We narrowed to 905 results for: pgem

1. pdCas9 (GB1079)

   Plasmid

   #75399

   Purpose

   Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVD1 Multiplexing Edit En-1 (GB2242)

   Plasmid

   #160564

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for the position [5_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

   Insert

   Multiplexing Edit (En-1)
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVD1 tRNA-gRNA E4-En-1 (GB2243)

   Plasmid

   #160565

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for the position [4_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

   Insert

   tRNA-gRNA position E4-En-1 (Multiplexing Edit)
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVD1 Multiplexing Dicot Edit E1_NTERM (GB2245)

   Plasmid

   #160567

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for position [D1_n] of a monocistronic tRNA-gRNA

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

   Insert

   Multiplexing Dicot Edit E1_NTERM (Multiplexing Dicot Edit)
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVD1 tRNA-gRNA position E3 (GB2240)

   Plasmid

   #160562

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for the third position (positon [D3_4]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

   Insert

   tRNA-gRNA position E3 (Multiplexing Edit)
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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