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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 9161 - 9180 of 11091 results

  2. pR6Kg-Omega2

    Plasmid
    #165865
    Purpose
    Omega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165865-plasmid-map-sequence-id-335016

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  5. pJK526

    Plasmid
    #155387
    Purpose
    PphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA4 in dCas12a oscillator crRNAs
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    155387-plasmid-map-sequence-id-309008

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  7. pBP-ORF

    Plasmid
    #72949
    Purpose
    Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 0 - ORFs
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72949-plasmid-map-sequence-id-140106

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  12. pVH028

    Plasmid
    #80393
    Purpose
    Contains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains + salicylate inducible phosphatase (Psal-CusSmut)
    Depositor
    Richard Murray
    Article
    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.
    Inserts
    Taz chimeric histine kinase
    CusS (cusS )
    NahR (nahR )
    Use
    Synthetic Biology
    Tags
    Fused by GS linkers to 4 SH3 domains
    Expression
    Bacterial
    Mutation
    Glycine 448 changed to Alanine, makes it primaril…
    Available Since
    Nov. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    80393-plasmid-map-sequence-id-166533

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  17. pSBL_DK198

    Plasmid
    #156425
    Purpose
    Insect cell expression of 3a delta N(aa 42-275)-sfGFP, needs to be introduced into baculoviral bacmid
    Depositor
    Stephen Brohawn
    Article
    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.
    Insert
    SARS-CoV-2 3a protein (aa 42-275)
    Tags
    sfGFP-His
    Expression
    Insect
    Mutation
    aa 42-275
    Promoter
    Polyhedrin
    Available Since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    156425-plasmid-map-sequence-id-307748

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  20. pAGW-dpse-GAL4-DBD

    Plasmid
    #125153
    Purpose
    Gateway-compatible vector to express a fusion protein containing the GAL4-DBD (N-terminally) followed by the protein of interest under the control of a D.pseudoobscura enhancer and CP
    Depositor
    Alexander Stark
    Article
    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:
    Insert
    D. pseudoobscura peak #102 enhancer and D. pseudoobscura CG13116 CP
    Use
    Gateway fly expression vector
    Expression
    Insect
    Available Since
    Aug. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    125153-plasmid-map-sequence-id-248016

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Showing: 9161 - 9180 of 11091 results