1 3xFLAG

Promoter

no and no

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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LAMTOR3 gRNA (BRDN0001147669)

Plasmid

#77136

Purpose

3rd generation lentiviral gRNA plasmid targeting human LAMTOR3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LAMTOR3 (LAMTOR3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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CROT gRNA (BRDN0001145682)

Plasmid

#77709

Purpose

3rd generation lentiviral gRNA plasmid targeting human CROT

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CROT (CROT Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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KALRN gRNA (BRDN0001145005)

Plasmid

#77639

Purpose

3rd generation lentiviral gRNA plasmid targeting human KALRN

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

KALRN (KALRN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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NPR2 gRNA (BRDN0001148355)

Plasmid

#77753

Purpose

3rd generation lentiviral gRNA plasmid targeting human NPR2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NPR2 (NPR2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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CAMK2B gRNA (BRDN0001148102)

Plasmid

#77742

Purpose

3rd generation lentiviral gRNA plasmid targeting human CAMK2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CAMK2B (CAMK2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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AURKA gRNA (BRDN0001145351)

Plasmid

#77728

Purpose

3rd generation lentiviral gRNA plasmid targeting human AURKA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AURKA (AURKA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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CDK5R1 gRNA (BRDN0001145710)

Plasmid

#77851

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK5R1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK5R1 (CDK5R1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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IPMK gRNA (BRDN0001146121)

Plasmid

#78075

Purpose

3rd generation lentiviral gRNA plasmid targeting human IPMK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

IPMK (IPMK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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C17orf75 gRNA (BRDN0001145175)

Plasmid

#78077

Purpose

3rd generation lentiviral gRNA plasmid targeting human C17orf75

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

C17orf75 (C17orf75 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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C17orf75 gRNA (BRDN0001146368)

Plasmid

#78078

Purpose

3rd generation lentiviral gRNA plasmid targeting human C17orf75

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

C17orf75 (C17orf75 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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C17orf75 gRNA (BRDN0001487133)

Plasmid

#78079

Purpose

3rd generation lentiviral gRNA plasmid targeting human C17orf75

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

C17orf75 (C17orf75 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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LRGUK gRNA (BRDN0001145468)

Plasmid

#78084

Purpose

3rd generation lentiviral gRNA plasmid targeting human LRGUK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LRGUK (LRGUK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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LRGUK gRNA (BRDN0001147114)

Plasmid

#78085

Purpose

3rd generation lentiviral gRNA plasmid targeting human LRGUK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LRGUK (LRGUK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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LRGUK gRNA (BRDN0001487051)

Plasmid

#78086

Purpose

3rd generation lentiviral gRNA plasmid targeting human LRGUK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LRGUK (LRGUK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AURKC gRNA (BRDN0001146500)

Plasmid

#78054

Purpose

3rd generation lentiviral gRNA plasmid targeting human AURKC

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AURKC (AURKC Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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