We narrowed to 9,345 results for: yeast expression

1. pPICZ-His6-K2P4.1B-mCherry-StreptagII

   Plasmid

   #158744

   Purpose

   Expresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.

   Depositor

   Arthur Laganowsky

   Article

   Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi

   Insert

   K2P4.1-B (KCNK4 Human)
   

   Tags

   His6 and mCherry with Streptag II

   Expression

   Yeast

   Mutation

   Residues 1-290 and N-linked glycosylation sites r…

   Promoter

   AOX1

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. p426_Cas9_gRNA-R-ARS805a

   Plasmid

   #87408

   Purpose

   p426_Cas9_gRNA-ARS805a without ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS805a sequence TTATTTGAATGATATTTAGT in yeast chromosome 8.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS805a
   

   Use

   CRISPR

   Promoter

   ADH1, pTyrosine

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBSWHhc-Lam

   Plasmid

   #133902

   Purpose

   Negative control when used in combination with pAWH-largeT. Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Insert

   LMNA (Lam) (LMNA Human)
   

   Expression

   Yeast

   Promoter

   T7

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEN-L1-K2-L2

   Plasmid

   #80684

   Purpose

   Gateway Entry vector containing a temperature-sensitive mouse DHFR

   Depositor

   Nico Dissmeyer

   Article

   Faden et al Nat Commun. 2016 Jul 22;7:12202. doi: 10

   Inserts

   DHFR (Dhfr Mouse)
   DHFR (Dhfr Mouse, Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   3xHA

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   mouse DHFR with N-terminal Ubiquitin

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCHKU38.2-2.2

   Plasmid

   #110670

   Purpose

   Expression of a fungal biosynthetic gene cluster

   Depositor

   Ronald Davis

   Article

   Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

   Insert

   S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6, ICL1-ORF7
   

   Expression

   Yeast

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCHSU74.2-2.2

   Plasmid

   #115543

   Purpose

   Expression of a fungal biosynthetic gene cluster

   Depositor

   Ronald Davis

   Article

   Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

   Insert

   S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6, ICL1-ORF7
   

   Expression

   Yeast

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCHKU16.1-2.2

   Plasmid

   #115529

   Purpose

   Expression of a fungal biosynthetic gene cluster

   Depositor

   Ronald Davis

   Article

   Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

   Insert

   S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5
   

   Expression

   Yeast

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCHKU20.1-2.2

   Plasmid

   #115531

   Purpose

   Expression of a fungal biosynthetic gene cluster

   Depositor

   Ronald Davis

   Article

   Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

   Insert

   S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6
   

   Expression

   Yeast

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCHSU68.2-2.2

   Plasmid

   #110681

   Purpose

   Expression of a fungal biosynthetic gene cluster

   Depositor

   Ronald Davis

   Article

   Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

   Insert

   S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6
   

   Expression

   Yeast

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCHKU27.2-2.2

    Plasmid

    #110662

    Purpose

    Expression of a fungal biosynthetic gene cluster

    Depositor

    Ronald Davis

    Article

    Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

    Insert

    S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5
    

    Expression

    Yeast

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCHSU75.2-2.2

    Plasmid

    #110691

    Purpose

    Expression of a fungal biosynthetic gene cluster

    Depositor

    Ronald Davis

    Article

    Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

    Insert

    S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5
    

    Expression

    Yeast

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCHKU33.2-2.2

    Plasmid

    #110667

    Purpose

    Expression of a fungal biosynthetic gene cluster

    Depositor

    Ronald Davis

    Article

    Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

    Insert

    S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6
    

    Expression

    Yeast

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPICZ A-SLC-His+MFN2-Strep (SB255)

    Plasmid

    #227604

    Purpose

    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Inserts

    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    

    Tags

    10xHis and Twin-StrepTag

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIRHD-TetR-GAL80-Rho1-GAL4

    Plasmid

    #218274

    Purpose

    Introduction of low-temperature-inducible GAL system, LowTempGAL v2.2

    Depositor

    Christopher Howard

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    gal80Arm1-pAgTEF1>KanMX4>tAgTEF1-pHAC1>UBI4>>DHFRP66L-TetR>NLS>TUP1>tABF1-pTEF2>Rho1C17R>>GAL4>tGAL4-pTEF1(TetO)>GAL80Arm2
    

    Expression

    Yeast

    Mutation

    DHFR (P66L), Rho1 (C17R)

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIRHDGAL43C

    Plasmid

    #218279

    Purpose

    Introduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter induction

    Depositor

    Claudia Vickers

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)
    

    Expression

    Yeast

    Mutation

    GAL3 (S509P), DHFR (P66L)

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIRGAL43C

    Plasmid

    #218278

    Purpose

    Introduction of a LowTempGAL Turbo module (GAL43C) to accelerate induction of GAL promoter induction

    Depositor

    Claudia Vickers

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
    

    Expression

    Yeast

    Mutation

    GAL3 (S509P)

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIRHDGAL3C

    Plasmid

    #218276

    Purpose

    Introduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter induction

    Depositor

    Claudia Vickers

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
    

    Expression

    Yeast

    Mutation

    GAL3 (S509P), DHFR (P66L)

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIRGAL3C

    Plasmid

    #218275

    Purpose

    Introduction of a LowTempGAL Turbo module (GAL3C) to accelerate induction of GAL promoter induction

    Depositor

    Claudia Vickers

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
    

    Expression

    Yeast

    Mutation

    GAL3 (S509P)

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pXYL-crtI-tNOS-pGPD-crtYB-tNOS+ku70 insD (SBE146)

    Plasmid

    #195048

    Purpose

    cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Inserts

    crtE
    crtI
    crtYB
    

    Expression

    Yeast

    Promoter

    pADH2, pGPD, and pXYL

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pGPD-crtI-tNOS-pXYL-crtYB-tNOS+ku70 insD (SBE145)

    Plasmid

    #195047

    Purpose

    cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Inserts

    crtE
    crtI
    crtYB
    

    Expression

    Yeast

    Promoter

    pADH2, pGPD, and pXYL

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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