We narrowed to 17,553 results for: FIE

1. pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

   Plasmid

   #67983

   Purpose

   Cas9 activity reporter (control) with GFP and BFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. anti-Jagged_D5-pBIOCAM5

   Plasmid

   #39345

   Depositor

   John McCafferty

   Article

   Schofield et al Genome Biol. 2007;8(11):R254.

   Insert

   scFv specific for Jagged (clone D5) (JAG1 Human)
   

   Use

   Tags

   3xFLAG and 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-NES-ZapCV2

   Plasmid

   #222950

   Purpose

   Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

   Depositor

   Amy Palmer

   Article

   Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

   Insert

   NES-ZapCV2 (NES Synthetic)
   

   Use

   Tags

   ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…

   Expression

   Mammalian

   Mutation

   ECFP gene is truncated 36 bp at 3' end. Venu…

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS2 (CK2alpha', CK2beta)

   Plasmid

   #27093

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Inserts

   CK2beta (CSNK2B Human)
   CK2alpha' (CSNK2A2 Human)
   

   Use

   Tetracycline-regulated expression

   Tags

   HA and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   bidirectional tet-responsive promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGV13 (CK2alpha K68M, CK2beta)

   Plasmid

   #27094

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Inserts

   CK2beta (CSNK2B Human)
   CK2alpha K68M (CSNK2A1 Human)
   

   Use

   Tetracycline-regulated expression

   Tags

   HA on CK2alpha and Myc on CK2beta

   Expression

   Mammalian

   Mutation

   K68M on CK2alpha

   Promoter

   bidirectional tet-responsive promoter and bidirec…

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGV7 (CK2alpha' K69M, CK2beta)

   Plasmid

   #27095

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Inserts

   CK2beta (CSNK2B Human)
   CK2alpha' K69M (CSNK2A2 Human)
   

   Use

   Tetracycline-regulated expression

   Tags

   HA tag on CK2alpha' and Myc on CK2beta

   Expression

   Mammalian

   Mutation

   K69M on CK2alpha'

   Promoter

   bidirectional tet-responsive promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcmCherry 3.1(-)

   Plasmid

   #62803

   Purpose

   Modified pcDNA3.1(-), with Neomycin resistance cassette replaced by mCherry

   Depositor

   Michal Minczuk

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET283xFlagPP7CPHis

   Plasmid

   #28174

   Depositor

   Kathleen Collins

   Insert

   PP7 coat protein optimized
   

   Use

   Tags

   6xhistidine and triple FLAG peptide

   Expression

   Bacterial

   Mutation

   C68A,C73A

   Promoter

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. NLS-tdTomato

   Plasmid

   #163443

   Purpose

   Bacterial expression of His-tagged, SV40 NLS-tagged tdTomato in the modified pRSETB protein expression backbone

   Depositor

   Sanford Simon

   Insert

   tdTomato
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW57.1_blasti

    Plasmid

    #194067

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW57.1_TStrp_HA_Ct

    Plasmid

    #194066

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Twin_Strep-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57.1_TStrp_HA_Nt

    Plasmid

    #194065

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    HA-Twin_Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCW57.1_V5_FLAG_Ct

    Plasmid

    #194075

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FLAG-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW57.1_V5_FLAG_Nt

    Plasmid

    #194074

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSRKBB-empty

    Plasmid

    #59449

    Purpose

    Contains BioBrick prefixed and suffixed insert with a modified lac promoter, Shine-Delgarno sequence, 5’ & 3’ MCS, 3’ 6xHis tag.

    Depositor

    Claudia Schmidt-dannert

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    modified lac

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. T15m

    Plasmid

    #121034

    Purpose

    MoClo golden gate assembly DE part for L3S3P11 (173x-strength synthetic terminator, modified to remove BsaI cut site; see DOI: 10.1038/nmeth.2515).

    Depositor

    Richard Murray

    Insert

    modified L3S3P11 (synthetic Voigt terminator)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCW57.1_V5_TurboID_FLAG_Nt

    Plasmid

    #194072

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5-TurboID-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW57.1_V5_TurboID_FLAG_Ct

    Plasmid

    #194073

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FLAG-TurboID-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCW57.1_V5_APEX_FLAG_Nt

    Plasmid

    #194068

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5-APEX-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCW57.1_V5_APEX_FLAG_Ct

    Plasmid

    #194069

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FLAG-APEX-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge