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pAW167

Plasmid

#113187

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 R40E

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R40E

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW156

Plasmid

#113185

Purpose

E. coli expression vector for untagged E. coli Cas1 K259E + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

K259E

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW186

Plasmid

#113184

Purpose

E. coli expression vector for untagged E. coli Cas1 R163A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R163A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW151

Plasmid

#113183

Purpose

E. coli expression vector for untagged E. coli Cas1 R146A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R146A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW150

Plasmid

#113182

Purpose

E. coli expression vector for untagged E. coli Cas1 S143A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

S143A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW174

Plasmid

#113181

Purpose

E. coli expression vector for untagged E. coli Cas1 R138A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R138A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW189

Plasmid

#113180

Purpose

E. coli expression vector for untagged E. coli Cas1 Q136A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

Q136A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW185

Plasmid

#113179

Purpose

E. coli expression vector for untagged E. coli Cas1 R132A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R132A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW184

Plasmid

#113176

Purpose

E. coli expression vector for untagged E. coli Cas1 R112A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R112A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW152

Plasmid

#113175

Purpose

E. coli expression vector for untagged E. coli Cas1 K12E + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

K12E

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW158

Plasmid

#113177

Purpose

E. coli expression vector for untagged E. coli Cas1 R112E + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R112E

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCYP03

Plasmid

#212836

Purpose

Expresses the Acidovorax avenae subsp. Avenae LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP10

Plasmid

#212843

Purpose

Expresses the Acidovorax avenae subsp. Avenae LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pROP-PP-FBFP

Plasmid

#122137

Purpose

FMN-based fluorescent protein Reporter plasmid for B-Rex contain perfect operator sequence (anaerobic purpose)

Depositor

Srivatsan Raman

Article

Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

Insert

FBFP

Expression

Bacterial

Promoter

J23101

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1his p66shc S36A (corrected)

Plasmid

#32575

Depositor

Toren Finkel

Article

Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

Insert

p66shc (SHC1 Human)

Tags

His

Expression

Mammalian

Mutation

S36A

Promoter

CMV

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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tre-stblHIF: pLenti-TRE-stblHIF-PGK-rtTAA-2A-Puro

Plasmid

#177203

Purpose

encoding stblHIF under inducible promoter

Depositor

Markus Covert

Article

Kudo et al bioRxiv (2022)

Insert

stblHIF

Use

Lentiviral

Expression

Mammalian

Mutation

P402A/P577G/N813A

Promoter

TRE

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB3236

Plasmid

#124708

Purpose

Expresses pTEF2-mKate2-tIDP1 and pTRX2_5xUAS-yEGFP-tCYC1 in yeast from plasdmid pRS416

Depositor

Michael Jensen

Article

Zhang et al ACS Synth Biol. 2016 Dec 16;5(12):1546-1

Insert

yEGFP

Promoter

pTRX2_5xUAS

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLRD11

Plasmid

#208263

Purpose

Production of sclareol in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
abCAS
ssSS

Expression

Bacterial

Mutation

abCAS(D537A)

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pROP-IP-FBFP

Plasmid

#122138

Purpose

FMN-based fluorescent protein Reporter plasmid for B-Rex contain imperfect operator sequence (anaerobic purpose)

Depositor

Srivatsan Raman

Article

Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

Insert

FBFP

Expression

Bacterial

Promoter

J23101

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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stblHIF: pLenti-PGK-stblHIF-PGK-Puro

Plasmid

#177202

Purpose

encoding stblHIF under PGK promoter

Depositor

Markus Covert

Article

Kudo et al bioRxiv (2022)

Insert

stblHIF

Use

Lentiviral

Expression

Mammalian

Mutation

P402A/P577G/N813A

Promoter

PGK

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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