Skip to main content
Addgene
Showing: 1351 - 1380 of 31301 results

  1. pIRTetR-GAL80

    Plasmid
    #185853
    Purpose
    Plugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiae
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    gal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185853-plasmid-map-sequence-id-370762

    Enlarge

  2. pILGFP4D41

    Plasmid
    #185841
    Purpose
    Testing the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185841-plasmid-map-sequence-id-369628

    Enlarge

  4. pILGFP8EFA

    Plasmid
    #185854
    Purpose
    Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    DHFR P66L
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185854-plasmid-map-sequence-id-370766

    Enlarge

  6. pcS-E626-C1C2

    Plasmid
    #200162
    Purpose
    This plasmid encodes EGFR targeting monobody (E626) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.
    Depositor
    Masako Harada
    Article
    Komuro et al Bioengineering (Basel). 2022 Jan 29;9(2)
    Inserts
    E626
    C1C2 domain of lactadherin
    Use
    Tags
    HA and HIS
    Expression
    Mammalian
    Mutation
    sequence adopted from doi: 10.1371/journal.pone.0…
    Promoter
    CMV
    Available since
    June 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    200162-plasmid-map-sequence-id-396334

    Enlarge

  7. pcS-RDG-C1C2

    Plasmid
    #200163
    Purpose
    This plasmid encodes non-binder control monobody (RDG) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.
    Depositor
    Masako Harada
    Article
    Komuro et al Bioengineering (Basel). 2022 Jan 29;9(2)
    Inserts
    RDG-C1C2
    C1C2 domain of lactadherin
    Use
    Tags
    HA and HIS
    Expression
    Mammalian
    Mutation
    sequence adopted from doi: 10.1371/journal.pone.0…
    Promoter
    Available since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    200163-plasmid-map-sequence-id-409210

    Enlarge

  12. pGS-CD64-2

    Plasmid
    #109191
    Purpose
    Encodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression system
    Depositor
    Monika Golas
    Article
    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do
    Insert
    Full-length engineered CD64, derived from human CD64
    Use
    Tags
    Twin-Strep
    Expression
    Insect
    Mutation
    engineered for efficient expression, see publicat…
    Promoter
    Polyhedrin
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    109191-plasmid-map-sequence-id-233794

    Enlarge

  18. pGGC_RT_mScarlet

    Plasmid
    #136967
    Purpose
    Goldengate/Greengate CDS module, containing mScarlet
    Depositor
    Klaus Theres
    Insert
    mScarlet
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136967-plasmid-map-sequence-id-269105

    Enlarge

  19. pGGD_RT_SRDX

    Plasmid
    #136976
    Purpose
    Goldengate/Greengate module EAR-repression domain
    Depositor
    Klaus Theres
    Insert
    SRDX
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    136976-plasmid-map-sequence-id-272093

    Enlarge

  20. pGGC_RT_Tq2CFP

    Plasmid
    #136969
    Purpose
    Goldengate/Greengate CDS module, containing Tq2CFP.
    Depositor
    Klaus Theres
    Insert
    Tq2CFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    136969-plasmid-map-sequence-id-270557

    Enlarge

  21. pGGD_RT_2NLS

    Plasmid
    #136977
    Purpose
    Goldengate/Greengate module nuclear localization signal
    Depositor
    Klaus Theres
    Insert
    SV40 NLS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136977-plasmid-map-sequence-id-269089

    Enlarge

  22. pGGA_RT_pLexA

    Plasmid
    #136961
    Purpose
    Goldengate/Greengate module for generation of beta-estradiole inducible genes.
    Depositor
    Klaus Theres
    Insert
    pLexA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136961-plasmid-map-sequence-id-269088

    Enlarge

  23. pGGD_RT_li_Tq2CFP

    Plasmid
    #136971
    Purpose
    Goldengate/Greengate C-tag module, containing Tq2CFP fused to a flexible linker
    Depositor
    Klaus Theres
    Insert
    Tq2CFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136971-plasmid-map-sequence-id-269106

    Enlarge

  24. pGGB_RT_Tq2CFP_li

    Plasmid
    #136965
    Purpose
    Goldengate/Greengate N-tag module, containing Tq2CFP fused to a flexible linker
    Depositor
    Klaus Theres
    Insert
    Tq2CFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136965-plasmid-map-sequence-id-269100

    Enlarge

  25. pGGB_RT_Venus_li

    Plasmid
    #136966
    Purpose
    Goldengate/Greengate N-tag module, containing Venus fused to a flexible linker
    Depositor
    Klaus Theres
    Insert
    Venus
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136966-plasmid-map-sequence-id-269102

    Enlarge

  26. pGGB_RT_tdTomato_li

    Plasmid
    #136964
    Purpose
    Goldengate/Greengate N-tag module, containing tdTomato fused to a flexible linker
    Depositor
    Klaus Theres
    Insert
    tandem Tomato
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136964-plasmid-map-sequence-id-269098

    Enlarge

  27. pGGC_RT_Sa_sgRNA

    Plasmid
    #136968
    Purpose
    Goldengate/Greengate module for genome editing, using the Sa guideRNA.
    Depositor
    Klaus Theres
    Insert
    Sa guide RNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136968-plasmid-map-sequence-id-269077

    Enlarge

  28. pGGI_RT_Sa_sgRNA

    Plasmid
    #136982
    Purpose
    Goldengate/Greengate module for genome editing, using the Sa guideRNA.
    Depositor
    Klaus Theres
    Insert
    Sa guide RNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    136982-plasmid-map-sequence-id-270593

    Enlarge

  29. pGGD_RT_Sa_sgRNA

    Plasmid
    #136975
    Purpose
    Goldengate/Greengate module for genome editing, using the Sa guideRNA.
    Depositor
    Klaus Theres
    Insert
    Sa guide RNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    136975-plasmid-map-sequence-id-270568

    Enlarge

  30. pGGD_RT_li_Tq2CFP_2NLS

    Plasmid
    #136972
    Purpose
    Goldengate/Greengate C-tag module, containing Tq2CFP fused to 2 SV40 NLS and a flexible linker
    Depositor
    Klaus Theres
    Insert
    Tq2CFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136972-plasmid-map-sequence-id-269108

    Enlarge

Showing: 1351 - 1380 of 31301 results