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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,278 results for: nes

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Showing: 9161 - 9180 of 16278 results

  2. pPyCAG-HA-EGFP-Myc-TM-IRES-Pac

    Plasmid
    #183605
    Purpose
    Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance.
    Depositor
    Sally Lowell
    Publication
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-TM
    Tags
    HA, Human PDGFRB transmembrane domain, Mouse IgGK…
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183605-plasmid-map-sequence-id-361591

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  4. pLJM1-Flag-Rheb-4Rs

    Plasmid
    #165022
    Purpose
    expression of Flag-Rheb-4Rs mutant protein in mammalian cells (the lysine residues on the position 109, 135, 151 and 178 on Rheb protein are mutated to arginines)
    Depositor
    Ken Inoki
    Publication
    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d
    Insert
    Rheb-4Rs (RHEB Human)
    Use
    Lentiviral
    Tags
    flag
    Expression
    Mammalian
    Mutation
    change lysine residues on position 109, 135, 151,…
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165022-plasmid-map-sequence-id-326717

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  5. pCEP4-SERTG56A -myc

    Plasmid
    #107459
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G56A conferring wildtype phenotype
    Depositor
    Erik Procko
    Publication
    Young et al bioRxiv 2021.04.19.440442
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    G56A
    Promoter
    CMV
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    107459-plasmid-map-sequence-id-205554

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  8. CΔ4

    Plasmid
    #74299
    Purpose
    Mammalian expression of treacle (residues 1271-1302) fused to GFP
    Depositor
    Rita Shiang
    Publication
    Winokur et al Hum Mol Genet. 1998 Nov;7(12):1947-52.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    treacle ribosome biogenesis factor 1 (Tcof1 Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Expresses residues 1271 - 1302
    Promoter
    CMV
    Available since
    Aug. 8, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. pTXB1-hMeCP2-G273X

    Plasmid
    #48093
    Purpose
    Bacterial expression of Mxe intein/chitin binding domain tagged MeCP2-G273X
    Depositor
    Huda Zoghbi
    Publication
    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10
    Insert
    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    Expression
    Bacterial
    Mutation
    Mxe intein/chitin binding domain tagged, expresse…
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pTB107-Hyg

    Plasmid
    #236781
    Purpose
    Expression of Cas9 cytosine base editor (CBE), T7 RNAP, Cas12a and hygromycin selection marker. The CBE contains a ssDNA-DBD from the H. sapiens RAD51 protein. This plasmid is transfected as episome.
    Depositor
    Tom Beneke
    Publication
    Herrmann May et al eLife. 2025 Feb 24;13:RP97437. doi: 10.7
    Insert
    hyBE4max (CBE); Cas12a (Acidaminococcus sp. BV3L6)-P2A-T7 RNAP
    Use
    CRISPR
    Available since
    April 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    236781-plasmid-map-sequence-id-505275

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  13. pSA097 HNRNPK_IVS3-Cas9-BFP

    Plasmid
    #249147
    Purpose
    Overexpression of SpCas9-BFP with HNRNPK IVS3 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Publication
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HNRNPK_IVS3-Cas9-TagBFP (HNRNPK S. pyogenes Cas9, synthetic, Human)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HNRNPK IVS3 intron
    Promoter
    EF1a
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249147-plasmid-map-sequence-id-496390

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  14. VEE-mScarlet3-IRES-E3-NSs-L*-EGFP-IRES-srIκBα

    Plasmid
    #242411
    Purpose
    Self-amplifying RNA (saRNA) construct expresses dsRNA pathway inhibitors (E3, NSs, L*) and inflammatory signaling inhibitor, super repressor inhibitor of κBα (srIκBα).
    Depositor
    Ewan St. John Smith
    Publication
    Lim et al bioRxiv 2024.09.24.614636
    Inserts
    mScarlet3
    IRES-E3-NSs-L*-EGFP
    IRES-srIκBα
    Use
    Mouse Targeting; Self-amplifying rna
    Expression
    Mammalian
    Mutation
    serines 32 and 36 to alanines
    Available since
    Sept. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    242411-plasmid-map-sequence-id-487192

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  15. pYN2_1

    Plasmid
    #184757
    Purpose
    Plasmid for genome editing by CRISPR/Cas9
    Depositor
    Yvonne Nygård
    Publication
    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10
    Inserts
    Cas9
    sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
    Use
    Synthetic Biology
    Tags
    2xNLS
    Expression
    Bacterial and Yeast
    Promoter
    PGK1 and tRNA-Phe-HDV Ribozyme
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    184757-plasmid-map-sequence-id-421882

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  16. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid
    #176264
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Publication
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176264-plasmid-map-sequence-id-347050

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Showing: 9161 - 9180 of 16278 results