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p35S::IRE1a

Plasmid

#135232

Purpose

constitutive expression of IRE1a from A. thaliana (AT2G17520; upregulates UPR signaling)

Depositor

Armin Djamei

Article

Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

Insert

IRE1a (IRE1A Mustard Weed)

Expression

Plant

Promoter

35S

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LacI/109-pHG165c

Plasmid

#90058

Purpose

WT LacI polymorphism with T at position 109

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LacI/109

Expression

Bacterial

Mutation

109

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pY71-PT7-RiboJ-sfGFP-MGapt

Plasmid

#129119

Purpose

Expresses a fluorescent reporter for the quantification of cell-free protein expression.

Depositor

Matthew Lux

Article

Cole et al ACS Synth Biol. 2019 Sep 20;8(9):2080-20

Insert

Superfolder GFP with Malachite Green aptamer

Use

Synthetic Biology

Tags

His6 and RiboJ Insulator

Expression

Bacterial

Promoter

T7

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-DSep1

Plasmid

#174493

Purpose

bacterial expression of Drosophila Sep1

Depositor

Manos Mavrakis

Article

Mavrakis et al Nat Cell Biol. 2014 Apr;16(4):322-34. do

Insert

Sep1 (Sep1 Fly)

Tags

His6-TEV

Expression

Bacterial

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS2131

Plasmid

#111895

Purpose

ssAAV genome with MCS for inserting MiniP promoter to drive an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Type

Empty backbone

Use

AAV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pPKm- 226

Plasmid

#90497

Purpose

pcDNA3 - CMVmin - PIF3 - VPR expresses PIF3 and VPR transactivator domain , under CMV promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

PIF3-VP64

Expression

Mammalian

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pPKm-293

Plasmid

#105817

Purpose

pcDNA - TET DBD - MTAD. Expresses TET DNA binding domain fused to MTAD

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

TET DBD and MTAD

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pOTTC813 - pAAV-SYN1-CRTsigpep-GCaMP3-KDEL

Plasmid

#63886

Purpose

An AAV packaging vector that expresses ER-retained GCaMP3 under control of the SYN1 promoter.

Depositor

Brandon Harvey

Article

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

ER-localized GCaMP3(wt)

Use

AAV

Promoter

human SYN1

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_PSTPIP1-1/1

Plasmid

#91453

Purpose

Protein expression and purification of human SH3 domain construct PSTPIP1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PSTPIP1-1/1 (PSTPIP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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mKO-Fibrillarin-7

Plasmid

#57842

Purpose

Localization: Nuclear - Nucleolus, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Fibrillarin (FBL Human)

Tags

mKO

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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